Prostate-specific membrane antigen-targeting monoclonal antibodies (mAbs) were investigated in the late 90's. ¹¹¹In-capromab pendetide (ProstaScint®) was approved by the FDA for the evaluation of patients with biopsy-proven PCa with high-risk of pelvic lymph node (LN) metastasis. However, the overall diagnostic accuracy of ProstaScint® fell short, since the murine mAb used for ProstaScint® (7E11-C5.3) binds to an intracellular epitope of the PSMA (15), which may be accessible only in dead, dying, or apoptotic cells within tumors. Accordingly, the next generation anti-PSMA mAbs were designed to bind to the extracellular portion of PSMA to enhance diagnostic accuracy. Besides targeting affinity and specificity, drawbacks are still remained in the use of radiolabeled mAbs as an effective diagnostic tool. Their long biological half-life in the circulation and poor penetrating ability to the solid tumor result in high non-specific background-to-tumor noise and reduce diagnostic accuracy.

Immuno-PET has been introduced to overcome the limitations of conventional antibody imaging, via modification of antibodies and selection of appropriate positron emitters. The half-life of a positron emitter should be long enough to achieve optimal background-to-tumor ratios, and an antibody or antibody fragment should be sufficiently maintained during in vivo binding. In this regard, studies have investigated biologically-engineered single chain fragments or minibodies combined with the longer-lived positron emitters such as ⁸⁹Zr and ⁶⁴Cu for immuno-PET. The introduction of immuno-PET is also an attractive novel option for PSMA-targeting imaging. The humanized antibody J591 (huJ591) directly targets the extracellular domain of the PSMA, and the modified form of huJ591 labeled with ⁸⁹Zr showed promising results in clinical trials (22). The minibody IAB2M, genetically engineered from huJ591, labeled with ⁸⁹Zr demonstrated superior results for the detection of bone metastases, compared to conventional imaging modalities in clinical phase I trial (22).

The PSMA has a unique feature that forms a ligand-receptor complex with a substrate. Based on this feature, small molecules mimicking the endogenous substrate for the PSMA that are labeled with radionuclides have been developed for the diagnosis and treatment. The basic chemical structure of these PSMA ligands incorporates glutamate-urea-glutamate or glutamate-urea-lysine dimers, which are essential structural components required for binding to the catalytic domain of PSMA (23). The first generation of PSMA ligands was glutamate-urea amino acid heterodimeric inhibitors of the PSMA, which was initially developed for scintigraphy and/or SPECT (24). Thereafter, PSMA ligands linked with a chelator for ⁶⁸Ga complexation were developed for PET imaging, as PET imaging is much advantageous over scintigraphic imaging in terms of image resolution and quantification. In addition to ⁶⁸Ga-labeled PSMA ligands, ¹⁸F-labeled PSMA ligands are available for clinical use. These PSMA ligands radiolabeled with positron emitters are of utmost clinical interest for both the diagnosis and treatment of PCa and will be discussed as a main topic in this review.

