Abstract
Purpose
A new sclerotherapy technique by slow mechanical infusion of sclerosant was validated for treatment of microcystic lymphatic malformation (mLM).
Materials and Methods
Seventeen consecutive patients with mLM in extremities, cervicofacial area, and trunk were included (21.8 ± 21.5 years old, male:female = 5:12). All patients diagnosed as mLM were included. A total 4-32 mL 20-38% OK-432 solution was mechanically infused at the rate of 10 mL/hour into the mLM lesions. The treatment effect was estimated clinic-radiologically at the 4-month follow-up. Repeated sclerotherapy followed in the 6th month, if required. The therapeutic effect was evaluated using quantitative ultrasonographic examination including soft tissue thickness, cyst size and number.
Results
In 17 patients, total 31 infusion sclerotherapy sessions were performed and monitored for 425 ± 266 days. Fifteen patients (88%) showed improvement in all symptoms, signs, and ultrasonographic findings. In all cases, at least one finding presented improvement. The maximal number of cysts per ultrasonographic window and maximal diameter of the largest cyst decreased by 57 ± 57% and 51 ± 67%, respectively (p = 0.102, 0.004). The soft tissue thickness decreased by 18 ± 15% (p < 0.01). No significant complications such as distal lymphedema or skin necrosis occurred.
Congenital vascular anomalies including lymphatic malformation (LM) are described by different names from diverse viewpoints. LM has been described as cystic hygroma or lymphangioma based on physical and pathological features. In 1982, Mulliken and Glowacki (1) suggested a reasonable classification for congenital vascular anomalies including LM. Subsequently in 1996, the International Society for the Study of Vascular Anomalies acknowledged their nomenclature as a standard classification of congenital vascular anomalies (2). Recently, LM is further classified as microcystic, macrocystic, and mixed forms, according to the size of the abnormal lymphatic cavity. Microcystic LM is defined as a single or multiple lymphatic cysts or channels smaller than 2 cm3 in size (3).
Clinical diagnosis of microcystic LM usually depends on its features including non-compressible tissue consistency during palpation, typical dermal lesions such as vesicles or maculopapular lesions, soft tissue mass, lymph leakage, hemorrhage, and pain (4). Ultrasonography (USG) or magnetic resonance imaging (MRI) of LM aims to achieve more confirmative diagnoses and evaluate the extent and subtypes of the LM (5). In contrast to macrocystic LM showing single or multiple separate or interconnected thin-walled cysts, microcystic LM presents smaller irregular cysts with microscopic abnormal lymphatic channels. In the MRI, the microscopic abnormal lymphatic channels may appear as a solid lesion of high fluid content without an evident contrast-enhancement. In USG, the abnormal microscopic lymphatic channels usually appear as a hyperechoic infiltrative solid soft tissue lesion.
Historically, LM has been treated by surgery (5). Recently, direct puncture sclerotherapy has been used as an initial treatment of choice and achieved more favorable results than surgical treatment (3). In a prospective survey using OK-432 (Picibanil®, Chugai Pharmaceutical Co., Tokyo, Japan) as a sclerosant, the outcome of sclerotherapy was rated high in macrocystic LM and low in mixed LM. Whereas, in this prospective survey, no microcystic LM responded properly to the sclerotherapy (6). A study with repeated sclerotherapy using bleomycin A5 hydrochloride presented 78.57% effectiveness in a microcystic LM cohort. However, the response was clinically graded without quantitative analysis (7). A study with direct puncture sclerotherapy using 20 mg/mL doxycycline reported complete ablation of 179 microcysts without presenting effectiveness per patient (8). As for sclerotherapy of microcystic LM, diverse levels of its effectiveness have been reported by lesion characteristics, treatment methods, sclerosants, and result evaluation criteria (56791011121314).
Due to the multiple small isolated cysts and since microscopic dilatation of lymphatic channels may be free from infiltration of the manually injected sclerosant during sclerotherapy, the therapeutic response of microcystic LM might not be as good and the recurrence rate might be higher than in macrocystic LM. We introduced a new sclerotherapy protocol using a slow mechanical infusion technique to increase the effectiveness of sclerotherapy for microcystic LM. The new protocol was applied to microcystic LM cases containing both infiltrative channel-type and microcystic lesions. In this study, a mid-term outcome of the new sclerotherapy technique, "infusion sclerotherapy", was evaluated retrospectively.
