Journal List > Korean J Pediatr Gastroenterol Nutr > v.10(2) > 1110213

Korean J Pediatr Gastroenterol Nutr. 2007 Sep;10(2):166-172. Korean.
Published online Sep 30, 2007.
Copyright © 2007 The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition
Detection of Hepatitis B Virus DNA in Liver Grafts Obtained from HBsAb and HBcAb Positive Organ Donors
Chang Woo Jung, Joo Young Jang, Kyung Mo Kim, and Sung Gyu Lee*
Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
*Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.


It has recently been reported that de novo HBV infection following liver transplantation is caused by grafts from HBcAb positive donors, and this phenomenon has been observed in one third of the liver transplant patients in our center. Therefore, we investigated the presence of HBV virus DNA in liver tissues obtained from HBcAb positive donors to determine the mechanism by which de novo HBV infection occurs.


This study was conducted on 6 patients that were HBsAg negative, HBsAb positive, and HBcAb positive who were donors for liver transplantation between November 1997 and November 1998 at Asan Medical Center. We isolated DNA from a portion of liver biopsy tissues that were obtained during the operation, and then identified the surface and core region of HBV DNA using nested PCR. In addition, four children who received liver grafts from these donors were monitored to determine if they became afflicted with non-HBV related diseases while receiving prophylaxis consisting of short-term HBIG treatment and long-term treatment with an antiviral agent.


The surface antigen region was identified in all 6 donors and the core antigen region was observed in 4 of the 6 donors. However, no episodes of de novo HBV infection with prophylaxis were observed


The results of this study support the results of previous studies, which indicated that HBV infection may be the main cause of de novo HBV infection in patients that receive HBsAb positive and HBcAb positive donor grafts.

Keywords: Liver transplantation; Hepatitis B virus; Living donors; Hepatitis B antibodies