Carbon monoxide releasing molecule-2 protects mice against acute kidney injury through inhibition of ER stress

Md Jamal Uddin; Eun Seon Pak; Hunjoo Ha

doi:10.4196/kjpp.2018.22.5.567

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Uddin, Pak, and Ha: Carbon monoxide releasing molecule-2 protects mice against acute kidney injury through inhibition of ER stress

Original Article

The Korean Journal of Physiology & Pharmacology 2018; 22(5): 567-575.

Published online: 27 August 2018

DOI: https://doi.org/10.4196/kjpp.2018.22.5.567

Carbon monoxide releasing molecule-2 protects mice against acute kidney injury through inhibition of ER stress

Md Jamal Uddin, Eun Seon Pak, Hunjoo Ha

Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Korea.

Correspondence: Hunjoo Ha. hha@ewha.ac.kr

Received 5 April 2018 Revised 28 June 2018 Accepted 12 July 2018

The Korean Physiological Society and The Korean Society of Pharmacology

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Acute kidney injury (AKI), which is defined as a rapid decline of renal function, becomes common and recently recognized to be closely intertwined with chronic kidney diseases. Current treatment for AKI is largely supportive, and endoplasmic reticulum (ER) stress has emerged as a novel mediator of AKI. Since carbon monoxide attenuates ER stress, the objective of the present study aimed to determine the protective effect of carbon monoxide releasing molecule-2 (CORM2) on AKI associated with ER stress. Kidney injury was induced after LPS (15 mg/kg) treatment at 12 to 24 h in C57BL/6J mice. Pretreatment of CORM2 (30 mg/kg) effectively prevented LPS-induced oxidative stress and inflammation during AKI in mice. CORM2 treatment also effectively inhibited LPS-induced ER stress in AKI mice. In order to confirm effect of CO on the pathophysiological role of tubular epithelial cells in AKI, we used mProx24 cells. Pretreatment of CORM2 attenuated LPS-induced ER stress, oxidative stress, and inflammation in mProx24 cells. These data suggest that CO therapy may prevent ER stress-mediated AKI.

Keywords: Acute kidney injury, Carbon monoxide, ER stress, Oxidative stress

INTRODUCTION

Acute kidney injury (AKI) is responsible for about 2 million deaths each year worldwide, and its incidence is rising [1]. In contrast to the traditional belief that survivors of AKI tend to fully recover renal function, there is growing evidence that patients who survive an episode of AKI might have a significant risk of developing progressive chronic kidney diseases [2 3]. Interventions to prevent poor patient outcomes during AKI are urgently needed, beside detecting AKI at early stages [4].

Endoplasmic reticulum (ER) stress [5 6 7] plays an important role in pathogenesis of AKI, along with reactive oxygen species (ROS) [8 9], apoptosis [10] and inflammation [11]. During ER stress, the activated unfolded protein response (UPR) is associated with RNA-dependent protein kinase-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 (IRE1). The endoribonuclease activity of IRE1 cleaves XBP1 to generate spliced XBP1 (sXBP1), a potent transcription factor [12]. Active XBP1 in turn induces the expression of UPR target genes, and stimulates the production of inflammatory cytokine genes [13]. IRE1 is also responsible for activation of JNK during ER stress [14]. Additionally, ROS generation is associated with ER stress [15 16]. Therefore, targeted inhibition of ER stress may hold promise as a new strategy in the fight against AKI.

Carbon monoxide (CO) is an endogenously produced gas resulting from the degradation of heme by heme oxygenase. While high concentration of CO represents a toxic inhalation hazard, low-dose of CO (~250–500 parts per million) shows protective functions in preclinical models of human diseases [17]. For instance, inhalation of CO attenuates inflammation and apoptosis in acute lung injury, ischemia reperfusion (I/R) injury in liver/ lung/heart, vascular injury and graft rejection [18]. CO also attenuates oxidative stress-induced inflammation and fibrosis in the hypoxic lungs [19]. CO or CO releasing molecule-2/3 (CORM2/3) inhibits ER stress in endothelial cells, splenic macrophages, hepatocytes, fibroblasts and ilets [20 21]. In the kidney, CO exerts protective effects against fibrosis induced by unilateral ureteral obstruction in mice [22]. In addition, CO/CORMs can be beneficial for treating AKI induced by endotoxin or sepsis [23 24], cisplatin [25], transplantation [26] as well as ischemia-reperfusion [27 28] by suppressing oxidative injury, reducing cell apoptosis, and increasing cell survival. Although the protective effect of CO has been studied in AKI, the detailed mechanism remains elusive.