The study protocol was approved by our Institutional Review Board (IRB). Patients' informed consents were waived by IRB due to the retrospective observational nature of the study.
All consecutive 17 patients for the year 2012 were enrolled in the subject group without exclusion. Due to an exchange of sclerosant supply, latter data were not included in the study group. The age of subjects ranged from 1 to 68 years of age with an average of 18.6 years. The male to female ratio was 5:12. The anatomical locations of the microcystic LM were head and neck (n = 7), lower extremity (n = 7), trunk (n = 1), and upper extremity (n = 2). The subjects' chief complaints comprised of dermal lesions such as vesicular maculopapular lesions (n = 6), swelling (n = 4), pain (n = 5), and leakage of lymph fluid or hemorrhage (n = 2). The demographic and clinical data of all subjects were summarized in Table 1.
Microcystic LM was diagnosed based on clinical and imaging features by an interventional radiologist, vascular surgeons, plastic surgeons, orthopedic surgeons, and dermatologists in our interdisciplinary vascular anomaly clinic by consensus of. The diagnostic criteria were based on the typical features of microcystic LM including vesicular maculopapular lesion on the skin surface, soft tissue mass with rubbery hard texture, oozing of lymphatic fluid or hemorrhagic fluid, and/or pain and tenderness.
Imaging diagnoses were performed using both MRI and Doppler ultrasonography (DUS) according to our diagnostic protocol for vascular malformation. The extent of infiltrative channel-type and/or cystic lesion was mainly evaluated in the MRI. In the DUS, a confirmative diagnosis was performed based on the imaging features such as an infiltrative lesion with small well-defined cystic component(s) smaller than 16 mm in their mean diameter without blood flow inside the cystic lesion. The diameter threshold value of 16 mm was induced to match 2 cm3 in its volume. During compression-release technique, absence of augmentation-cessation of stagnant blood flow was a clue to exclude venous malformation.
According to our diagnostic protocol for vascular malformation, if the imaging and clinical features were not confirmative, surgical biopsy was listed as the final step for a confirmative diagnosis. However, in this subject group, no pre-treatment surgical biopsy was performed.
The sclerotherapy procedures started from a pre-procedure on-site ultrasonographic evaluation of the microcystic LM. After deciding the target point for the percutaneous puncture, surgical preparation with sterilization and draping was performed. Ultrasonographic transducers were covered by dedicated sterile cover for the guidance of sclerotherapy procedure. Young patients who were potentially unable to cooperate were sedated before starting the procedure using 0.1 mg/kg midazolam (Midazolam®, Roche, Basel, Switzerland) and 1–2 mg/kg ketamine (Ketamine®, Huons, Seongnam, Korea). The sedation was maintained by repeated ketamine injection until the end of the sclerotherapy procedure. Under ultrasonographic guidance, the microcystic component was punctured using a 22–24 gauge needle. The success of the puncture could be identified by the aspiration of lymph fluid or saline influx into the cystic lesion observed under real-time ultrasonographic guidance. Small amount (1–2 mL) of iodine contrast media (Visipaque 320®, GE Healthcare AS Korea, Seoul, Korea) was injected manually into the lesion under fluoroscopic monitoring. The LM was re-confirmed by the identification of abnormally dilated lymphatic channels, communicating microcystic lesions, and/or contrast media drainage into adjacent lymphatic vessels. In cases (patient number 9 and 10 in Table 1) who revealed no cystic component in ultrasonographic images, infusion needles were inserted into the central portion of the lesions noted in both MRI and USG. In these non-cystic cases, if the spreading pattern of the contrast media presented micro channels at 1 to 3 minutes after test injection, the infusion sclerotherapy was initiated.
