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Ahn, Song, Kim, Kim, Park, and Choi: Intra-peritoneal NF-κB decoy oligodeoxynucleotide decreases postoperative intra-abdominal adhesion
Original Article
Korean Journal of Obstetrics & Gynecology

Korean Journal of Obstetrics & Gynecology 2012; 55(4): 244-249.

Published online: 17 April 2012

DOI: https://doi.org/10.5468/KJOG.2012.55.4.244

Intra-peritoneal NF-κB decoy oligodeoxynucleotide decreases postoperative intra-abdominal adhesion

Byul Nim Ahn, MD1, Mi Hyun Song, MD1, Ju Hyun Kim, MD1, Kyung-Hyun Kim, PhD2, Kwan-Kyu Park, MD2, Youn Seok Choi, MD1

1Department of Obstetrics and Gynecology, Catholic University of Daegu School of Medicine, Daegu, Korea.

2Department of Pathology, Catholic University of Daegu School of Medicine, Daegu, Korea.

Corresponding author: Youn Seok Choi, MD. Department of Obstetrics and Gynecology, Catholic University of Daegu School of Medicine, 33 Duryugongwon-ro 17-gil, Nam-gu, Daegu 705-718, Korea. Tel: +82-53-650-4087, Fax: +82-53-650-4078, drcys@cu.ac.kr

Received 1 November 2011       Revised 27 December 2011       Accepted 6 February 2012

Copyright © 2012. Korean Society of Obstetrics and Gynecology

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective

The objective of this study was to evaluate the effect of intra-peritoneal nuclear factor κB (NF-κB) decoy oligodeoxynucleotide on the prevention of postoperative intra-abdominal adhesion.

Methods

Forty female BALB/c mice were randomly assigned into 2 groups. The mice were anesthetized and then midline abdominal incision was made. Peritoneal injury was given using cytobrush until deperitonealization. The experimental group (n = 20) received NF-κB decoy oligodeoxynucleotide (NF-κB decoy ODN) in the peritoneal cavity before abdominal closure, while the control group (n = 20) received saline. At 2 weeks postoperation, after the mice were euthanized, the status of intra-abdominal adhesion was evaluated. The degrees of adhesion were graded into no (grade 0), mild (grade 1), moderate (grade 2), and severe adhesion (grade 3).

Results

Two mice in the experimental group and 3 mice in the control group were dead within 1 day postoperation. In the control group, 2, 2, and 13 mice showed mild, moderate, and severe adhesion, respectively. In the experimental group, 4 mice showed no adhesion, whereas, 6 and 8 mice showed mild and moderate adhesion, respectively. The difference of degrees of adhesion between the groups was statistically significant (P <0.001).

Conclusion

Intra-peritoneal NF-κB decoy ODN reduced the severity of postoperative intra-abdominal adhesion.

Keywords: Adhesion, Inflammation, NF-kappa B, NF-kappa B decoy oligodeoxynucleotide

Figures and Tables

Fig. 1
Structure of ring type-nuclear factor -κB (NF-κB) decoy oligodeoxynucleotide (From Kim et al. [23] with permission from Elsevier).
kjog-55-244-g001
Fig. 2
The representatives of the each grade of intra-abdominal adhesion severity. (A) No adhesion (grade 0). (B) Mild adhesion (grade 1). (C) Moderate adhesion (grade 2). (D) Severe adhesion (grade 3).
kjog-55-244-g002
Table 1
Adhesion score and vascularity grade description (From Yoldemir et al. [24] with permission from Elsevier)
kjog-55-244-i001
Table 2
Grade of adhesion
kjog-55-244-i002

NF group, the group treated with intra-peritoneal NF-κB decoy oligodeoxynucleotide.

aChi-square test.

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