Abstract
Objective
The objective of this study was to evaluate the effect of intra-peritoneal nuclear factor κB (NF-κB) decoy oligodeoxynucleotide on the prevention of postoperative intra-abdominal adhesion.
Methods
Forty female BALB/c mice were randomly assigned into 2 groups. The mice were anesthetized and then midline abdominal incision was made. Peritoneal injury was given using cytobrush until deperitonealization. The experimental group (n = 20) received NF-κB decoy oligodeoxynucleotide (NF-κB decoy ODN) in the peritoneal cavity before abdominal closure, while the control group (n = 20) received saline. At 2 weeks postoperation, after the mice were euthanized, the status of intra-abdominal adhesion was evaluated. The degrees of adhesion were graded into no (grade 0), mild (grade 1), moderate (grade 2), and severe adhesion (grade 3).
Results
Two mice in the experimental group and 3 mice in the control group were dead within 1 day postoperation. In the control group, 2, 2, and 13 mice showed mild, moderate, and severe adhesion, respectively. In the experimental group, 4 mice showed no adhesion, whereas, 6 and 8 mice showed mild and moderate adhesion, respectively. The difference of degrees of adhesion between the groups was statistically significant (P <0.001).
Figures and Tables
Fig. 1
Structure of ring type-nuclear factor -κB (NF-κB) decoy oligodeoxynucleotide (From Kim et al. [23] with permission from Elsevier).
![kjog-55-244-g001](/upload/SynapseData/ArticleImage/2021kjog/kjog-55-244-g001.jpg)
Fig. 2
The representatives of the each grade of intra-abdominal adhesion severity. (A) No adhesion (grade 0). (B) Mild adhesion (grade 1). (C) Moderate adhesion (grade 2). (D) Severe adhesion (grade 3).
![kjog-55-244-g002](/upload/SynapseData/ArticleImage/2021kjog/kjog-55-244-g002.jpg)
Table 1
Adhesion score and vascularity grade description (From Yoldemir et al. [24] with permission from Elsevier)
![kjog-55-244-i001](/upload/SynapseData/ArticleImage/2021kjog/kjog-55-244-i001.jpg)
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