Serum visfatin levels in non-obese women with polycystic ovary syndrome and matched controls

Jin Ju Kim; Young Min Choi; Min A Hong; Min Jeong Kim; Soo Jin Chae; Sun Mie Kim; Kyu Ri Hwang; Sang Ho Yoon; Seung Yup Ku; Chang Suk Suh; Seok Hyun Kim

doi:10.5468/ogs.2018.61.2.253

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Kim, Choi, Hong, Kim, Chae, Kim, Hwang, Yoon, Ku, Suh, and Kim: Serum visfatin levels in non-obese women with polycystic ovary syndrome and matched controls

Original Article

Reproductive Endocrinology

Obstetrics & Gynecology Science 2018; 61(2): 253-260.

Published online: 5 February 2018

DOI: https://doi.org/10.5468/ogs.2018.61.2.253

Serum visfatin levels in non-obese women with polycystic ovary syndrome and matched controls

Jin Ju Kim^1,², Young Min Choi^2,³

, Min A Hong³, Min Jeong Kim¹, Soo Jin Chae⁴, Sun Mie Kim¹, Kyu Ri Hwang⁵, Sang Ho Yoon⁶, Seung Yup Ku^2,³, Chang Suk Suh^2,³, Seok Hyun Kim^2,³

¹Department of Obstetrics and Gynecology, Healthcare System Gangnam Center, Seoul National University Hospital, Seoul, Korea.

²The Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.

³Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.

⁴Department of Obstetrics and Gynecology, Maria Fertility Hospital, Seoul, Korea.

⁵Department of Obstetrics and Gynecology, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Seoul, Korea.

⁶Department of Obstetrics and Gynecology, Graduate School of Medicine, Dongguk University, Seoul, Korea.

Corresponding author: Young Min Choi. Department of Obstetrics and Gynecology, The Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, Seoul 03080, Korea. ymchoi@snu.ac.kr

Received 24 May 2017 Revised 30 August 2017 Accepted 6 September 2017

Korean Society of Obstetrics and Gynecology

(open-access):

Articles published in Obstet Gynecol Sci are open-access, distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective

The purpose of the current study was to compare the circulating levels of visfatin between women with polycystic ovary syndrome (PCOS) and those without PCOS and to assess the correlations between visfatin levels and various parameters.

Methods

This case-control study recruited 74 PCOS patients and 74 age- and body mass index (BMI)-matched controls. Serum visfatin levels were evaluated using the enzyme-linked immunosorbent assay. Women with PCOS were divided into 2 subgroups based on the presence of clinical or biochemical hyperandrogenism. The possible differences in serum visfatin levels between the hyperandrogenic and non-hyperandrogenic groups were also assessed.

Results

Visfatin levels in PCOS patients were similar to those in the controls. However, hyperandrogenic patients had significantly higher mean serum visfatin levels than those in non-hyperandrogenic patients (3.87 ng/mL; 95% confidence intervals [CIs], 3.09–4.85 in hyperandrogenic group vs. 2.69 ng/mL; 95% CIs, 2.06–3.52 in non-hyperandrogenic group; P=0.038). In women with PCOS, visfatin levels positively correlated with BMI (r=0.23; P=0.047) and the log free androgen index (FAI) (r=0.27; P=0.021) and negatively correlated with high-density lipoprotein (HDL) cholesterol levels (r=−0.37; P=0.025). Except for HDL cholesterol levels, these correlations were also observed in controls.

Conclusion

Visfatin levels in PCOS patients were similar to those in the controls. However, hyperandrogenic patients showed significantly higher serum visfatin levels than those of non-hyperandrogenic patients, and visfatin had a positive linear correlation with FAI in both PCOS patients and controls.

Keywords: Adipocytokine, Polycystic ovary syndrome, Nicotinamide phosphoribosyltransferase, Visfatin

Introduction

Polycystic ovary syndrome (PCOS), which is characterized by chronic anovulation and hyperandrogenism, is a common endocrine disorder in women of reproductive age. Insulin resistance (IR) is one of the core pathophysiological characteristics of this syndrome. Thus, besides its association with reproductive morbidity, PCOS is known as a metabolic disorder. Women with PCOS are more predisposed to hypertension and dyslipidemia and have an excess risk of type 2 diabetes and subclinical atherosclerosis [1 2]. Obesity is also common (20%–70%) in women with PCOS.

