Anti-pseudomonal Effect of a Nephrite-containing Contact Lens Storage Case

Sang Min Lee; Jae Woo Jung; Dong Hyun Lee; Sung Hee Park; Jong Heon Lee; Hak Sun Yu; Yoon Kyung Kim; Ji-Eun Lee

doi:10.3341/jkos.2018.59.8.724

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Lee, Jung, Lee, Park, Lee, Yu, Kim, and Lee: Anti-pseudomonal Effect of a Nephrite-containing Contact Lens Storage Case

Original Article

J Korean Ophthalmol Soc 2018;59(8):724-729.

Published online: 25 September 2018

DOI: https://doi.org/10.3341/jkos.2018.59.8.724

Anti-pseudomonal Effect of a Nephrite-containing Contact Lens Storage Case

Sang Min Lee, MD¹, Jae Woo Jung, MD¹, Dong Hyun Lee, MD¹, Sung Hee Park, MS², Jong Heon Lee, MD¹, Hak Sun Yu, PhD², Yoon Kyung Kim, PhD³, Ji-Eun Lee, MD, PhD^1,^4,

¹Department of Ophthalmology, Pusan National University Yangsan Hospital, Pusan National University School of Medicine, Yangsan, Korea

²Department of Parasitology and Tropical Medicine, Pusan National University School of Medicine, Yangsan, Korea

³Department of Optometry, Busan Women's College, Busan, Korea

⁴Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Pusan National University School of Medicine, Yangsan, Korea

Address reprint requests to Ji-Eun Lee, MD, PhD Department of Ophthalmology, Pusan National University Yangsan Hospital, #20 Geumo-ro, Mulgeum-eup, Yangsan 50612, Korea Tel: 82-55-360-2590, Fax: 82-55-360-2161 E-mail: jiel75@hanmail.net

Received 14 February 2018 Revised 20 May 2018 Accepted 20 July 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To compare the anti-pseudomonal effect of nephrite-containing contact lens (CL) storage cases with conventional CL storage cases.

Methods

The degree of proliferation and adhesion of Pseudomonas aeruginosa (P. aeruginosa) on 1st–3rd generation silicone hydrogel contact lenses (SHCLs) was compared by using both 5% nephrite-containing CL storage cases and conventional CL storage cases. The degree of adhesion of P. aeruginosa to the CL was also compared in 1% or 5% nephrite solution and multipurpose solution (MPS).

Results

Nephrite-containing CL storage cases showed a significant inhibitory effect on P. aeruginosa proliferation (p = 0.03) and significantly reduced the number of adherent P. aeruginosa on all 1st–3rd generation SHCLs compared with conventional CL storage cases (p = 0.02, p = 0.03, and p < 0.01, respectively). There was no significant anti-pseudomonal effect of 1% and 5% nephrite solutions compared with MPS.

Conclusions

Nephrite may be the next generation substance that reduces infectious keratitis caused by P. aeruginosa when added to CL storage cases.

Keywords: Contact lens storage case, Infectious keratitis, Multipurpose solution, Nephrite, Pseudomonas aeruginosa

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Figure 1.

Representative photographs of bacterial colonies on Luria-Bertani agar plate. The number of Pseudomonas aeruginosa colonies was significantly reduced in 5% contact lens (CL) storage cases containing 5% nephrite (B) compared with traditional CL storage cases (A).

Figure 2.

Number of Pseudomonas aeruginosa (P. aeruginosa) on contact lens (CL) storage cases. CL storage cases containing 5% nephrite significantly reduced the numbers of P. aeruginosa compared with traditional CL storage cases. Using a unpaired t-test to compare the differences between the two groups.

FIgure 3.

Representative photographs of bacterial colonies on Luria-Bertani agar plate. These pictures (A-F) show the decreased number of Pseudomonas aeruginosa colonies in all generations of silicone hydrogel contact lenses stored at contact lens (CL) storage cases containing 5% nephrite.

Figure 4.

The number of Pseudomonas aeruginosa (P. aeruginosa) adhering to contact lenses. The number of P. aeruginosa adhering to contact lenses in contact lens storage case containing 5% nephrite was significantly reduced compared with tra-ditional contact lens storage case regardless of the generation of silicone hydrogel contact lenses. Using a unpaired t-test to compare the differences between the two groups.

Figure 5.

Relationship of the number of Pseudomonas aeruginosa colonies among contact lenses treated with multipurpose solution and 1% and 5% nephrite solutions. Nephrite solutions showed a lot of colonies compared with optifree solution, regardless of the generation of silicone hydrogel contact lenses. Using a unpaired t-test to compare the differences between the two groups.

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