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Han, Kim, Kim, Lee, Ohm, and Kim: Mutations of the p53 tumor suppressor gene in rheumatoid arthritis synovium
Original Article

Journal of the Korean Orthopaedic Association 2001; 36(1): 9-16.

Published online: 28 February 2001

DOI: https://doi.org/10.4055/jkoa.2001.36.1.9

Mutations of the p53 tumor suppressor gene in rheumatoid arthritis synovium

Chang Whan Han, Hyung Kwan Kim, Weon You Kim, Kwang Won Lee, Eui Yong Ohm, Jung Man Kim

Department of Orthopaedic Surgery, Taejon St. Mary's Hospital, University of Catholic, Korea.

Department of Orthopaedic Surgery, Our lady of Mercy Hospital, University of Catholic, Korea.

Department of Orthopaedic Surgery, Kang Nam St. Mary's Hospital, University of Catholic, Korea.

Copyright © by The Korean Orthopaedic Association

Abstract

PURPOSE

To analyse the expression of the p53 tumour suppressor gene in the synovial tissue from rheumatoid patients.

Material and Methods

Synovial membranes were obtained from 13 patients diagnosed as having RA, and 9 osteoarthritis (OA) patients. We studied p53 expression by immunohistochemical analysis and p53 DNA sequence using direct DNA preparation method.

RESULT

In immunohistological studies, the Do-1 monoclonal antibody stained at 6 specimens out of the 13 rheumatoid arthritis tissue biopsies analysed. There was no p53 mutation in osteoarthritis samples, but there were 4 p53 mutations from the 13 rheumatoid arthritis samples. p53 mutations were found at the codon 177 (CTG to CTA, GA), 277 (TGT to TGC, TC), and, two patients at the codon 237 (CAT to TAT, CT).

CONCLUSION

The predicted amino acid substitutions in p53 were similar to those commonly observed in a variety of tumors and might influence growth and survival of rheumatoid synoviocytes.

Keywords: Rheumatoid Arthritis, p53, Direct sequence, Immunohistochemistry

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