Visfatin is a new adipokine found in subcutaneous, visceral, perivascular, and epicardial fat tissues [3 4 5 6]. Serum visfatin levels correlate well with body mass index (BMI) or percentage of body fat [3] and are higher in obese subjects than those in controls [3 7]. At first, visfatin was considered to mainly have insulin-mimetic properties such as stimulating glucose uptake in adipocytes and suppressing glucose release from hepatocytes in vitro [8]. However, subsequent studies have found connections between visfatin and inflammation, endothelial dysfunction and atherosclerosis; these connections suggest a possible role of visfatin as a biomarker of low-grade inflammation and metabolic complications [9 10 11 12 13]. Curat et al. [14] reported that visfatin is not only synthesized by adipocytes but also by inflammatory cells such as macrophages in adipose tissue, suggesting that visfatin might be a proinflammatory marker.

PCOS is associated with the dysfunctional secretion of adipokines, promoting inflammation and IR [15 16]. Substantial numbers of studies have reported that the gene expression or serum levels of visfatin are significantly higher in women with PCOS than those in matched controls [4 17 18 19 20 21 22]. Pepene [23] reported that visfatin is an independent predictor of endothelial dysfunction in women with PCOS. However, some studies have reported no differences in serum visfatin levels between PCOS patients and controls [24 25 26 27].

The purpose of the current study was to compare circulating visfatin levels in women with PCOS to those in women without PCOS and to assess the correlations between visfatin and various parameters. Considering that higher visfatin levels have been reported in hirsute PCOS patients and that positive correlations between serum visfatin and androgen levels have been reported [19 23 28], this study also assessed whether serum visfatin levels differ between hyperandrogenic and normo-androgenic PCOS patients. To minimize the effect of obesity, the study was performed in non-obese women with PCOS and age- and BMI-matched controls.

Materials and methods

1. Subjects

For this study, data from a previously described cohort were analyzed. The detailed diagnostic process is described in previous studies [29 30 31]. Briefly, a total of 74 premenopausal women were enrolled as PCOS patients, and a diagnosis was based on the 2003 Rotterdam consensus meeting guideline [32]. Oligomenorrhea was defined as less than 8 periods per year or cycles longer than 35 days, and amenorrhea was defined as the absence of menstruation for more than 3 months without pregnancy. Clinical hyperandrogenism was defined by a modified Ferriman and Gallwey score (mF-G score) of 6 or greater [30]. Biochemical hyperandrogenism was defined as total testosterone (T) >0.68 ng/mL, free T >1.72 pg/mL, or free androgen index (FAI) >5.36 [29].

A total of 74 premenopausal women were matched with patients based on age (±3 year) and BMI (±1 kg/m²). Matching was performed randomly without replacement at a ratio of 1:1. Including criteria for controls has also been previously described [31]. Neither the cases nor the controls had taken combined oral contraceptives, lipid-lowering agents, insulin sensitizer or anti-androgens or had a history of diagnosed diabetes. The review board for human research of the Seoul National University Hospital approved this project (Institutional Review Board [IRB] No. 0807-031-250), and written informed consent was obtained from all subjects.

2. Clinical and biochemical measurements

Clinical variables such as body weight, height, waist circumference (WC) and blood pressure (BP) were assessed, and BMI was calculated as weight (kg) divided by the square of height (m²). Subjects who had a BMI less than 25 kg/m² were enrolled according to the definition of obesity for Asians [33].

All PCOS patients were evaluated for serum luteinizing hormone, follicle stimulating hormone and estradiol, total T, free T, 17-hydroxyprogesterone and sex hormone binding globulin (SHBG) using radioimmunoassay (Simens, Los Angeles, CA, USA). FAI was calculated as (total T/SHBG)×100, and the values for T were converted from ng/mL to nmol/L using the following index provided by the manufacturer: 1 ng/mL=3.467 nmol/L. Fasting and 2-hour serum glucose and insulin levels were evaluated by a 75 g oral glucose tolerance test (OGTT) in women with PCOS. The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as glucose (mg/dL)×insulin (μU/mL)/405. Serum cholesterol, triglyceride (TG), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol and hemoglobin A1c levels were also measured in women with PCOS. In the controls, serum total T, free T, SHBG, fasting glucose and insulin, hemoglobin A1c, total cholesterol, TG, HDL cholesterol and LDL cholesterol levels were evaluated. Serum visfatin was evaluated by human visfatin enzyme-linked immunosorbent assay (AdipoGen, Liestal, Switzerland), and the intra- and inter-assay coefficients of variation were <10% and <8%, respectively.