However, there is no study on effect of CO on ER stress in AKI. Since that ER stress has emerged as a novel biomarker for AKI and that CO therapy is a potential candidate for treatment of AKI, the current study aimed to determine whether CO therapy attenuates AKI through suppression of ER stress. We used lipopolysaccharide (LPS)-induced AKI among AKI mouse models such as LPS, cisplatin, cecal ligation and puncture (CLP), and ischemia reperfusion injury (IRI), since LPS model mimics many features of sepsis in human [29 30 31], decreases glomerular filtration rate (GFR), increases blood urea nitrogen (BUN), and increases neutrophil infiltration [32].

METHODS

Materials

Chemicals and antibodies were obtained from Sigma-Aldrich Company (St. Louis, MO, USA) and Cell Signaling Technology (Danvers, MA, USA), respectively, unless otherwise stated.

Animals

Six-week-old male C57BL/6 mice (Japan SLC Inc., Hamamatsu, Japan) were used in this study. In 1^st series, mice were divided into four groups: (i) control, (ii) LPS 6 h, (iii) LPS 12 h, and (iv) LPS 24 h. Induction of AKI was evaluated at 6, 12, and 24 h with single intraperitoneal (i.p.) injection of LPS (15 mg/kg) [33]. In 2^nd series, mice were divided into four groups: (i) control, (ii) CORM2, (iii) LPS, and (iv) LPS treated with CORM2. AKI was induced by single i.p. injection of 15 mg/kg LPS for 18 h. Age-matched control mice were injected with an equivalent volume of saline as vehicle of LPS. CORM2 at 30 mg/kg was administered (i.p.) to mice 2 h before administration (i.p.) of LPS, and age-matched control mice were injected with an equivalent volume of dimethyl sulfoxide (DMSO) as vehicle of CORM2 as described [33]. Mice were monitored at every 2 h interval during the experimental period. All mice were sacrificed at the above mentioned time point after LPS injection via anesthesia with 16.5% urethane (10 ml/kg). All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC No. 14-051) of Ewha Womans University.

Measurement of blood parameters

Blood samples were collected with a heparinized syringe before mice were sacrificed. Plasma creatinine level was determined using a Detect X Serum Creatinine Detection Kit (Arbor Assays, Ann Arbor, MI, USA. Cystatin C level was measured using a cystatin C Elisa kit (R&D systems, Minneapolis, MN, USA). Plasma LPO level was measured by reacting with thiobarbituric acid as described previously [34].

Measurement of kidney LPO

LPO in kidney tissue was measured with the LPO assay kit (Cayman Chemical Co, Ann Arbor, MI, USA) according to the manufacturer's recommended protocols.

Direct measurement of ROS

Frozen section of kidney tissues from AKI mice treated with or without CORM2 were used. Dihydroethidium (DHE) (5 µM, Molecular Probes, Eugene, Oregon, USA) was applied to the frozen section of kidneys (5 µm) for 10 min at 37℃ to reveal the presence of ROS with red fluorescence at 561 nm followed by DAPI staining and images were taken using a Zeiss ApoTome Axiovert 200M microscope (Carl Zeiss Microscopy GmbH, 07745 Jena, Germany).

Immunohistochemistry

For immunohistochemistry, we used anti-F4/80 (1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and anti-nitrotyrosine (1:200; Santa-Cruz Biotechnology) antibodies. Images were captured using a Zeiss microscope equipped with an Axio Cam HRC digital camera and Axio Cam software (Carl Zeiss, Thornwood, NY, USA). Staining intensities were then quantified using Image-Pro Plus 4.5 software (Media 149 Cybernetics, Silver Springs, MD, USA) as described previously [35].

Cell culture

Immortalized mProx24, mouse proximal tubular epithelial cells were cultured as described previously [36]. Subconfluent cells were pretreated with CORM2 (0, 5, 10, and 20 µM, dissolved in DMSO) for 2 h and then, stimulated with LPS (100 ng/ml, dissolved in distilled water) for 6 h.

