Journal List > Korean J Pediatr Gastroenterol Nutr > v.9(1) > 1110228

Korean J Pediatr Gastroenterol Nutr. 2006 Mar;9(1):1-13. Korean.
Published online Mar 31, 2006.  https://doi.org/10.5223/kjpgn.2006.9.1.1
Copyright © 2006 The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition
Diagnosis of Enteropathogens in Children with Acute Gastroenteritis: One Year Prospective Study in a Single Hospital
Ju Young Chang, Ji Eun Choi, Sue Shin,* and Jong Hyun Yoon*
Department of Pediatrics, Seoul National University College of Medicine, Boramae Hospital, Seoul, Korea.
*Department of Laboratory Medicine, Seoul National University College of Medicine, Boramae Hospital, Seoul, Korea.
Abstract

PURPOSE

Acute gastroenteritis in children is one of the frequently encountered diseases with relatively high admission rate. The aim of this study is to determine the isolation trends of common and emerging pathogens in acute gastroenteritis in children over a 12-month period in a community hospital.

METHODS

The study group included the children who were hospitalized to Seoul National University Boramae Hospital from April, 2003 to March, 2004 or visited outpatient clinic from April, 2003 to July, 2003 with presenting features of acute gastroenteritis. Stool specimens were obtained within 2 days after the visit and examined for the following pathogens: rotavirus, adenovirus, Salmonella, Shigella, Vibrio, pathogenic Escherichia coli (E.coli), Campylobacter and Yersinia species. Viral study was done with commercial kits for antigen detection. Identification of the bacterial pathogens was done by culture using selective media. For pathogenic E.coli, polymerase chain reaction (PCR) was done with the target genes related to the pathogenecity of enterotoxigenic E.coli (ETEC), enteropathogenic E.coli (EPEC) and enterohemorrhagic E.coli (EHEC).

RESULTS

The 130 hospitalized children and 28 outpatients were included in this study. The majority of children (>93%) were less than 6 years. Pathogens were isolated in 47% of inpatients and 43% of outpatients, respectively. Rotavirus was the most frequently identified pathogen, accounting for 42.3% of inpatients and 29.6% of outpatients. Nontyphoidal salmonella is the most commonly isolated bacterial pathogen (3.9%) in hospitalized children. Pathogenic E.coli (EPEC, ETEC) wasdetected in 2.1% (2/97) of inpatients and 25% (3/12) of outpatients. EHEC, adenovirus, Campylobacter, Yersinia and Shigella species were not detected in this study.

CONCLUSION

Rotavirus is the most common enteropathogen in children with acute gastroenteritis. Nontyphoidal salmonella and pathogenic E.coli are important bacterial pathogens. Campylobacter species may not be commonly detected organism in hospitalized children with acute diarrhea.

Keywords: Acute gastroenteritis; Children; Enteropathogen