3. Statistical analysis

Possible deviations from the normality of the data distribution were tested using visual inspection of quantile-normal plots and/or the Shapiro-Wilk test of normality. If variables did not follow Gaussian distributions, normal distribution was achieved by natural logarithmic or square root transformations, then the data were shown as geometric means with 95% confidence intervals (CIs). Hormonal and other metabolic parameters were compared using a Student's t-test. To assess the correlations between serum visfatin levels and each parameter, Pearson's correlation analysis was used. All data analyses were performed using the Statistical Package for the Social Sciences software (version 22.0; IBM SPSS, Armonk, NY, USA), and statistical significance was accepted for 2-sided P-values <0.05.

Results

The clinical and endocrine characteristics are shown in Table 1. By definition, there were significant differences in the hirsutism score and serum androgen levels between women with PCOS and the controls. There were also significant differences in systolic and diastolic BP, fasting glucose, fasting insulin, HOMA-IR, and uric acid levels between the 2 groups. However, serum lipid, hemoglobin A1c, and visfatin levels were similar between the 2 groups.

Table 1

Clinical and biochemical features of non-obese women with polycystic ovary syndrome (PCOS) matched controls

Parameters		PCOS (n=74)	Controls (n=74)	P-value
Anthropometric parameters
	Age (yr)	23.8±5.5	24.4±5.0	0.461
	BMI (kg/m²)	20.4±2.1	20.4±2.1	0.950
	WC (cm)	73.9±5.5	75.5±6.6	0.256
	Hirsutism score	5 (0–9)	0 (0–3)	<0.001
	Systolic BP (mmHg)	113.5±11.9	105.7±9.9	<0.001
	Diastolic BP (mmHg)	75.3±6.5	64.3±6.3	<0.001
Hormonal parameters
	Total testosterone (ng/mL)	0.37 (0.33–0.42)	0.22 (0.18–0.29)	<0.001
	Free testosterone (pg/mL)	0.91 (0.80–1.02)	0.36 (0.27–0.47)	<0.001
	SHBG (nmol/L)	49.0 (43.1–55.7)	54.9 (44.8–67.2)	0.401
	FAI	3.4 (2.2–4.6)	1.4 (1.1–1.7)	<0.001
	Luteinizing hormone (IU/L)	7.6 (6.8–8.4)	Not checked	-
	Follicle stimulating hormone (IU/L)	4.5 (4.2–4.8)	Not checked	-
	Estradiol (pg/mL)	61.1±10.0	Not checked	-
Metabolic parameters
	Fasting glucose (mg/dL)	87.6±7.6	84.9±6.1	0.040
	Fasting insulin	8.55 (7.55–9.69)	5.37 (4.33–6.66)	<0.001
	HOMA-IR	1.79 (1.56–2.04)	1.20 (0.98–1.47)	0.002
	75 g OGTT glucose	94.6±18.7	Not checked	-
	75 g OGTT insulin	46.3 (39.3–51.3)	Not checked	-
	Total cholesterol (mg/dL)	175.5±28.3	172.0±26.6	0.521
	Triglyceride (mg/dL)	81.5±34.3	75.1±27.4	0.307
	HDL cholesterol (mg/dL)	65.8±16.5	62.14±10.7	0.202
	LDL cholesterol (mg/dL)	94.5±22.7	94.9±21.9	0.943
	Hemoglobin A1c (%)	5.39±0.69	5.50±0.23	0.339
	Uric acid (mg/dL)	4.63±0.94	4.25±0.69	0.031
Visfatin (ng/mL)		3.41±1.41	3.28±1.01	0.789

Data are shown as the means±standard deviation, median (interquartile range), or geometric mean (95% confidence intervals). P-values are indicated for the differences between groups, as analyzed using a Student's t-test.

BMI, body mass index; WC, waist circumference; BP, blood pressure; SHBG, sex hormone binding globulin; FAI, free androgen index; HOMA-IR, homeostasis model assessment of insulin resistance; OGTT, oral glucose tolerance test; HDL, high-density lipoprotein; LDL, low-density lipoprotein.

