Globicatella sanguinis Bacteremia in a Korean Patient

Kwangjin Ahn; Gyu Yel Hwang; Kap Jun Yoon; Young Uh

doi:10.5145/ACM.2018.21.2.40

Journal List > Ann Clin Microbiol > v.21(2) > 1096958

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Ahn, Hwang, Yoon, and Uh: Globicatella sanguinis Bacteremia in a Korean Patient

Original Article

Ann Clin Microbiol 2018;21(2):40-44.

Published online: 12 June 2018

DOI: https://doi.org/10.5145/ACM.2018.21.2.40

Globicatella sanguinis Bacteremia in a Korean Patient

Kwangjin Ahn, Gyu Yel Hwang, Kap Jun Yoon, Young Uh

^¹Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, Korea

Correspondence: Young Uh, Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju 26426, Korea. (Tel) 82-33-741-1592, (Fax) 82-33-731-0506, (E-mail) u931018@yonsei.ac.kr

Received 23 January 2018 Revised 7 March 2018 Accepted 19 March 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Globicatella sanguinis is an unusual pathogen causing bacteremia, meningitis, and urinary tract infection, and can be misidentified as Streptococcus pneumoniae or viridans streptococci due to its colonial morphology. A 76-year-old female patient with hypertension and degenerative arthritis was admitted to the hospital complaining of knee joint pain. Blood culture revealed the presence of Gram-positive cocci, and the isolated organism was equally identified as S. pneumoniae using the MicroScan identification system (Beckman Coulter, USA) and Vitek 2 identification system (bioMérieux, USA). However, the isolate showed optochin resistance based on the optochin disk susceptibility test. The organism was finally confirmed to be G. sanguinis based on 16S rRNA sequencing and hydrogen sulfide production testing. Accurate identification of G. sanguinis isolated from aseptic body fluids including blood is important for appropriate antibiotic selection based on accurate application of interpretative criteria of antimicrobial susceptibility test.

Keywords: Bacteremia, Globicatella sanguinis, 16S rRNA sequencing

References

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Fig. 1.

Neighbor joining phylogenetic tree showing the relationship of our case to Globicatella species. The black dotted G. sanguinis is our isolate. Species name and accession code are given as cited in the EzBioCloud database.

Table 1.

Phenotypic characteristics of our isolate identified by commercial kits and Globicatella species previously reported

Characteristics	Our isolate*	G. sanguinis			G. sulfidaciens
		Previous study by			Previous study by Vandamme et al. [4]
		Facklam [3]^†	Shewmaker et al. [2]^†	Collins et al. [1]	Previous study by Vandamme et al. [4]
Leucine aminopeptidase	ND	−	−	ND	ND
Pyrrolidonylarylamidase	ND	V	V	+	−
Growth in 6.5% NaCl	−	+	+	ND	+
Bile-esculin reaction	−	+	ND	ND	ND
Esculin hydrolysis	ND	+	+	ND	+
Voges-Proskauer reaction	−	−	ND	ND	−
H₂ S production	−	ND	ND	−	+
β-galactosidase	+	ND	ND	+	−
β-glucuronidase	−	ND	ND	−	+
Hippurate hydrolysis	ND	+	+	+	−
Optochin resistance	−	ND	ND	ND	ND
Acidification
Arginine	−	−	ND	ND	ND
Mannitol	−	+	+	+	−
Sorbitol	−	V	V	ND	−
Arabinose	−	ND	V	ND	−
Lactose	−	ND	+	ND	−
Maltose	+	ND	+	ND	+
Sucrose	−	ND	+	ND	+
Inulin	−	ND	ND	−	+
Ribose	−	ND	ND	+	−

*+, positive; −, negative; ^†+, positive reactions ≥92%; −, positive reactions ≤8%; v, variable reactions positive in 9 to 91% of strains; ^‡+, positive reactions ≥85%; −, positive reactions ≤15%; v, variable reactions positive in 16 to 84% of strains.

Abbreviation: ND, not done.

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