Prostate-specific membrane antigen ligands that are labeled with ⁶⁸Ga, a positron emitter, are promising and widely available in clinical practice. Among ⁶⁸Ga-PSMA ligands, ⁶⁸Ga labeled with Glu-NH-CO-NH-Lys-(Ahx) (⁶⁸Ga-HBED-CC or ⁶⁸Ga-PSMA-11) is a leading PET tracer that is the most widely used and actively investigated in clinical settings. ⁶⁸Ga-PSMA-11 has a strong binding affinity for the PSMA and is efficiently internalized into prostate tumor cells (25). Several biodistribution studies of ⁶⁸Ga-PSMA-11 well demonstrated cellular expressions of PSMA across the body; in the lacrimal and salivary glands, liver, spleen, kidneys, and some parts of the intestines (262728) (Fig. 1). The uptake of ⁶⁸Ga-PSMA-11 in these tissues is considered physiological, and the expression level of PSMA are markedly below than that of prostate tumor cells (29). On the other hand, unbound form of ⁶⁸Ga-PSMA-11 is excreted via the kidneys and urinary tract (30). Another ⁶⁸Ga-PSMA ligands such as ⁶⁸Ga-PSMA-617, and ⁶⁸Ga-PSMA-I&T have demonstrated similar biodistribution and imaging properties to ⁶⁸Ga-PSMA-11. Due to their similarities each other, ⁶⁸Ga-PSMA-11, ⁶⁸Ga-PSMA-617, and ⁶⁸Ga-PSMA-I&T are collectively known as ⁶⁸Ga-PSMA ligands. ⁶⁸Ga-PSMA ligands are advantageous over anti-PSMA antibodies since they are “small molecules.” These ligands possess high receptor affinity for the PSMA as aforementioned; they have excellent tissue penetrating abilities, and then diffuse well into solid tumor lesions such as bone metastases of PCa.

Although ⁶⁸Ga-PSMA ligands have prevailed in the studies for the development of PCa imaging, there appears to be growing interests in developing ¹⁸F-PSMA ligands. In general, ¹⁸F-based PET would offer advantages over ⁶⁸Ga-based PET with respect to availability, amount of production, and image resolution (Fig. 1). The first-generation ¹⁸F-PSMA ligand was N-[N-[(S)-1,3-dicarboxyprophyl]carbamoyl]-4-[¹⁸F]-fluorobenzyl-L-cysteine (¹⁸F-DCFBC), and a first-in-human study of ¹⁸F-DCFBC was performed in 5 patients with metastatic PCa (31). In this study, ¹⁸F-DCFBC had favorable dosimetry and a good biodistribution profile and was able to detect putative sites of occult disease that were not defined by conventional imaging modalities. However, ¹⁸F-DCFBC had persistently high blood-pool activity and relatively low background-to-tumor ratios. To overcome these limitations, the second generation ¹⁸F-PSMA ligand, 2-(3-(1-carboxy-5-((6-[¹⁸F]fluoro-pyridine-3-carbonyl)amino)-pendtyl)-ureido)-pentanedioic acid (¹⁸F-DCFPyL) was clinically introduced (3233). A first-in-human study of ¹⁸F-DCFPyL was performed in 9 patients with metastatic PCa; this ligand also exhibited favorable dosimetry, biodistribution, and safety profiles (33). Most recently, ¹⁸F-PSMA-1007 was developed so as to improve the biodistribution profiles of the previously developed ¹⁸F-PSMA ligands. ¹⁸F-PSMA-1007 reduces urine clearance, which could potentially facilitate the evaluation of the prostatic bed (34).

Biochemical recurrence refers to the clinical status of an increasing serum PSA level after curative intent of local treatments in PCa patients. Currently, BCR is defined as two consecutive values of PSA > 0.2 ng/mL in patients receiving radical prostatectomy (RP). BCR is a commonly encountered but very difficult clinical situation for clinicians, because both the early detection of treatment failure and exact localization of tumor recurrence are important in treating patients with BCR. Different therapeutic options are available for the treatment of BCR after RP, according to the disease extent; a locoregional disease vs. systemic disease. In the absence of systemic disease, salvage radiation therapy could be the first treatment option for patients with BCR. In addition, metastasis-directed therapies could enhance therapeutic outcomes and reduce unnecessary side effects when administered at lower PSA levels (35). In particular, it has been reported that the patient's prognosis was improved when the salvage therapy was initiated before the PSA level exceeds 0.5 ng/mL (36). Therefore, there is a rising demand for a reliable and accurate diagnostic tool to detect tumor recurrence and assess its extent in patients with BCR.