For further analysis, women with PCOS were divided into 2 subgroups based on the presence of hyperandrogenism. If a patient has any of the clinical hyperandrogenism or biochemical hyperandrogenemia, then she was categorized as the hyperandrogenic group. Differences in serum visfatin levels between the hyperandrogenic and non-hyperandrogenic groups were assessed (Table 2). Hyperandrogenic patients had significantly higher mean hemoglobin A1c levels, but mean levels of other metabolic variables were similar between the 2 groups. Although both groups had similar BMI and WC, significantly higher serum visfatin levels were observed in hyperandrogenic patients than those in non-hyperandrogenic patients (mean level of serum visfatin was 3.87 [95% CIs, 3.09–4.85] ng/mL in hyperandrogenic patients and 2.69 [95% CIs, 2.06–3.52] ng/mL in non-hyperandrogenic patients, respectively; P=0.038).

Table 2

Clinical and biochemical features of non-obese polycystic ovary syndrome (PCOS) patients with and without hyperandrogenism

Parameters		Hyperandrogenic PCOS (n=41)	Non-hyperandrogenic PCOS (n=33)	P-value
Metabolic parameters
	Age (yr)	23.7±5.5	23.9±5.5	0.861
	BMI (kg/m²)	20.7±2.2	20.1±1.9	0.164
	WC (cm)	67.4±5.6	66.6±5.5	0.566
	Hirsutism score	6.5 (1–9)	3 (0–5)	<0.001
	Systolic BP (mmHg)	113.7±13.5	113.0±9.5	0.767
	Diastolic BP (mmHg)	75.3±6.8	75.4±6.3	0.963
	Fasting glucose (mg/dL)	84.3±6.8	85.6±5.2	0.385
	HOMA-IR	1.78 (1.48–2.16)	1.79 (1.47–2.16)	0.996
	75 g OGTT glucose	92.2±16.6	97.7±20.9	0.221
	75 g OGTT insulin	44.4 (36.6–53.9)	38.9 (30.5–49.5)	0.380
	Total cholesterol (mg/dL)	176.0±27.1	174.9±30.0	0.868
	Triglyceride (mg/dL)	83.4±38.4	79.3±29.1	0.621
	HDL cholesterol (mg/dL)	65.1±15.8	66.7±17.7	0.683
	LDL cholesterol (mg/dL)	94.3±21.4	94.8±24.5	0.929
	Hemoglobin A1c (%)	5.41±0.90	5.36±0.27	<0.001
	Uric acid (mg/dL)	4.77±1.11	4.45±0.66	0.172
Hormonal parameters
	Total testosterone (ng/mL)	0.43 (0.36–0.50)	0.32 (0.28–0.36)	0.006
	Free testosterone (pg/mL)	1.05 (0.90–1.22)	0.76 (0.64–0.91)	0.006
	SHBG (nmol/L)	44.4 (36.6–53.9)	55.3 (47.2–64.8)	0.090
	FAI	3.31 (2.55–4.30)	1.98 (1.66–2.37)	0.003
	Luteinizing hormone (IU/L)	8.4 (6.0–11.9)	8.6 (6.0–12.4)	0.978
	Follicle stimulating hormone (IU/L)	5.0 (4.1–6.0)	5.4 (4.4–6.6)	0.557
	Estradiol (pg/mL)	34.3 (24.5–47.8)	26.9 (19.2–37.7)	0.308
Visfatin (ng/mL)		3.87 (3.09–4.85)	2.69 (2.06–3.52)	0.038

BMI, body mass index; WC, waist circumference; BP, blood pressure; HOMA-IR, homeostasis model assessment of insulin resistance; OGTT, oral glucose tolerance test; HDL, high-density lipoprotein; LDL, low-density lipoprotein; SHBG, sex hormone binding globulin; FAI, free androgen index.

Finally, the correlations between serum visfatin levels and various parameters were evaluated. In women with PCOS, serum visfatin levels positively correlated with BMI (r=0.23; P=0.047) and log FAI (r=0.27; P=0.021) and negatively correlated with HDL cholesterol levels (r=−0.37; P=0.025) (Fig. 1). Except HDL cholesterol levels (r=−0.10; P=0.597), correlations between serum visfatin levels and BMI (r=0.40; P=0.021) or log FAI (r=0.27; P=0.023) were also observed in the controls. All other parameters, such as IR markers, SHBG, TG, and LDL cholesterol levels, showed no correlations with visfatin levels in both the patients and the controls (data not shown).

Fig. 1

Correlations between serum visfatin levels and various parameters in women with polycystic ovary syndrome (PCOS).

BMI, body mass index; HDL, high-density lipoprotein; FAI, free androgen index.