For infusion sclerotherapy, the punctured needle or venipuncture catheter was connected to the syringe containing diluted OK-432 solution and loaded in a mechanical infusion pump (Terufusion®, Terumo Corporation, Tokyo, Japan). The sclerosant, OK-432, was diluted using radiopaque contrast media (Lipiodol Ultra-Fluide®, Laboratoire Guerbet, Aulnay-sous-Bois, France; Visipaque 320®, GE Healthcare AS Korea) to reach 25% of its concentration. In cases using lipiodol, since lipiodol was lipophilic in contrast to hydrophilic OK-432, the solution was prepared as an emulsion form by manual mechanical mixing of 2 agents contained in interconnected syringes. The manual mixing continued for at least 1 minute. The lipiodol was chosen for LM comprising of smaller cysts or thin channels since the lipophilicity was expected to facilitate the propulsion of sclerosant solution through thin channels. When the lipiodol emulsion presented a layering border by hydrophilic-lipophilic separation, to keep the concentration of OK-432, the border zone was placed at the eccentric outlet of syringe. The loading volume of the OK-432 solution was decided subjectively based on the size of LM lesion. Initially, 8–16 mL of the OK-432 solution was loaded in to the 20-mL syringe. The sclerosant was infused at a rate of 10 mL/hour constantly in all subjects.
During the infusion, fluoroscopic surveillance was performed every 1 or 2 minutes. Intermittently, radiographic images were acquired to more clearly demonstrate the spread of the sclerosant within LM lesion, as compared to fluoroscopy. Also, ultrasonographic surveillance was performed intermittently for checking appropriate infusion of sclerosant into the cystic lesion. During the infusion of sclerosant solution, if the solution started to be drained into adjacent major normal lymphatic vessels, the infusion was stopped immediately. The normal lymphatic vessels were identified by their typical location and connection as well as tubular vascular morphology without cystic or irregularly dilated segments.
If the sclerosant coverage in the LM lesion was not sufficient due to disconnected cysts, another USG-guided puncture was performed and additional infusion sclerotherapy was continued until full coverage of the LM lesion by the infused sclerosant solution. In cases of multiple parallel needling, simultaneous infusion using multiple infusion pumps was conducted. The number of punctures needed per session ranged from 1 to 4 (average 2.0 ± 1.2). The injected volume and infusion time of the OK-432 solution ranged from 4.0 mL to 32.0 mL (average 10.0 ± 6.5 mL) and from 10 min to 120 min (average 46 ± 22 minutes), respectively. The coverage was confirmed by comparing the contrast-enhanced lesion in fluoro-radiography and lesion extent in USG or MRI.
After the completion of the sclerosant infusion, compressive dressing was applied if anatomically possible. In cases of absent normal draining lymphatic channels around the lesion, gentle massage was applied for several hours. Routinely, 5-mg Dexamethasone sodium phosphate (Dexamethasone®, Jeil Pharmaceutical, Daegu, Korea) was intravenously infused within 1 hour after sclerotherapy to prevent excessive inflammation and its complications. On the third day, the lesion area was checked to evaluate sclerotherapy-related complications such as dermal discoloration, bleb formation, lymphedema, or necrosis. If there were no complications, the patients were discharged. In 1–3 week, the patients returned to the radiology outpatient clinic to be checked for sclerotherapy-related complications a second time. If no further problems occurred, the patients were told to return in 4 months for a follow-up ultrasonographic examination. The follow-up ultrasonographic exams were performed with the same equipment, scanning technique, and radiologist as the initial exams.
The result of the infusion sclerotherapy was initially decided by follow-up USG in 4 months using the same ultrasonographic machines and scanning protocols. In the initial ultrasonographic images, recalled from picture archiving and communication system (Star PACS, Infinitt, Seoul, Korea), the largest cyst was selected as a target cyst. The longest diameter of the target cyst was measured with counting total number of cysts within the image. The soft tissue thickness was measured from dermal surface to an interface between the lesion and underlying structure such as muscular fascia or bony cortex (Fig. 1). During the follow-up USG using the same settings as the initial exam, interval changes of target cyst size, number of cysts, and soft tissue thickness were measured quantitatively. Also, the results were judged on a scale of 6 points; 0, aggravated; 1, no interval change; 2, mild improvement; 3, marked improvement; 4, near complete improvement; 5, complete improvement. When > 1 component improved, "marked improvement" was marked, whereas the "mild improvement" was marked when only 1 component improved.