Prostate-specific membrane antigen PET can detect recurrence sites at lower PSA levels than conventional imaging modalities, as well as localize the origin of PSA increase, because it is important to distinguish local recurrence and systemic metastases in order to plan therapeutic approach. Although MRI has proven to be useful in the detection of locoregional recurrence of PCa, but PSA levels in the studies were generally higher than those in PSMA PET studies. Several retrospective studies using MRI demonstrated high diagnostic accuracies with sensitivities of 53–95% in the detection of BCR, but mean PSA levels ranged from 1.23 ng/mL to 2.18 ng/mL (373839). In a recent study comparing a combined technique of multiparametric MRI (mpMRI) with ¹⁸F-fluorocholine PET, average serum level of PSA was 1.1 ng/mL in group A and 1.9 ng/mL in group B, retrospectively (40). PSMA PET, whereas, can detect BCR at a very lower level of PSA. The tumor detection rates ranged wide from the 50.0% to 89.5%, according to the PSA levels set by retrospective studies (4142). An overall detection rate was 89.5% in a retrospective series study for 248 patients after RP, and detection rates for early BCR differed by PSA levels; 57.9% at PSA levels of 0.2–0.5 ng/mL, and 72.7% at PSA levels of 0.5–1.0 ng/mL, respectively (41). The detection rates of ⁶⁸Ga-PSMA PET for tumor recurrence gradually increased according to the PSA levels (Fig. 2) (42). In a recent meta-analysis analyzing 16 articles including 1309 patients, the pooled detection rate was 58% at PSA levels of 0.2–1.0 ng/mL, which increased to 76% with PSA levels of 1.0–2.0 ng/mL and further increased to 95% for PSA > 2.0 ng/mL (42). However, the cut-off value for PSA performing PSMA PET has yet to be concluded, and thus prospective studies are required to recommend PSMA PET for patients with BCR.

Recently, a few studies raised possibilities that the detection rates of PSMA PET are also related with PSA doubling time. Aforementioned retrospective studies failed to prove the association between the detection rates and PSA doubling time in patients with BCR (2341). However, in a recent study analyzing 39 patients with a PSA level of less than 2 ng/mL, a strong relationship was found between the detection rates and PSA doubling time; the detection rates were 85% for a PSA doubling time of less than 6.5 months but only 19% for a PSA doubling time of greater than 6.5 months (43). In addition, higher sensitivities were noted in patients with shorter PSA doubling times and those with higher initial Gleason scores (44). Interpretation should be cautiously applied for both studies, since reliabilities are limited because of small number of study populations. Further studies with a larger number of patients are required to prove this relationship between the detection rates and PSA doubling time.

Potential roles of PSMA PET have yet to be fully investigated for other clinical applications, except patients with BCR after curative treatments. Recently, it has been suggested that PSMA PET is useful for primary staging in patients with high-risk PCa. Almost one third of PCa patients will experience a BCR within 10 years after primary treatment. Thus, it is of great concern for many clinicians to distinguish PCa with a malignant potential that will eventually recur despite successful local treatments, from other type of localized PCa. Accurate initial staging in consideration of malignant potential is one of the clinical unmet needs in the management of PCa, since it is a requisite for tailoring initial and subsequent treatment strategies. The diagnostic performances of ⁶⁸Ga-PSMA PET for primary staging are yet to be proven, but several studies have shown promising results.

