Journal List > Korean J Pediatr Infect Dis > v.19(2) > 1096069

Lee, Choi, Kim, Ahn, Choi, Ma, Park, Kim, and Kang: Immunoassay of Pertussis According to Ages

Abstract

Purpose

We conducted the immunoassay of pertussis according to ages, in order to evaluate protective immunity against pertussis in Korean populations.

Methods

Healthy subjects were enrolled at four university hospitals in Korea. The subjects were grouped as seven age groups (every 10 years). Antibodies against pertussis toxin (PT) in sera were measured by enzyme linked immunosorbent assay (ELISA) kits. Geometric mean concentrations (GMC) of antibodies and the ratios of the subjects with seroprotective antibody levels were determined. The subjects with antibody titers ≥24.0 EU/mL were considered to seroprotective as the manufacturer's protocol.

Results

Total 1,605 subjects (age: 2 months-65 years) participated in this study, and their GMC was 56.16±50.54 EU/mL. Among seven age groups, age group <11 year showed the highest GMC (64.78±53.24 EU/mL) (P<0.001). In the analysis of the ratios of the subjects with seroprotective antibody titers, 68.2% of the subjects were proven to seroprotective, and age group <11 year also showed the highest ratio (76.5%) (P<0.001).

Conclusions

We found that adolescences or adults (age group ≥11 year) showed lower levels of antibody against pertussis and lower ratio of the subjects with seroprotective antibody titers than children (age group <11 year).

Figures and Tables

Fig. 1
Geometric mean concentrations (GMC) of subjects' antibodies against pertussis toxin (PT) are shown by age group and sex. (A) Adolescents and adults, and (B) children. Data of the past study performed in 200214) are also shown to compare the differences.
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Table 1
Study Subjects and Anti-Pertussis Toxin (PT) Antibody Concentrations by Age Group
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*Seroprotective antibody concentrations

Abbreviations: GMC, geometric mean concentration; SD, standard deviation

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