Journal List > J Korean Soc Hypertens > v.18(4) > 1089792

Bae, Kim, Choi, Ma, Lee, and Kim: Altered Regulation of Endothelin, Atrial Natriuretic Peptide, and Nitric Oxide Systems in Angiotensin II-induced Hypertension

Abstract

Background

The present study aimed to determine the changes of endothelin (ET), nitric oxide, and atrial natriuretic peptide (ANP) systems in the kidney and aorta in angiotensin (Ang) II-induced hypertension.

Methods

Male Sprague-Dawley rats were used. Ang II (100 ng·min-1·kg-1) was infused through entire time course. Fourteen days after beginning the regimen, aorta and kidney were taken. The protein expression of nitroc oxide synthase (NOS) was determined by semiquantitative immunoblotting. The mRNA expression of components of ET, NOS, ANP system was determined by real-time polymerase chain reaction.

Results

Hypertension was developed in the experimental group. mRNA expression of ET-1 in the aorta and kidney was increased. The protein expression of endothelial NOS (eNOS) was decreased in the aorta, while that of inducible NOS and neuronal NOS remained unaltered. mRNA expression of ANP, natriuretic peptide type (NPR)-A, and NPR-C was not changed in the aorta.

Conclusions

Based on these results, it seems that in Ang II-induced hypertensive rats, increased expression of ET-1 in the aorta and kidney, and decreased eNOS expression in the aorta contribute to the pathogenesis of hypertension.

Figures and Tables

Fig. 1
The mRNA expression of endothelin-1 (ET-1), endothelin receptor type A (ETAR) and type B (ETBR) in aorta (A) and kidney (B). Fluorographs show ethidium bromide stained agarose gels containing reverse transcription-polymerase chain reaction (PCR) products, and columns show real-time PCR data representing control and angiotensin (Ang) II-induced hypertensive groups. *p < 0.05 compared with control.
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Fig. 2
The mRNA expression (A) and semiquantitative immunoblotting (B) of endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS), and neuronal NOS (nNOS) in the aorta. Ang, angiotensin. *p < 0.05 compared with control.
jksh-18-146-g002
Fig. 3
The mRNA expression of atrial natriuretic peptide (ANP), natriuretic peptide receptor (NPR)-A and NPR-C in the aorta. Ang, angiotensin.
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Table 1
Changes of functional data and blood pressure
jksh-18-146-i001

*p < 0.05.

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