Primary staging is important to establish treatment strategies in PCa patients with high-risk disease before surgical procedures or external beam radiation therapy (Fig. 3). In particular, LN and bone metastases are likely to be present in patients with high-risk disease (Gleason score > 7, PSA > 20 ng/mL, clinical stage T2c-3a), even at the initial presentation. Although guidelines recommend to perform abdominal CT and bone scan for the evaluation of metastases in high-risk patients, diagnostic performances of these modalities are still poor (45). In patients with lower PSA levels, it is particularly difficult to detect metastases by conventional imaging modalities. The diagnostic accuracies for the detection of bone and LN metastases were associated with PSA levels in a meta-analysis evaluating 23 studies; 2.3% for PSA < 10 ng/mL, 5.3% for PSA 10.1–19.9 ng/mL, and 16.4% for PSA 20.0–49.9 ng/mL, respectively (12). The imaging modalities in the diagnosis of bone metastases were compared in a meta-analysis evaluating 27 studies, the pooled sensitivity and pooled specificity of bone scan with ^99mTc-bisophophonate were 79% and 82%, respectively (46). The diagnostic accuracy of CT and MRI for the pelvic LN staging are so poor that the pooled sensitivity was 42% (95% confidential interval: 26–56%), and the pooled specificity was 82% (95% confidential interval: 80–83%), respectively, in a meta-analysis evaluating 24 studies (47).

Several studies have demonstrated the superiority of PSMA PET compared to conventional imaging modalities for the detection of LN metastases at primary staging. In a patient with high-risk disease, staging may be beneficial to provide a curative intent treatment plan, but the preoperative evaluation of LN metastasis by conventional imaging modalities has limited sensitivity and specificity. Conventional imaging modalities apply only size criteria to determine LN metastasis; pelvic LNs larger than 8 mm to 10 mm in diameter are usually considered suspicious (47). The sensitivity of CT and MRI in the LN detection was found to be < 40% using minimum size of 10 mm as a threshold, and about 80% of the pathologically-proven metastatic LNs were smaller than 8 mm (48). The sensitivity of morphological cross-sectional imaging was low without significant difference in the diagnostic performance between CT and MR (47). Therefore, pelvic LN dissection or sampling is considered to be the gold standard for the LN staging in PCa patients (4950).

In a meta-analysis analyzing ⁶⁸Ga-PSMA PET, the pooled sensitivity for the LN detection was 61% and pooled specificity was 97% by patient-based analysis (42). Similar results were deducted by a prospective study performed in 30 patients with intermediate-to high-risk PCa using ⁶⁸Ga-PSMA PET; sensitivity and specificity for the LN detection were 64% and 95%, respectively by per-patient analysis (51). In this study, the size dependence of positively imaged LN was suggested as the median size of metastatic deposit of true-positive LNs was significant larger than that of false-negative LNs (4.7 mm vs. 2.7 mm) in the histopathological evaluation. It is understandable considering of the spatial resolution of state-of-the-art PET cameras with 4.9 mm and 5.1 mm (52). Also, a close association was suggested between detection rates of the LN and the size of LNs. The median size of detected LNs was significantly larger than that of undetected LNs (13.6 mm vs. 4.3 mm) in a study using ⁶⁸Ga-PSMA PET for the evaluation of patients with high-risk disease prior to RP (53). The detection of radiologically occult LN metastases can significantly influence patient management by modification of treatment plans, but the impact of improved sensitivity by ⁶⁸Ga-PSMA PET on overall survival remains unanswered in patients with high-risk disease.

Prostate-specific membrane antigen PET is also expected to demonstrate a better diagnostic accuracy for the detection of bone metastases in primary staging. A comparison study was retrospectively performed in 126 patients, which revealed that a sensitivity and specificity of 98.8–99.0% and 98.9–100% for ⁶⁸Ga-PSMA PET, and 82.4–86.6% and 91.6–97.9% for bone scan, respectively, by lesion-based analysis (20). In this study, ⁶⁸Ga-PSMA PET showed better results in all subgroup analyses, except patient-based analysis in castration-resistant PCa (CRPCa). This PSMA finding suggested a loss of PSMA expression that is infrequently occurred in CRPCa patients (54). It seems relevant that bone scan or ¹⁸F-NaF PET are followed in order to assess PSMA-negative tumors or sclerotic bone lesions on PSMA PET.

