Prevalence of Viruses with Diarrhea among Hospitalized Children West Gyeonggi Province

Soo Young Seo; In Ah Jung; Ji Hoon Kim; Kyung Soon Cho; Joong Hyun Bin; Hyun Hee Kim; Hee Jin Lee; Wonbae Lee

doi:10.14776/kjpid.2012.19.1.28

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Seo, Jung, Kim, Cho, Bin, Kim, Lee, and Lee: Prevalence of Viruses with Diarrhea among Hospitalized Children West Gyeonggi Province

Original Article

Korean Journal of Pediatric Infectious Diseases

Korean Journal of Pediatric Infectious Diseases 2012; 19(1): 28-36.

Published online: 30 April 2012

DOI: https://doi.org/10.14776/kjpid.2012.19.1.28

Prevalence of Viruses with Diarrhea among Hospitalized Children West Gyeonggi Province

Soo Young Seo, M.D., In Ah Jung, M.D., Ji Hoon Kim, M.D., Kyung Soon Cho, M.D., Joong Hyun Bin, M.D., Hyun Hee Kim, M.D., Hee Jin Lee^*, Wonbae Lee, M.D.

Department of Pediatrics, Bucheon St Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

^*Clinical Medical Research Institute, Bucheon St Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Correspondence: Wonbae Lee, M.D. Department of Pediatrics, Bucheon St Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. Tel: +82-32-340-7045, Fax: +82-32-340-2673, leewb@catholic.ac.kr

Received 21 October 2011 Revised 25 October 2011 Accepted 25 October 2011

Abstract

Purpose

This study was conducted to evaluate epidemiological data of the viral pathogens obtained from stool exams and provide information on the regional prevalence of infectious diarrheal disease west in Gyeonggi Province, Korea.

Methods

We enrolled a cohort of children <10 years of age admitted for treatment of acute diarrhea at Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea. In total, 310 fecal specimens, documented to be free of common bacterial pathogens, were collected from pediatric patients during a 12-month period from January to December 2009 and were tested for the presence of rotavirus, parechovirus, adenovirus, astrovirus, enterovirus, and norovirus using polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) assay.

Results

The most common virus was parechovirus (16%), followed by adenovirus (15%), astrovirus (14%), rotavirus (13%), and enterovirus (5%). Interestingly, only one of the specimens was positive for norovirus. Single infection cases were detected in 173 (55.8%) of the 310 children, whereas mixed viral infections were detected in 10 (3.2%) of the same children. Viral gastroenteritis generally showed a double peak of incidence. Parechovirus, rotavirus, and adenovirus shared a similar pattern of peak incidence with overall viruses; however, astrovirus infections occurred more frequently in the spring. Eighty-five percent of the confirmed viral gastroenteritis cases developed in under 24 months.

Conclusion

The results support the importance of parechovirus, adenovirus, astrovirus, and enterovirus as causative agents of diarrhea in children, which may be underestimated by current routine diagnostic testing.

Keywords: Parechovirus, Adenovirus, Astrovirus, Rotavirus, Enterovirus, Norovirus

Figures and Tables

Fig. 1

Distribution of rotavirus-, adenovirus-, astrovirus-, norovirus-, enterovirus-, and parechovirus-positive detection by PCR and RT-PCR among 183 stool samples from hospitalized children in Korea. Distribution (%) of the total number of single (n=173) and mixed (n=10) viral intestinal infections among 310 stool samples from hospitalized children. We identified 55.8% (173/310) of single infections and 3.2% (10/310) of multiple infections.

Fig. 2

Monthly distribution of sporadic gastroenteritis cases during 2009.

Table 1

Primers Used for PCR and RT-PCR

Table 2

Infecting Virus and Relationship to Gender, Age, and Hospitalization

Table 3

Absolute Frequency of Infection with Different Viruses in Different Age Groups

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