Journal List > Nat Prod Sci > v.24(3) > 1102537

TOOLS
Abdul, Seong, Ahn, Islam, Jung, and Choi: Anti-inflammatory Potential of Artemisia capillaris and Its Constituents in LPS-induced RAW264.7 Cells
Original Article

Natural Product Sciences 2018;24(3):171-180.

Published online: 17 January 2018

DOI: https://doi.org/10.20307/nps.2018.24.3.171

Anti-inflammatory Potential of Artemisia capillaris and Its Constituents in LPS-induced RAW264.7 Cells

Qudeer Ahmed Abdul1, Su Hui Seong1, Bo Ra Ahn1, Md Nurul Islam2, Hyun Ah Jung3, Jae Sue Choi1

1 Department of Food and Life Science, Pukyong National University, Busan 48513, Republic of Korea

2Department of Pharmacy, Mawlana Bhashani Science and Technology University, Santosh, Tangail 1902, Bangladesh

3Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju 54896, Republic of Korea

∗Author for correspondence Jae Sue Choi, Department of Food and Life Science, Pukyong National University, Busan 48513, the Republic of Korea. Tel: +82-51-629-5845; E-mail: choijs@pknu.ac.kr Hyun Ah Jung, Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju 54896, Republic of Korea. Tel: +82-63-270-4882; E-mail: jungha@jbnu.ac.kr

Received 9 March 2018       Revised 12 April 2018       Accepted 13 April 2018

Copyright © 2018 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Artemisia capillaris has been widely used as an alternative therapy for treating obesity and atopic dermatitis. It has been used as a hepatoprotactant. It is also used for ameliorating inflammatory reactions. Although there are several investigations on other Artemisia species, there is no systematic study describing the role of A. capillaris MeOH extract, its solvent soluble fractions, or derived anti-inflammatory principal components in regulating inflammatory conditions. Therefore, the objective of this study was to elucidate anti-inflammatory mechanisms of A. capillaris. Results revealed that MeOH extract of A. capillaris could decrease LPS-stimulated NO secretion. Of tested fractions, CH2Cl2, EtOAc, and n-BuOH strongly inhibited NO release from RAW264.7 cells. Bioactive mediators derived from CH2 Cl2 and n-BuOH fractions elicited potent anti-inflammatory actions and strikingly abrogated LPS-triggered NO accumulation in RAW264.7 cells. Of particular interest, capillin and isoscopoletin possessed the most potent NO suppressive effects. Western blot analysis validated the molecular mechanism of NO inhibition and showed that capillin and isoscopoletin significantly down-regulated iNOS and COX-2 protein expression. Taken together, our results provide the first evidence that MeOH extract, CH2Cl2, EtOAc, and n-BuOH fractions from A. capillaris and its derived lead candidates can potently suppress inflammatory responses in macrophages by hampering NO release and down-regulating iNOS and COX-2 signaling.