The final result of the infusion sclerotherapy was decided at the vascular anomaly clinic based on an interdisciplinary consensus during the patient's second visit after 4 months. The results were evaluated as improved, persistent, or aggravated by subjective symptoms, objective signs, and image findings mainly by USG. A persistent subjective symptom was declared when the patient did not notice an evident change 4 months after sclerotherapy. A persistent sign was objectively defined when the patient's family members and doctors could not find any evidence of change in the lesion. Further treatment strategies were decided based on an interdisciplinary consensus, as well as the agreement of patients. If either improved or persistent cases were LM with accessible cysts by USG-guided puncture, additional infusion sclerotherapy was usually recommended. No further sclerotherapy was recommended in aggravated cases based on an interdisciplinary consensus, since it was regarded as an ineffective therapy. In cases of multiple sclerotherapies, the initial and the last clinical and ultrasonographic findings were compared to validate the infusion sclerotherapy.
In 17 patients, total 31 infusion sclerotherapy sessions (1 to 4 times with an average of 1.8 times per patient) were performed and monitored clinically and ultrasonographically. Total follow-up period from the initial sclerotherapy ranged from 44 to 938 days with an average of 425 ± 266 days. Patient number 8 returned to the clinic in 44 days after initial sclerotherapy with mildly improved clinical features. Fifteen patients (88%) showed improvement in all symptoms, signs, and image findings (Fig. 2). One patient (patient number 4 in Tables 1, 2) revealed no interval changes of his subjective symptom and sign despite remarkable improvement of ultrasonographic findings. Another patient (patient number 6 in Tables 1, 2) revealed aggravated lesion in USG despite of mild improvement of symptoms and signs. However, in all cases treated by infusion sclerotherapy, at least 1 component among symptom, sign, and image finding presented improvement (Tables 1, 2, Fig. 3).
During initial ultrasonographic examinations, maximal number of cysts per ultrasonographic window ranged from 0 to 7 with an average of 1 ± 1.8. Maximum diameter of the largest cyst in most crowded ultrasonographic window ranged from 0 mm to 16.0 mm with an average of 9 ± 5.7 mm. The zero count and diameter meant non-cystic LM diagnosed by fluid-rich solid soft tissue finding and pathognomic dermal vesicular lesions. The soft tissue thickness including lesion and overlying dermal structures ranged from 3.5 mm to 24.7 mm with an average of 14.5 % 6.4 mm. During the last follow-up USG, the maximal number of cysts per ultrasonographic window and maximal diameter of the largest cyst decreased by 57 ± 57% and 51 ± 67%, respectively (p = 0.102, 0.004). The soft tissue thickness decreased by 18 ± 15% (p < 0.01) (Fig. 1). The 3 ultrasonographic check points showed asynchronously, different interval changes per subject (Table 2).
Sclerotherapy-related complication issue occurred in 1 of 17 cases (5.9%). A localized mass-like microcystic LM case (patient number 4 in Tables 1, 2) showed aggravated clinical features due to persistent inflammation and intra-lesional lymphedema. After 1 month of medical treatment with antibiotics and anti-inflammatory drugs, the lymphedema and inflammation subsided. At the last follow-up USG in 483 days, cystic components disappeared within scarred mass-like lesion and the soft tissue thickness decreased up to 68%. However, due to induration and discoloration of the lesion and overlying skin, both patient's and doctors' views could not be satisfied. Based on an interdisciplinary consensus, the patient was finally treated by surgical excision. The histological examination using classic hematoxylin and eosin stain, special staining for elastic fiber, and immunohistochemistry for D2-40, Glut-1, CD31, SMA, and Prox-1 presented a LM.