Real-time RT-PCR analysis

Total RNA was extracted using Trizol reagent (Life Technologies, Carlsbad, CA, USA), and mRNA expression was measured by means of real-time PCR using an ABI7300 system (Applied Biosystems, Carlsbad, CA, USA) and 20 µl reaction volumes containing cDNA transcripts, primer pairs, and SYBR Green PCR Master Mix (Applied Biosystems) as described previously [35]. Primer sequences are shown in Table 1.

Western blot analysis

pNF-κB p65, NF-κB, pJNK, JNK, CHOP, and β-actin proteins were measured by standard western blot analysis as described previously [35] using anti-pNF-κB p65 (1:5000, Cell Signaling Technology), anti-NF-κB (1:4000, Cell Signaling Technology), anti-pJNK (1:5000, Cell Signaling Technology), anti-JNK (1:3000, Cell Signaling Technology), anti-CHOP (1:1000, Santa-Cruz Biotechnology), and anti-β-actin (1:1000) antibodies.

Statistical analysis

All results are expressed as mean±standard error (SE). Statistical significance of differences among groups were compared by analysis of variance followed by Fisher post-hoc analysis. Statistical significance was considered when p value was less than 0.05.

RESULTS

CORM2 attenuates LPS-induced kidney injury

First, our study showed that time dependent treatment of LPS significantly decreased kidney weight to body weight (Fig. 1A) as well as increased markers of kidney injury such as plasma creatinine (Fig. 1B) and cystatin C (Fig. 1C). Though plasma cystatin C started to increase at 6 h, plasma creatinine increased at 12 h and both of them reached apparently at pick level at 24 h. Considering plasma creatinine, cystatin C or body condition, we decided 18 h time point for optimum AKI induction for further study. Pretreatment of CORM2 significantly reduced LPS-induced plasma creatinine (Fig. 1D) and cystatin C (Fig. 1E) levels, indicating that CORM2 may improve kidney function in mice. Then, we examined the tubular damage by analyzing the expression of kidney injury molecule (KIM1) levels. LPS-induced AKI mice showed increased plasma KIM1 levels (Fig. 1F) while CORM2 treatment significantly inhibited the effects (Fig. 1F).

CORM2 attenuates LPS-induced inflammation in the kidney

As shown in Fig. 2A, the master regulator of inflammation, pNF-κB markedly increased by LPS while CORM2 significantly decreased the effects in the kidneys (Figs. 2A and B). Macrophage infiltration as measured by F4/80 staining was remarkably increased by LPS and CORM2 treatment significantly decreased the effects in the kidneys (Figs. 2C and D). As expected, mRNAs of LPS-induced proinflammatory cytokines such as TNFα, MCP1, iNOS, and ICAM1 were significantly inhibited by CORM2 in the kidneys (Fig. 2E). Since pathophysiological role of tubular epithelial cells in AKI has been implicated [37 38], we used mProx cells in our study to confirm the effect of CORM2. Consistently, mRNAs of LPS-induced proinflammatory cytokines were also significantly inhibited by CORM2 in the mProx cells (Fig. 2F).

CORM2 inhibits LPS-induced ER stress in the kidney

Since ER stress has emerged as a novel biomarker of AKI, we examined the effect of CORM2 on the downstream signaling of ER stress. Interestingly, CORM2 significantly decreased LPS-induced p-JNK (Figs. 3A and B) and CHOP (Figs. 3C and D) protein expression in the kidneys. In addition, mRNAs of ER stress markers such as spliced X-box-binding protein 1 (sXBP1), ER-degradation-enhancing-α-mannidose-like protein1 (Edem1), and glucose-regulated protein 78 (GRP78) were significantly attenuated by CORM2 in the AKI kidneys (Fig. 3E). Further to confirm the effect of CORM2 on ER stress markers, we used mProx cells. Consistently, mRNAs of LPS-induced ER stress markers such as sXBP1, Edem1, and GRP78 were also significantly inhibited by CORM2 in the mProx cells (Fig. 3F).

CORM2 inhibits LPS-induced ROS generation in the kidney

To confirm the anti-oxidative effect of CORM2, we measured plasma and kidney lipid hydroperoxide (LPO). Interestingly, CORM2 significantly inhibited LPS-induced plasma and kidney LPO (Figs. 4A and B). LPS-induced nitrotyrosine accumulation was also significantly decreased by CORM2 treatment in the kidney tissue (Fig. 4C). In addition, DHE staining was performed to directly measure ROS in kidney tissues. LPS effectively induced ROS generation which was significantly inhibited by CORM2 treatment (Fig. 4D). The mRNA levels of antioxidant genes such as catalase and Prx1 were inhibited by LPS while CORM2 treatment significantly increased their expression (Figs. 4E and F). CO is known to increase antioxidants such as NRF2, HO1, and NQO1. Consistently, mRNA levels of NRF2, HO1, and NQO1 were significantly increased by CORM2 in LPS-treated kidneys in mice (Fig. 4G). Consistently, the mRNA levels of NRF2, HO1, and NQO1 were also significantly increased by different doses of CORM2 in LPS treated mProx cells (Fig. 4H).