Keywords: Artemisia capillaris, Inflammation, iNOS, COX-2, Coumarin, Flavonoids

References

(1). Murakami M., Hirano T.Front. Immunol. 2012; 3:1–2.
(2). Sharma J. N., Al-Omran A., Parvathy S. S.Inflammopharmacology. 2007; 15:252–259.
(3). Bognar E., Sarszegi Z., Szabo A., Debreceni B., Kalman N., Tucsek Z., Sumegi B., Gallyas F.Jr. PLoS One. 2013; 8:e65355.
(4). Rhule A., Navarro S., Smith J. R., Shepherd D. M. J.Ethnopharmacol. 2006; 106:121–128.
(5). Tang W., Eisenbrand G.Chinese drugs of plant origin, chemistry, phamacology and use in traditional and modern medicine: Springer Verlag, New York. 1992; 179.
(6). Hong J. H., Lee J. W., Park J. H., Lee I. S.Biofactors. 2007; 31:43–53.
(7). Seo K. S., Yun K. W.Korean J. Plant Res. 2008; 21:292–298.
(8). Lee C. J., Kim H. Y., Kim J. D., Kim C. H. J.Korean Orient. Med. 2000; 21:100–107.
(9). Choi J. H., Kim D. W., Yun N., Choi J. S., Islam M. N., Kim Y. S., Lee S. M. J.Nat. Prod. 2011; 74:1055–1060.
(10). Seo K. S., Jeong H. J., Yun K. W. J.Ecol. Field Biol. 2010; 33:141–147.
(11). Cha J. D., Moon S. E., Kim H. Y., Cha I. H., Lee K. Y. J.Food Sci. 2009; 74:75–81.
(12). Hong J. H., Lee I. S.Biofactors. 2009; 35:380–388.
crossref
(13). Okuno I., Uchida K., Kadowaki M., Akahori A. Jpn. J.Pharmacol. 1981; 31:835–838.
(14). Jung H. A., Park J. J., Islam M. N., Jin S. E., Min B. S., Lee J. H., Sohn H. S., Choi J. S.Arch. Pharm. Res. 2012; 35:1021–1035.
(15). Kwon O. S., Choi J. S., Islam M. N., Kim Y. S., Kim H. P.Arch. Pharm. Res. 2011; 34:1561–1569.
(16). Nurul Islam M., Jung H. A., Sohn H. S., Kim H. M., Choi J. S.Arch. Pharm. Res. 2013; 36:542–552.
(17). Pautz A., Art J., Hahn S., Nowag S., Voss C., Kleinert H.Nitric Oxide. 2010; 23:75–93.
(18). Ribiere C., Jaubert A. M., Gaudiot N., Sabourault D., Marcus M. L., Boucher J. L., Denis-Henriot D., Giudicelli Y.Biochem. Biophys. Res. Commun. 1996; 222:706–712.
(19). Mitrovic B., Ignarro L. J., Montestruque S., Smoll A., Merrill J. E.Neuroscience. 1994; 61:575–585.
(20). Kawachi H., Moriya N. H., Korai T., Tanaka S. Y., Watanabe M., Matsui T., Kawada T., Yano H.Mol. Cell. Biochem. 2007; 300:61–67.
67.(21) Rayburn E. R.., Ezell S. J.., Zhang R.Mol. Cell Pharmacol. 2009. 1:29–43.
(22). Mulabagal V., Alexander-Lindo R. L., Dewitt D. L., Nair M. G.Evid. Based Complement. Alternat. Med. 2011; 11:1–6.
(23). Yan Y., Li J., Ouyang W., Ma Q., Hu Y., Zhang D., Ding J., Qu Q., Subbaramaiah K., Huang C. J.Cell Sci. 2006; 119:2985–2994.
(24). Turini M. E., DuBois R. N.Annu. Rev. Med. 2002; 53:35–57.
crossref
(25). Burk D. R., Cichacz Z. A., Daskalova S. M. J.Med. Plant. Res. 2010; 4:225–234.
(26). Valles J., Torrell M., Garnatje T., Garcia-Jacas N., Vilatersana R., Susanna A.Plant Biol. 2003; 5:274–284.
(27). Lim H. K., Cho S. K., Park S., Cho M. J.Korean Soc. Appl. Biol. Chem. 2010; 53:275–282.
(28). Jang M., Jeong S. W., Kim B. K., Kim J. C.BioMed. Res. Int. 2015; 2015:872718.
(29). Ali M. Y., Jannat S., Jung H. A., Choi R. J., Roy A., Choi J. S. Asian Pac. J.Trop. Med. 2016; 9:103–111.
(30). Han J., Zhao Y. L., Shan L. M., Huang F. J., Xiao X. H. Chin. J.Integr. Med. 2005; 11:54–56.
(31). Kim E. K., Kwon K. B., Han M. J., Song M. Y., Lee J. H., Lv N., Choi K. B., Ryu D. G., Kim K. S., Park J. W., Park B. H.Int. J. Mol. Med. 2007; 19:535–540.
(32). Kim Y. S., Bahn K. N., Hah C. K., Gang H. I., Ha Y. L.J. Food Sci. 2008; 73:16–20.
(33). Janbaz K. H., Gilani A. H.J. Ethnopharmacol. 1995; 47:43–47.
(34). Benli M., Kaya I., Yigit N.Cell Biochem. Funct. 2007; 25:681–686.
(35). Shahriyary L., Yazdanparast R.J. Ethnopharmacol. 2007; 114:194–198.
(36). Aniya Y., Shimabukuro M., Shimoji M., Kohatsu M., Gyamfi M. A., Miyagi C., Kunii D., Takayama F., Egashira T.Biol. Pharm. Bull. 2000; 23:309–312.
(37). Zhang Z., Guo S. S., Zhang W. J., Geng Z. F., Liang J. Y., Du S. S., Wang C. F., Deng Z. W.Ind. Crops Prod. 2017; 100:132–137.
(38). Islam M. N., Choi R. J., Jung H. A., Oh S. H., Choi J. S.Arch. Pharm. Res. 2016; 39:340–349.
(39). Tanaka K.Seikagaku. 1961; 33:399–409.
(40). Masuda Y., Asada K., Satoh R., Takada K., Kitajima J.Phytomedicine. 2015; 22:545–552.
(41). Jihed B., Bhouri W., Ben Sghaier M., Bouhlel I., Kriffi M., Skandrani I., Dijoux F. M., Ghedira K., Chekir-Ghedira L.Nutr. Cancer. 2012; 64:1095–1102.
(42). Kiso Y., Ogasawara S., Hirota K., Watanabe N., Oshima Y., Konno C., Hikino H.Planta Med. 1984; 50:81–85.

Fig. 1.
Structures of compounds isolated from Artemisia capillaris.
nps-24-171f1.tif
Fig. 2.
Effects of MeOH extract on cell viability and LPS-induced nitrite formation in RAW264.7 cells. Cell viability was measured by MTT assay.
nps-24-171f2.tif
Fig. 3.
Effects of solvent soluble fractions of MeOH extract from Artemisia capillaris on cell viability (a) and LPS-induced nitrite formation (b) in RAW264.7 cells. Cells were pre-treated with different concentrations (10, 50 and 100 μg/mL) of CH2Cl2 (C), EtOAc (E), BuOH (B), or H2 O (H) fraction of MeOH extract from Artemisia capillaris followed by treatment with LPS (1 μg/ mL) and incubation for 18 h.
nps-24-171f3.tif
Fig. 4.
Effects of capillin (a), scopoletin (b), isoscopoletin (c), scopolin (d), isoscopolin (e), or scoparone (f) isolated from Artemisia capillaris on cell viability and LPS-induced NO production in RAW264.7 cells.
nps-24-171f4.tif
Fig. 5.
Effects of arcapillin (a), quercetin 3-O-robinobioside (Q3R) (b), isorhmanetin 3-O-robinobioside (IR3R) (c), or quercetin 3-O-galactopyranosyl 7-O-α-L-rhamnoside (QGR) (d) isolated from Artemisia capillaris on cell viability and LPS-induced NO production in RAW264.7 cells.
nps-24-171f5.tif
Fig. 6.
Effects of capillin (a) or isoscopoletin (b) on the production of iNOS and COX-2 protein in RAW264.7 cells.
nps-24-171f6.tif
TOOLS
Similar articles