No other early complication was noted in this study. Also, no delayed sclerotherapy-related complication was presented. A case of long-standing and extensive microcystic LM in the right thigh (patient number 8 in Tables 1, 2) presented mild improvement when 44-day monitoring by USG. However, in 7 months, clinical features were aggravated to the same degree as the initial lesion. Three years prior, this patient had undergone surgical dermosubcutaneous excision and subsequent skin graft. In the second year follow-up, recurrent lesion had been observed in posterior thigh adjacent to the graft margin, which induced the infusion sclerotherapy. The further aggravation in the third year occurred along the whole circumference of and within the grafted skin with conjoined lymphedema in right lower leg. The lymphedema of the right lower leg and lymphatic fluid leakage could be managed by conservative compressive dressing. In this case, the infusion sclerotherapy could achieve a transitory remission before a recurrence after 7 months. No long term image follow-up was performed for this patient.
In this study, valid results of the infusion sclerotherapy for microcystic and channel-type LM were achieved. Microcystic LM is reported to be less effective to percutaneous sclerotherapy using diverse sclerosants. A noticeable report using several sclerosants presented complete responses in 17% of 15 cases with microcystic LM. However, in the same study, the success rate was markedly lower than macrocystic LM (47%) (15). A main reason for lower success rate of sclerotherapy in microcystic LM is conceived to be non-uniform spread of sclerosant through complex lesion geometry with less connectivity. The rationale of the present study was that slow constant infusion could improve uniformity of drug delivery through the lesion by avoiding earlier rupture or occlusion of lymphatic channels.
The definition of microcystic LM was suggested as LM comprising of single or multiple cysts smaller than 2 cm3 in volume. Lesions with a cyst larger than this criterion were classified as a macrocystic LM. Combined macrocystic and microcystic LM was nominated as mixed type LM (3). In parallel, another classification guideline using the diameter of the largest cyst within LM has been suggested. The maximal diameter of cysts less than 2 cm was suggested as microcystic LM (716). In this study, microcystic LM was classified by cut-off value of 16 mm in diameter with the criterion of 2 cm3 in volume, since measuring the diameter was more practical during ultrasonographic evaluation than measuring the volume of the cysts.
In this study, the 3 ultrasonographic check points showed asynchronous interval changes. In most cases, the size of the largest cyst and soft tissue thickness decreased with statistical significance. In 9 cases (53%), despite no statistical significance, there was a trend that maximal number of cyst per ultrasonographic window presented unchanged or increased. Among these 9 cases, 7 cases presented a decreased size of the largest cyst. In addition, all the 9 cases presented improvement in clinical symptom and sign. In cases showing increased diameter of the largest cyst (n = 3), no increase in cyst number and improvement of clinical features were observed. In a single case of increased soft tissue thickness, all the other clinical and imaging features improved. Due to the complexity of image findings in the present study, systematic quantitative evaluation with majority rule was used (Table 2).
Among ultrasonographic check points, soft tissue thickness was the most consistent with clinical features. Since soft tissue swelling is a major clinical complaint, soft tissue thickness change seemed to be concordant with clinical decision. The check point cyst number did not change sensitively although size of the cysts gradually decreased (Table 2). When cysts decreased, daughter cysts developed in the adjacent area possibly due to occluded drainage routes. In this context, the importance of ultrasonographic findings is listed as soft tissue thickness, size of the largest cyst, and number of cysts, in order.
On the demographic data for our subject groups, more females than males were included in contrast to the report of no sexual predilection (17). In this study, the most prevalent lesion sites were head, neck, and lower extremity, which was concordant with a previous report (18). In the literature, the prevalent age of LM was reported as infancy and young childhood (80–90% before the age of 2 years) (18). Whereas, our entire subject cohort showed a mean age of 18.6 years and only 2 subjects were younger than the age of 2 years. Since our subject group was solely comprised of microcystic LM cases actually treated by sclerotherapy, the age distribution should be deviated from epidemiological data comprising of all subtypes of LM. Additional reasons for age discrepancy of our microcystic LM cases include less clinical implications in young subjects due to relatively shorter pathological history and a tendency to postpone active interventional or surgical treatment.