DISCUSSION

The present data demonstrated that administration of CORM2 could exert a protective effect against LPS-induced AKI by inhibiting ER stress.

LPS induces systemic inflammation which mimics many features of sepsis in human [29 30 31]. In addition, LPS administration is simple and sterile [31] which are the important parameters for an experimental model. With respect to AKI, LPS injection causes decreased GFR, increased BUN, and increased neutrophil infiltration [32]. Thus, we employed LPS-induced AKI model in this study.

Protective effect of CORM2 on LPS-induced AKI is consistent with the previous studies [23 24 25 26 27 28]. CORM2 protects CLP [24]- and IRI [27]-induced decreased GFR and inflammation in rats and mice. In addition, CO/CORMs inhibits AKI induced by endotoxin [23], cisplatin [25], and transplantation [26] by suppressing oxidative injury, and reducing cell apoptosis. In addition, high pressure CO preserves rat kidney graft from apoptosis and inflammation [39].

CO is known to inhibit ER stress in different cell types including endothelial cells, splenic macrophages, hepatocytes, fibroblasts and ilets [20 21]. The present data provide the implication of CO on ER stress-mediated AKI. Considering i) the involvement of ER stress in apoptosis leading to renal tubular epithelial cell injury [10], and ii) ER stress-mediated apoptosis in response to LPS [40 41], we have measured CHOP and pJNK expression as the apoptosis marker in kidney tissues. LPS-induced CHOP and pJNK expression was effectively inhibited by CORM2 under our experimental conditions, suggesting the involvement of apoptosis in ER stress-induced AKI. In addition, others markers of ER stress were reduced by CORM2 in the kidneys. However, the detailed mechanisms involved in CO-mediated suppression of ER stress have not yet been established.

From mechanical point of view, CO is membrane-permeable and directly binds to metal containing proteins such as NADPH oxidase, soluble guanylate cyclase (sGC), cytochrome P450 proteins, cytochrome c oxidase, catalase, peroxidase, and nitric oxide synthase [42]. The binding of CO to these proteins is able to confer a conformational change which can alter their biological activity [43 44 45]. CO activates different signaling such as PI3K-AKT, PKC, PPAR-γ and MAPK which are involved in inhibition of inflammation and apoptosis [46 47]. CO-mediated suppression of ER stress has been implicated in endothelial cells through activation of Nrf2/HO1 and p38 MAPK [20]. Another important signaling pathway for CO is alteration of ROS generation. Cytosolic CO inhibits the activity of NADPH oxidase and thereby, reduces superoxide production [45 48]. Since ROS is associated with ER stress, CO may play an important role in inhibition of ER stress in AKI. Treatment of CORM2 not only effectively decreased oxidative stress but also significantly increased anti-oxidant genes level in LPS-treated AKI mice.

The mRNA expression of HO1, an antioxidant was increased in kidney tissues in response to LPS under our experimental conditions as shown in Fig. 4G. Decreased or deficiency of antioxidative genes such as Nrf2, HO1, and NQO1 is commonly associated with different disease pathologies including kidney injury [49 50 51 52]. On the other hand, the increased levels of Nrf2, HO1, and NQO1 are involved in adaptive responses against oxidative stress after LPS treatment in AKI mice, human monocytic cells, and cisplatin-induced AKI in rats or other kidney injury models [53 54 55 56 57]. Further studies with knockdown of the molecules (i.e., Nrf2 or HO1) using siRNA are needed to strengthen the findings.

However, several questions related to our findings remain to be answered such as a) therapeutic effect of CORM2 on AKI was not investigated in the present study, b) the products of decomposition of CORM2 can generate additional effects which may contribute to the observed biological outcome, c) the optimal delivery, dosing protocol, as well as the treatment for human diseases with CO are not yet established.