However, the application of PSMA PET is limited for the evaluation of primary prostate tumor, because the spatial resolution of PET is relatively low and exact evaluation of the prostate area can be hampered by the retention of the PET tracer in the bladder. Combination with multi-parametric magnetic resonance imaging (mpMRI) or development of novel PSMA ligands with different biodistribution profile are suggested to enhance diagnostic accuracies of PSMA PET of primary tumor. In a retrospective study comparing ⁶⁸Ga-PSMA PET and mpMRI, ⁶⁸Ga-PSMA PET was well correlated with the tumor extension assessed in mpMRI in patients with a high pre-test probability for large primary tumor in the primary staging (55). Furthermore, simultaneous acquisition of ⁶⁸Ga-PSMA PET with mpMRI was reported to improve the localization of primary tumor, and ⁶⁸Ga-PSMA PET was more accurate in the primary tumor localization, as compared with mpMRI (56). Further details about the combination with PET and MRI will follow in the later section of this review.

Imaging guided targeted biopsy can be a possible application of PSMA PET at the initiation presentation. High false-negative results are inherent from the biopsy method and tumor heterogeneity of PCa, and targeted biopsy after negative result is often recommended to patients with high suspicion of PCa. Recently, a supplementary role of ⁶⁸Ga-PSMA PET was suggested for the guidance of repeated biopsies in patients with high suspicion of PCa (56). In addition, ⁶⁸Ga-PSMA PET-guided biopsy could be used for the surveillance of patients who already underwent multiple repeated biopsies. For this purpose, combination of ⁶⁸Ga-PSMA PET with mpMRI was investigated to allow for the potential image-guided fusion biopsy incorporating information provided by mpMRI, which could lead to an increase of the diagnostic confidence (56).

Prostate-specific membrane antigen PET can assist salvage radiotherapy (SRT) by accurately delineating the target tumor volume. SRT is the main therapeutic option for patients with BCR after RP, which may potentially be curative or enhance the probability of progression-free survival (5758). It is essential to delineate clinical target volumes (CTVs) so as to include all the areas with potential microscopic occult tumors in SRT planning. The potential roles of PSMA PET have been investigated in several retrospective studies (5859), which is limited because of inhomogeneous study populations and study designs. Recently, a multicenter post hoc retrospective analysis of ⁶⁸Ga-PSMA-11 PET was performed in 270 patients with early BCR at very low PSA levels < 1.0 ng/mL (60). It was reported that there was a major impact on SRT planning, which suggests the presence of at least one PSMA-positive lesion that was originally not covered by the consensus CTVs. However, it still remains unclear that expansion of radiation field could be beneficial in patients with BCR, putting up with additional toxicity. Further studies including randomized trials of SRT are required to evaluate potential benefits of PSMA PET in SRT planning.

Prostate-specific membrane antigen PET can be utilized in monitoring treatment response of PCa. Although Response Criteria in Solid Tumors (RECIST) guideline is commonly used for the response evaluation, revised RECIST 1.1 application is limited for the evaluation of PCa because of the high prevalence of non-measurable lesions in the LNs and bones. Currently, no reliable, objective surrogate marker is available for the response evaluation of metastatic PCa, considering that bone is the most frequently and almost exclusively metastasized sites. The diagnostic accuracies of bone scan or ¹⁸F-NaF PET for the response evaluation can be hampered by flare phenomenon. In this regard, PSMA PET can be a potential candidate for the monitoring of systemic disease. PET Response Criteria in Solid Tumors (PERCIST) was initially developed for systematic and structured assessment of response to therapy with ¹⁸F-fluorodeoxyglucose (61). In order to apply PSMA PET for the response evaluation of PCa, validation studies are required using PERCIST in PCa patients.