Several drugs or chemicals have been used as agents for sclerotherapy of LM, including bleomycin, doxycycline, ethanol, and OK-432 (3192021). Despite the complications by penetration into adjacent tissue, ethanol has been used as the strongest sclerosant for more definite results (102223). Bleomycin was introduced for LM sclerotherapy in very early trials (24). However, it may cause fatal complication such as pulmonary interstitial fibrosis or hypersensitivity (2526). Among the cases in this study, no fatal complications developed after infusion sclerotherapy using OK-432. Yang et al. (11) recently reported the application of bleomycin A5 in subjects including balanced numbers of LM subtypes in head and neck with 100% response rate. In this study, since the effect of sclerotherapy was evaluated by means of size change of the target cysts within LM, the response might be overestimated, as compared to its clinical relevance. Recently, a successful repeated sclerotherapy for microcystic LM using bleomycin was reported without relevant complication (27). Shergill et al. (28) reported an excellent response of microcystic LM to doxycycline sclerotherapy although the cohort was small. No fatal complications by doxycycline sclerotherapy have been published, to our best knowledge. However, the overall complication rate of doxycycline was reported to be significantly associated with microcystic subtype and greater dose of doxycycline (29).
In this study, the infusion sclerotherapy technique was evaluated originally using OK-432, which was a routine sclerosant in our institute. OK-432 is the attenuated low-virulent strain of group A Streptococcus pyognes of human origin. By its immunopharmacologic inflammatory reaction, endothelial lining structures of LM are destroyed and adhered representing the sclerosing effect (30). Whereas OK-432 is not approved for use in American and European countries, Asian countries where our institute locates approved its use as a sclerosant. The effect of OK-432 sclerotherapy of microcystic LM was summarized in a meta-analysis comprising 48 cases (13). In the overall results, 60% of cases showed good responses. In this study, 88% of the microcystic LM cases treated by the infusion sclerotherapy using OK-432 showed improvement as agreed on by all patients and doctors, and confirmed by images.
OK-432 sclerotherapy for LM could be regarded as a safe technique. However, in case of infusion sclerotherapy, higher volume of sclerosant should be infused into both microcystic and microchannel components of microcystic LM. Due to the slower infusion rate and weaker infusion power compared to manual injection, the sclerosant could travel further through microchannels with decreased possibility of extravasation into surrounding soft tissue. By thoroughly filling the abnormal LM spaces, subsequently, the sclerosant could have a higher chance to be drained into adjacent normal lymphatic channels. Based on these facts, higher prevalence of complications in adjacent lymphatic tissue such as lymphedema are possible. Therefore, the application of infusion sclerotherapy technique on critical lymphatic bridgehead such as inguinal or axillary area should be done with caution and may require further evaluation for its safety.
A major limitation of this study was that the decision of symptoms and signs was performed subjectively by patients and surroundings, including the doctors. However, since the improvement was based on consensus of a multi-disciplinary group, objectivity might be preserved. The second limitation was the ultrasonographic improvement of microcystic LM was decided by radiologists who were not blinded to sclerotherapy information. However, introduction of a systematic quantitative evaluation of image findings attempted to minimize this limitation. The third limitation was that the OK-432 is not available for world-wide use. Although availability of the OK-432 is limited to some countries where out institute locates, its effectiveness as a sclerosant is accepted in the literature (13). Furthermore, in this study, the drug delivery technique was a main focus rather than any specific property of OK-432. The fourth limitation was that the sclerosant was an emulsion of lipophilic lipiodol and hydrophilic OK-432 that was infused for a long time. During delayed infusion, both components should be separated with layering. To maintain the concentration of OK-432 during delayed infusion, in this study, the layer between OK-432 and lipiodol was adjusted to the syringe outlet orifice. In addition, due to well-acknowledged embolic effect of lipiodol, evaluation of pure benefit of infusion sclerotherapy may be limited as compared to reported data with hydrophilic contrast media (162829). The fifth limitation was that all the follow-up imaging was performed using USG alone. Not every patient received MR exam for their routine follow-up imaging due to financial burden in our country. The objectiveness of ultrasonographic decision may have limitation for accurate quantitative measurement of interval change. In this study, ultrasonographic evaluation was based on qualitative and semi-quantitative ways with a systematic stratification to minimize subjective bias by observer. The last limitation was that the infusion sclerotherapy technique was applied only to the OK-432 cohort due to the size of cohort in our institute. The feasibility evaluation for the infusion sclerotherapy should be continued in cohorts with the other sclerosants.