In conclusion, our findings and existing evidences support that ER stress and oxidative stress inhibition by CORM2 might have protective potential in AKI. Kidney injury, ER stress, oxidative stress, and inflammation were all elevated in LPS-treated kidneys which were significantly prevented by CORM2. These results suggest that CORM2 treatment aimed at preventing ROS-mediated ER stress may hold promise to alleviate the high morbidity and mortality associated with AKI.

Figures and Tables

Fig. 1

CORM2 attenuates LPS-induced kidney injury.

To fix the optimum time required for AKI induction, mice were treated with LPS (15 mg/kg) in a time dependent manner (0, 6, 12, 24 h). (A) Kidney weight/body weight (g), (B) Plasma creatinine (mg/dl), and (C) Plasma cystatin C (ng/ml). After fixing proper AKI induction time as 18 h, we pretreated mice with CORM2 (30 mg/kg) and then treated with LPS (15 mg/kg) for 18 h. (D) Plasma creatinine (mg/dl), (E) Plasma cystatin C (ng/ml), and (F) Tubular injury marker, plasma Kim1 (pg/ml). Data are presented as means±SE of 5–8 mice/group; ^*p<0.05 vs. control, ^†p<0.05 vs. LPS.

Fig. 2

CORM2 attenuates LPS-induced inflammation in kidney.

(A) pNF-κB protein expression in the kidney was measured using western blotting. (B) Band intensities were measured using ImageJ software. (C, D) Paraffin-embedded kidney sections were stained with anti-F4/80 antibodies (1:200; original magnification: 100×; scale bar: 100 µm), and arrow marks represents the macrophage infiltration. (E) The mRNA levels of inflammation markers such as TNFα, MCP1, iNOS, and ICAM1 in kidney tissue were measured using real-time PCR. Data are presented as means±SE of 5–8 mice/group; ^*p<0.05 vs. control, ^†p<0.05 vs. LPS. (F) The mRNA levels of inflammation markers such as TNFa, MCP1, iNOS, and ICAM1 in mProx cells were measured using real-time PCR. Data are presented as means±SE, n=4; ^*p<0.05 vs. control, ^†p<0.05 vs. LPS.

Fig. 3

CORM2 inhibits LPS-induced ER stress in kidney.

(A) pJNK protein expression was measured in the kidney using western blotting, and (B) band intensities were measured using ImageJ software. (C) CHOP protein expression was measured in the kidney using western blotting, and (D) band intensities were measured using ImageJ software. (E) ER stress markers such as sXBP1, Edem1, and GRP78 mRNA levels were measured in kidney tissue using realtime PCR. (A–E) Data are presented as means±SE of 5–8 mice/group; ^*p<0.05 vs. control, ^†p<0.05 vs. LPS. (F) The mRNA levels of ER stress markers such as sXBP1, Edem1, and GRP78 in mProx cells were measured using real-time PCR. Data are presented as means±SE, n=4; ^*p·0.05 vs. control, ^†p<0.05 vs. LPS.

Fig. 4

CORM2 inhibits LPS-induced ER stress through suppression of ROS generation.

(A) Plasma LPO (µM), and (B) Kidney LPO (µM/mg tissue). (C) Paraffin-embedded kidney sections were stained with anti-nitrotyrosine antibodies (1:200; original magnification: 200×; scale bar: 100 µm), and arrow marks represents the accumulation of nitrotyrosine (brown color) on cytosol. (D) Frozen kidney sections were stained with DHE dye (5 µM for 10 min; original magnification: 100×; scale bar: 50 µm), and red color indicates the accumulation of ROS. The mRNA levels of antioxidant genes such as (E) Catalase, (F) Prx1, and (G) NRF2, HO1, and NQO1 in kidney tissue were measured using real-time PCR. (A–G) Data are presented as means±SE of 5–8 mice/group; ^*p<0.05 vs. control, ^†p<0.05 vs. LPS. (H) The mRNA levels of antioxidant genes such as NRF2, HO1, and NQO1 in mProx cells were measured using real-time PCR. Data are presented as means±SE, n=4; ^*p<0.05 vs. control, ^†p<0.05 vs. LPS.

Table 1

Primers used for real time RT-PCR analysis

ACKNOWLEDGEMENTS

This work is supported by the grants from National Research Foundation (2017R1D1A1B03028835) and Korean Research Fellowship program (2015H1D3A1062189), Republic of Korea.

Notes

^{CONFLICTS OF INTEREST} The authors declare no conflicts of interest.

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