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Abstract
Dehydroepiandrosterone(DHEA) was administered to rats to investigate the efficacy of DHEA in prevention of the process of osteoporosis induced by oophorectomy.
Female Sprague-Dawley rats, aged five months, were divided into three groups; Group 1(40 rats), sham operation as control; group 2(40 rats), bilateral oophorectomy; and group 3(20 rats), intraperitoneal injection of 10mg of DHEA every other day from 4 weeks after bilateral oophorectomy. In group 1 and 2, five rats of each group were sacrificed weekly until the 8th week. In group 3, five rats were killed weekly from 1 week to 4 weeks after DHEA administration.
Trabecular bone area(TBA) of proximal metaphysis of left tibia was obtained by quantitative image analysis system. In group 1, TBA was not changed with time after sham operation. In group 2, TBA decreased progressively through eight weeks after oophorectomy. In group 3, TBA’s were higher than those of group 2, but they were still lower than those of group 1.
These data suggested that DHEA, administered to rats from 4 weeks to 8 weeks after bilateral oophorectomy, may partially recover the osteoporosis or delay the progression of the osteoporotic change until 8 weeks after the oophorectomy.
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Fig. 1
Method of the measurement of the trabecular bone area of proximal tibia. At metaphysis of the tibia, rectangular shape of bone was selected, whose reference area was 0.196304 × 107 µm2 and breadth was approximately 2/3 of that of epiphyseal plate. Area of each trabecular bone was automatically calculated and aver aged with computer system. The total trabecular bone area of each sample was calculated by average area of each trabecular bone and number of trabecula in the rectangle.
Fig. 2A-H
Histological changes of the proximal tibia before and after oophorectomy. (Hemato-xylin-Eosin Staining, x 40 except(A) (A) 1 week after sham operation(x 20). Trabecular pattern of the osteoid is condense and well preserved in both the epiphysis and metaphysis (B) 1 week after oophorectomy. Trabecular margin become coarse, but the dense trabecular architecture is still preserved. (C) 5 weeks after oophorectomy. Trabecular bones are reduced in size and number. (D) 5 weeks after oophorectomy and 1 week after administration of DHEA. Trabecular bones are slightly larger and more abundant comparing to those of 5 weeks after oophorectomy(C). (E) 6 weeks after oophorectomy. Trabecular portion is reduced and the cellular and vacuolar portion of marrow become more predominat. (F) 6 weeks after oophorectomy and 2 weeks after administration of DHEA. Trabeculae are more abundant comparing to those of 6 weeks after oophorectomy(E). (G) 8 weeks after oophorectomy. Severe reduction in the size and the number of the trabeculae is observed. (H) 8 weeks after oophorectomy and 4 weeks after administration of DHEA. Abundant trabeculae are visible, but still less abundant than those seen in the sham operation group(A).
Fig. 3
Weekly measurement of trabecular bone area(TBA) of proximal tibiae of each group. In group 1, the TBA was not changed with time after sham operation. After oophorectomy in group 2, the TBA decreased progres sively. In group 3, TBA’s were higher than those of group 2. but still lower than those seen in group 1.1
Table 1
Weekly measurement of trabecular bone area(TBA) of proximal tibiae of each group (unit: × 105 µm2)
| 1w* | 2W | 3W | 4W | 5W | 6W | 7W | 8W | |
|---|---|---|---|---|---|---|---|---|
| group 1 | 4.23 | 3.85 | 3.92 | 4.25 | 4.01 | 3.87 | 3.97 | 4.02 |
| group 2 | 3.94 | 3.06 | 3.15 | 2.98 | 2.67 | 1.96# | 1.79# | 1.78# |
| group 3 | - | - | - | - | 2.82 | 3.03## | 2.63## | 2.77## |
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