Another promising application of PSMA PET is the realization of theranosis. Therapeutics using PSMA ligands labeled with therapeutic radionuclides (¹⁷⁷Lu or ⁹⁰Y) has been developed for patients with advanced and metastatic PCa. Although PSMA-11 is the most widely investigated PSMA ligand for PET, but it is not available for RLT. PSMA-11 is unlikely to bind with other radiometals except ⁶⁸Ga. Instead of PSMA-11, PSMA-617 labeled with a therapeutic radionuclide was developed for PSMA RLT (62). ¹⁷⁷Lu-PSMA-617 is the most actively investigated radioligand for the treatment of PCa, and it has yielded promising outcomes in a multinational multicenter clinical trial in a large cohort of PCa patients (63). Accordingly, there is a growing experience regarding theranostic applications of PMSA PET. Multiple sessions of PSMA PET performed before and during PSMA RLT. In particular, PSMA PET is required for the patient selection before PSMA RLT, since it is crucial to determine the presence and intensity of PSMA expression in PCa patients (63646566). PSMA RLT can be futile, leaving immense complications without significant clinical benefits when given to patients presenting with a low level of PSMA expression.

There are several studies comparing ⁶⁸Ga-PSMA PET to choline PET; however, data from prospective multicenter trials are not yet available. In a retrospective study evaluating 67 patients with 458 LN metastases, ⁶⁸Ga-PSMA-11 demonstrated a higher detection rate in patients with BCR than that of ¹¹C-choline (67); 39% were exclusively identified with ⁶⁸Ga-PSMA-11, while 6% were identified with ¹¹C-choline. There was a study comparing ¹⁸F-fluorocholine and ⁶⁸Ga-PSMA PET, and these two PET scans performed within 30 days in 37 patients with BCR; 86.5% of tumors were detected by ⁶⁸Ga-PSMA-11, and 70.3% were detected by ¹⁸F-fluorocholine by a patient-based analysis (26). In this study, ⁶⁸Ga-PSMA-11 offered a higher detection rate, higher maximum standardized uptake value, and higher tumor-to-background ratio than ¹⁸F-fluorocholine. In another study with a prospective design demonstrated that ⁶⁸Ga-PSMA exhibited a better diagnostic accuracy than did ¹⁸F-fluorocholine in patients with BCR (17).

There are several different PSMA ligands for ⁶⁸Ga complexation; ⁶⁸Ga-PSMA-11, ⁶⁸Ga-PSMA-617, and ⁶⁸Ga-PSMA-I&T. These PSMA ligands are collectively known as ⁶⁸Ga-PSMA in this review, because they shared similar biodistribution and imaging properties. Studies discussed in the above section were mainly performed with ⁶⁸Ga-PSMA-11 PET, but only a small number of studies have been performed using other ⁶⁸Ga-PSMA ligands, and the difference in the diagnostic performances of ⁶⁸Ga-PSMA ligands appears marginal. A retrospective study with ⁶⁸Ga-PSMA-I&T reported detection rates of 52% for a PSA level of < 0.5 ng/mL, 55% for 0.5–1.0 ng/mL, 70% for 1.0–2.0 ng/mL, and 93% for 2.0–5.0 ng/mL; these results were relatively comparable to those for ⁶⁸Ga-PSMA-11 (68). Notably, there are still no data directly comparing the ⁶⁸Ga-PSMA ligands each other, and ⁶⁸Ga-PSMA-11 is the most prominent PSMA ligand for PET imaging in PCa. The most suitable PSMA ligand for both diagnosis and treatment is yet to be determined, and investigations continue to accomplish an efficient and successful theranosis for PCa.

In general, ¹⁸F-based PET is known to be advantageous over ⁶⁸Ga-based PET, but there are no head-to-head comparison studies comparing ⁶⁸Ga-PSMA and ¹⁸F-PSMA PET. In a study evaluating 14 selected patients who underwent both ¹⁸F-DCFPyL PET and ⁶⁸Ga-PSMA-11 PET, ¹⁸F-DCFPyL PET revealed additional lesions in 3 out of 14 patients in whom ⁶⁸Ga-PSMA-11 PET revealed negative and inconclusive findings (69). In a follow-up study performed by the same group, PSA-stratified detection rates were compared in different patients and the diagnostic accuracy of ¹⁸F-DCFPyL PET was non-inferior to that of ⁶⁸Ga-PSMA-11 PET (70). These initial data suggested that ¹⁸F-PSMA PET might have improved sensitivity for the detection of relapsed tumor in patients with BCR after RP with moderately increased PSA levels. However, these data should be interpreted with caution because of the different patient populations, administered activities, and PET acquisition techniques. Further studies are required to confirm the potential superiority of ¹⁸F-PSMA PET over other imaging modalities.

