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Rodriguez, Quilantang, Lee, Kim, Shim, Lee, and Lee: Determination of Silybin B in the Different Parts of Silybum marianum using HPLC-UV
Original Article

Natural Product Sciences 2018;24(2):82-87.

Published online: 20 January 2018

DOI: https://doi.org/10.20307/nps.2018.24.2.82

Determination of Silybin B in the Different Parts of Silybum marianum using HPLC-UV

Joyce P. Rodriguez1, Norman G. Quilantang1, Ju Sung Lee1, Hyun Young Kim3, Jae Suk Shim4, Sanghyun Lee1, ∗, Jeong Min Lee2

1Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea

2Natural Products Research Team, National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea

3Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, Korea

4Imsil Herbal Medicine Association, Imsil 55955, Korea

∗Author for correspondence Sanghyun Lee, Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea. Tel: +82-31-670-4688; E-mail: slee@cau.ac.kr

Received 8 September 2017       Revised 10 November 2017       Accepted 22 December 2017

Copyright © 2018 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Silymarin is the standardized extract from Silybum marianum which consists mainly of flavonoids and polyphenols. It is highly regarded for its hepatoprotective ability. Silybin B is a flavonolignan and one of the active components of silymarin. The content of silybin B in various parts of S. marianum was analyzed by HPLC-UV. Results show that the extract of seeds contain the highest amount of silybin B (7.434 mg/g DW). The petioles of S. marianum showed a low content of silybin B. This study revealed that seeds of S. marianum contain high amount of silybin B and could be a good source of the compound.

Keywords: HPLC-UV, Silybum marianum, silybin B, silymarin

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Fig. 1.
Structure of silybin B.
nps-24-82f1.tif
Fig. 2.
HPLC chromatograms of silybin B (A) and the EtOH extracts of leaves (B), petioles (C), flowers (D), stems (E), seeds (F), and germinated seeds (G) of S. marianum.
nps-24-82f2.tif
nps-24-82f3.tif
Fig. 3.
HPLC chromatograms of silybin B (A) and the EtOH extracts of seeds (B) of S. marianum
nps-24-82f4.tif
Table 1.
Calibration curve of silybin B
Compound tR Calibration equation a r2 b LOD (mg/mL) LOQ (mg/mL)
Silybin B 16.73 Y = 2,000,000X + 163,298 0.9993 1.16 3.51

a Y = peak area, X = concentration of standards (mg/mL).

b r2 = correlation coefficient for five data points in the calibration (n = 5)

Table 2.
Silybin B content of S. marianum
Sample Content (mg/g DW)
Leaves 0.113 ± 0.004
Petioles 0.043 ± 0.002
Flowers 0.083 ± 0.001
Stems 0.064 ± 0.001
Seeds 7.434 ± 0.041
Germinated Seeds 0.852 ± 0.002
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