Journal List > J Korean Soc Radiol > v.68(4) > 1087238

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Chung, Jeon, and Lee: Comparative Study of Compensatory Liver Regeneration in a Rat Model: Portal Vein Ligation Only versus Sequential Ligation of the Portal Vein and Hepatic Artery
Interventional Radiology
Journal of the Korean Society of Radiology

Journal of the Korean Society of Radiology 2013; 68(4): 289-296.

Published online: 10 April 2013

DOI: https://doi.org/10.3348/jksr.2013.68.4.289

Comparative Study of Compensatory Liver Regeneration in a Rat Model: Portal Vein Ligation Only versus Sequential Ligation of the Portal Vein and Hepatic Artery

Soo Young Chung, MD1, Gyeong Sik Jeon, MD2, Byungmo Lee, MD3

1Department of Pathology, Dongnam Institute of Radiological & Medical Sciences, Busan, Korea.

2Department of Radiology, CHA Bundang Medical Center, College of Medicine, CHA University, Seongnam, Korea.

3Department of Surgery, Seoul Paik Hospital, Inje University College of Medicine, Seoul, Korea.

Corresponding author: Gyeong Sik Jeon, MD. Department of Radiology, CHA Bundang Medical Center, College of Medicine, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, Korea. Tel. 82-31-780-5382, Fax. 82-31-780-5381, hae0820@naver.com

Received 9 November 2012       Accepted 8 January 2013

Copyright © 2013 The Korean Society of Radiology

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To compare the volume change and the regenerative capacity between portal vein ligation (embolization) (PVL) and heterochronous PVL with hepatic artery ligation (HAL) in a rodent model.

Materials and Methods

The animals were separated into three groups: group I, ligation of the left lateral and median portal vein branches; group II, completion of PVL, followed by ligation of the same branches of the hepatic artery after 48 h; control group, laparotomy without ligation was performed. Five rats from each group were sacrificed on 1, 3, 5, and 7 days after the operation. Volume change measurement, liver function tests and immunohistochemical analysis were performed.

Results

The volume of the nonligated lobe between groups I and II was not significantly different by day 5 and day 7. Mean alanine aminotransferase and total bilirubin levels were significantly higher in group II, while the albumin level was higher in group I. Both c-kit- and MIB-5-positive cells used in the activity detection of regeneration were more prevalent in group I on day 1, 3, and 5, with statistical significance. There was no operation related mortality.

Conclusion

PVL alone is safe and effective in compensatory liver regeneration. Performing both PVL and HAL does not confer any additional benefits.

Keywords: Portal Vein, Hepatic Artery, Ligation, Liver Regeneration

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