MRI is considered to be the standard imaging tool for the evaluation of soft tissues, but MRI remains challenging for the diagnosis of PCa. mpMRI is accurate and useful to evaluate PCa, since it combines conventional anatomical imaging based on T2-weighted sequences and functional imaging such as diffuse-weighted sequences. mpMRI has been integrated into the management of PCa, in particular for assisting prostate biopsies through image guidance. It has been demonstrated that mpMRI-guided prostate biopsies enhanced diagnostic yields for patients with clinically significant PCa, which lead to improvement of patient care (7172). However, the reported diagnostic performances of T2-weighted sequences varied widely in the detection of PCa, with sensitivities of 47.8–88.2% and specificities of 44.3–81.0%, respectively (73). Treatment-induced changes such as distorted anatomy, fibrosis, artifacts can contribute misinterpretations of MRI findings, and thus hinder the diagnostic accuracy. In addition, the widespread use of mpMRI has been hampered by its relatively high cost, its complexity, and lack of training.

Recently, technological progress including simultaneous acquisition of PET and MRI (hybrid PET/MRI) has lead the active use of PET/MRI. Functional information provided by PET is expected to enhance diagnostic performances of conventional anatomical MRI. The hybrid PET/MRI allows optimal conditions for the registration of the two modalities, and thus both PET and MRI data can be reliably acquired in a single session. Moreover, the hybrid PET/MRI enables the whole-body survey in one examination without additional radiation originating from the use of CT. In this respect, the hybrid PET/MRI provides a potential added value of combined MRI and PET over mpMRI alone or PET/CT.

The feasibility of the hybrid PET/MRI has been investigated with various prostate specific PET tracers. The diagnostic benefits of the hybrid PET/MRI with the clinically established choline tracers were suggested in the detection of PCa (7475). The diagnostic performances of the hybrid PET/MR with ¹⁸F-fluorocholine and ¹¹C-choline was compared with that of mpMRI alone, which showed a better diagnostic accuracy in the peripheral zone than in the transition zone of the prostate (75). PSMA-targeting PET tracers have also been explored for the implementation of the hybrid PET/MRI. In the diagnosis of recurrent PCa, ⁶⁸Ga-PSMA PET/MRI detected PCa more easily and more accurately than ⁶⁸Ga-PSMA PET/CT, and unclear findings on PET/CT could be clarified by PET/MRI (76). Also, ⁶⁸Ga-PSMA PET/MRI improved the localization of primary PCa, compared both with mpMRI and with PET alone (56). Recently, ¹⁸F-PSMA-1007 was applied for the hybrid PET/MRI system in PCa patients (77). In this study, ¹⁸F-PSMA-1007 presented better imaging features compared with those of ⁶⁸Ga-PSMA, which is mainly due to improved pharmacokinetics of ¹⁸F-PSMA-1007 and optimized imaging protocols for the hybrid PET/MRI.

The hybrid PET/MRI with prostate specific PET tracers is a promising tool to add potential values in the diagnosis of PCa, and it is also expected to significantly influence on the management of PCa patients. However, further technological advances including optimization of imaging protocols and selection of the best suited PET tracer are needed to become a preferred imaging tool for PCa. In addition, prospective studies using the hybrid PET/MRI with prostate specific PET tracers are required, particularly in comparison with other imaging modalities such as PET/CT and mpMRI alone.