Discussion

The aim of the present study was to compare the circulating visfatin levels between non-obese women with PCOS and those of matched controls and to assess the correlations between visfatin levels and various parameters. According to the data of this study, serum visfatin levels were similar between PCOS patients and those of the controls. However, in women with PCOS, significantly higher serum visfatin levels were observed in the hyperandrogenic group than those in the non-hyperandrogenic group. Visfatin levels showed positive correlations with BMI and log FAI and a negative correlation with HDL cholesterol levels. Based on the knowledge that PCOS is characterized by dysfunctional secretion of adipokines promoting inflammation and IR [15 16], the increase in visfatin levels in hyperandrogenic PCOS patients or its correlation with androgen levels may suggest an association between visfatin and hyperandrogenism in women with PCOS.

Since its discovery, some association has been established between visfatin and PCOS but with conflicting results [4 17 18 19 20 21 22 23 24 25 26 27 28]. A number of studies have reported that women with PCOS have higher visfatin levels than those in BMI-matched [17 21 34 35] or unmatched controls [18 36 37 38]. Moreover, visfatin levels in normal-weight PCOS patients has been found to be higher than those in obese controls [19]. On the other hand, serum visfatin levels have been reported to be similar between women with PCOS and BMI-matched [27] or unmatched controls [24 25 26 28]. Farshchian et al. [27] reported that serum visfatin levels were higher in obese women compared to those in normal-weight subjects, but there were no differences in serum visfatin levels between PCOS patients and the controls. Thus, to minimize the effect of obesity, the current study analyzed circulating visfatin levels in non-obese patients and BMI-matched controls and found no differences in serum visfatin levels between these groups. Although there is a possibility that the small differences in serum visfatin levels in lean patients and controls may require a larger number of subjects to demonstrate differences, the findings of this study seem to indicate that visfatin levels are not directly related with the pathophysiology of PCOS after controlling for the effect of obesity.

One of the issues that needs to be addressed is that hyperandrogenic and non-hyperandrogenic PCOS patients may differ in their metabolic characteristics. In the current study, higher serum visfatin levels were observed in hyperandrogenic patients than those in non-hyperandrogenic patients, and visfatin had a positive correlation with log FAI (r=0.27; P=0.021) in women with PCOS. Gümüş et al. [28] reported that visfatin levels were similar between women with and without PCOS, but higher visfatin levels were found in hirsute adolescents with PCOS than those in non-hirsute patients. Similar to the finding of this study, some previous studies have reported that visfatin levels correlated with serum T (r=0.47; P=0.002) or FAI (r=0.48; P=0.002) in lean PCOS patients [18 19 36]. Visfatin was considered to exert insulin-mimetic properties [8], and it is well known that hyperinsulinemia can stimulate ovarian androgen synthesis. Thus, observed correlation between visfatin and FAI in the current study may be associated with insulin-like action of visfatin. Nevertheless, the current study shows no direct correlation between visfatin and the index of IR such as fasting insulin, HOMA-IR or 75 g OGTT 2-hour insulin levels. Although the findings of this study do not show a correlation between visfatin and SHBG, Panidis et al. [19] reported that plasma visfatin levels were negatively correlated with SHBG levels in normal-weight women with PCOS. There are no data regarding the direct role of visfatin in SHBG production in liver, but the correlation between visfatin and FAI may stem from the association between visfatin and SHBG. Although the current study was performed on non-obese subjects, the common influence of obesity, which might be related with both visfatin and FAI [39], cannot be completely ruled out. Further studies are required to clarify whether visfatin may be a marker of hyperandrogenemia in women with PCOS.

Visfatin negatively correlated with HDL cholesterol levels in women with PCOS. In contrast with the current study, a previous study reported that visfatin levels showed positive correlation with HDL cholesterol levels in lean PCOS patients [36]. This may indicate that visfatin is associated with lipid homeostasis in lean women with PCOS, but the direction of association remains unclear.

In conclusion, the current study suggests that there were no differences in serum visfatin levels between PCOS patients and the controls. However, hyperandrogenic patients showed significantly higher serum visfatin levels than those in non-hyperandrogenic patients, and visfatin levels had a positive correlation with log FAI. Further work on the probable role of visfatin in PCOS pathogenesis or hyperandrogenism is required.

Acknowledgements

This research was supported by the SNUH Research Fund funded by the Seoul National University Hospital (04-2013-0980) and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (20100009075).

Notes

^{Conflict of interest} No potential conflict of interest relevant to this article was reported.

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ORCID iDs: Young Min Choi
https://orcid.org/0000-0003-1245-0378
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