In conclusions, a new sclerotherapy technique for microcystic LM i.e., infusion sclerotherapy was suggested and verified. Infusion sclerotherapy was a safe and effective treatment technique for microcystic LM, with favorable outcome. For more extensive and safe application of the infusion sclerotherapy, more delicate guideline should be developed through more extensive application. In addition, a prospective randomized trials comparing the infusion sclerotherapy and conservative technique is expected.
Figures and Tables
Fig. 1
A 2-year-old girl (patient number 13 in Tables 1, 2) who presented with soft tissue swelling at left knee area was diagnosed as microcystic lymphatic malformation.
A. Initial ultrasonography demonstrates infiltrative lesion containing multiple small cysts in subcutaneous layer of medial aspect of left knee joint. Soft tissue thickness is 16.2 mm.
B. Post-sclerotherapy 20-month follow-up ultrasonography shows markedly improved lesion with near disappearance of cysts. Soft tissue thickness was reduced to 11.3 mm.
FC = femoral condyle, MCL = medial collateral ligament, TC = tibial condyle
![jksr-74-26-g001](/upload/SynapseData/ArticleImage/2016jksr/jksr-74-26-g001.jpg)
Fig. 2
A 17-year-old man (patient number 15 in Tables 1, 2) presented a soft tissue mass with vague pain and tenderness in the left temporal area. Also, the patient complained of typical tiny dermal vesicles with intermittent fluid discharge. A poorly-defined non-compressible soft tissue mass was palpated. Clinical impression was agreed as lymphatic malformation.
A. The initial ultrasonography reveals an infiltrative soft tissue lesion with multiple non-uniform small cystic and channel-like components (arrows) in subcutaneous fat layer of the left temporal area.
B. At the early stage of infusion sclerotherapy, microcystic components are filled by sclerosant containing OK-432 and lipophilic contrast media. The infusion was done by an electromechanical infusion pump at the speed of 10 mL/hr.
C. In 2 minutes after infusion start, the sclerosant infiltrates into adjacent microcysts and channels (arrows).
D. The second lesion area was punctured and sclerosant was infused in parallel. In 21 minutes after initial infusion start, the lower lesion is fully filled by sclerosant with spill to adjacent normal-looking lymphatic channels (arrowheads). The newer upper lesion shows numerous microcysts interconnected by small channels (arrows).
E. In 29 minutes, more microcysts in further distance are filled by sclerosant (arrow).
F. In 50 minutes of infusion, no newly filled microcyst is noted and adjacent normal-looking small lymphatic channels are opacified (arrow). In lower lesion, opacification of normal lymphatic vessel is not progressed due to infusion stop (arrowhead).
G. Ultrasonography was performed in 1 year after the infusion sclerotherapy. In same scope and plane as the initial image (A), cystic and channel lesions (arrows) show decreased sizes. Also, clinical symptom and sign are improved markedly.
![jksr-74-26-g002](/upload/SynapseData/ArticleImage/2016jksr/jksr-74-26-g002.jpg)
Fig. 3
Infusion sclerotherapy presented an improvement of symptoms, signs, and image findings in 16, 16, and 16 cases among 17 patients, respectively.
![jksr-74-26-g003](/upload/SynapseData/ArticleImage/2016jksr/jksr-74-26-g003.jpg)
Table 1
Microcystic Lymphatic Malformation Cases Treated with Infusion Sclerotherapy and Their Clinical Follow-Up Results
![jksr-74-26-i001](/upload/SynapseData/ArticleImage/2016jksr/jksr-74-26-i001.jpg)
Table 2
Systematic Evaluation of Ultrasonographic Findings During Initial and the Last Follow-Up Examinations
![jksr-74-26-i002](/upload/SynapseData/ArticleImage/2016jksr/jksr-74-26-i002.jpg)
Acknowledgments
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT&Future Planning (NRF-2015R1A2A2A03005433).
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