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Ngo, Cao, Woo, Min, and Weon: Cytotoxic Triterpenoids from the Fruits of Ligustrum japonicum
Original Article

Natural Product Sciences 2018;24(2):93-98.

Published online: 20 January 2018

DOI: https://doi.org/10.20307/nps.2018.24.2.93

Cytotoxic Triterpenoids from the Fruits of Ligustrum japonicum

Quynh-Mai Thi Ngo†, Thao Quyen Cao†, Mi Hee Woo, Byung Sun Min∗, Kwon-Yeon Weon∗

College of Pharmacy, Drug Research and Development Center, Daegu Catholic University, Gyeongbuk 38430, Republic of Korea.

∗Author for correspondence Byung Sun Min, College of Pharmacy, Drug Research and Development Center, Daegu Catholic University, Gyeongbuk 38430, Republic of Korea. Tel: +82-53-850-3613; E-mail: bsmin@cu.ac.kr Kwon-Yeon Weon, College of Pharmacy, Drug Research and Development Center, Daegu Catholic University, Gyeongbuk 38430, Republic of Korea. Tel: +82-53-850-3616; E-mail: weonky@cu.ac.kr

a† These authors contributed equally to this work

Received 16 January 2018       Revised 28 January 2018       Accepted 29 January 2018

Copyright © 2018 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Medicinal plants are potential sources of anticancer agents screening. A large number of phytochemicals, including triterpenoids, have been reported to have significant cytotoxic effects on cancer cells. From the fruits of Ligustrum japonicum Thunb., thirteen triterpenoids (1 – 13) were isolated and evaluated for their cytotoxic activity against Hela and HL-60 cells. As results, 8 (oleanolic acid) showed significant effects on Hela with IC50 values of 5.5 µM, and moderate effects on HL-60 cells with IC50 values of 55.9 µM. Meanwhile, 10 (oleanderic acid) and 11 (3β-acetoxy-urs-12-en-28-oic acid) exhibited moderate inhibitory effects on Hela with IC50 value of 55.0 and 68.8 µM, respectively. Moreover, 10 showed cytotoxic effect on HL-60 cell line with IC50 value of 63.9 µM. To our knowledge, this is the first report that oleanderic acid was isolated from L. japonicum and investigated in cytotoxic effects on Hela and HL-60 cells.

Keywords: Ligustrum japonicum, Oleaceae, Triterpenoids, Cytotoxic activity

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Fig. 1.
Chemical structures of triterpenoids isolated from L. japonicum.
nps-24-93f1.tif
Table 1.
Cytotoxic activities of compounds 1 – 13 against Hela and HL-60 cell lines.
Compds IC50, µMa Compds IC50, µMa
Hela HL-60 Hela HL-60
1 > 100 > 100 8 5.5 ± 0.7 55.9 ± 1.2
2 > 100 > 100 9 > 100 > 100
3 > 100 > 100 10 55.0 ± 2.5 63.9 ± 2.8
4 > 100 > 100 11 68.8 ± 5.1 > 100
5 > 100 > 100 12 > 100 > 100
6 > 100 > 100 13 > 100 > 100
7 > 100 > 100 Adriamycinb 0.67 ± 0.5 0.24 ± 0.06

a The results are presented as mean ± SEM (n = 3).

b Positive control.

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