Journal List > Korean J Pediatr Gastroenterol Nutr > v.6(1) > 1110410

Korean J Pediatr Gastroenterol Nutr. 2003 Mar;6(1):1-9. Korean.
Published online Mar 31, 2003.
Copyright © 2003 The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition
The Genotypes of Helicobacter pylori, Gastric Epithelial Cell Proliferation and Apoptosis in Children
Ji Ah Jung, Mi Ae Lee and Jeong Wan Seo
Department of Pediatrics, Hallym University College of Medicine, Korea.
*Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, Korea.
Department of Pediatrics, Ewha Womans University College of Medicine, Seoul, Korea.


To investigate the relation of the gastric epithelial cell proliferation, apoptosis and genotypes of H. pylori in children.


Histologic grading by updated Sydney system, PCNA immunostaining, TUNEL method and the genotypes (cagA, picB and iceA) by PCR were performed in H. pylori positive (N=20) and negative (N=20) gastric biopsy specimens.


PCNA index was significantly different between H. pylori positive children (77.4±13.12) and H. pylori negative children (52.3±12.20) (p=0.000). There were positive correlations between PCNA index and H. pylori density (r=0.624, p=0.000), polymorphonuclear neutrophil activity (r=0.460, p=0.005) and chronic inflammation (r=0.433, p=0.009). Apoptosis index of H. pylori positive children (0.70±0.411) was significantly higher than of H. pylori negative children (0.14±0.201) (p=0.000). Positive correlations between apoptosis index and H. pylori density (r=0.691, p=0.000), polymorphonuclear neutrophil activity (r=0.585, p=0.000) and chronic inflammation (r=0.535, p=0.001) were noted. As PCNA index increased, apoptosis index significantly increased (r=0.527, p=0.001). The positive rates of genotypes were cagA 90%, picB 75%, iceA1 60% and iceA2 15%, respectively. There were no significant correlations between the status of the genotypes and PCNA index, apoptosis index, the endoscopic findings and the histologic findings.


PCNA index and apoptosis index in H. pylori positive children were higher than in H. pylori negative children but were not related to H. pylori genotypes. This study suggested that correlatively increased gastric epithelial cell proliferation and apoptosis are important to pathogenesis of H. pylori infection in children.

Keywords: Helicobacter pylori; Gastric epithelial cell; Proliferation; Apoptosis; cagA; picB; iceA