Journal List > J Korean Soc Radiol > v.62(5) > 1086766

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Kwon, Kang, and Hong: Micro-CT Arthrographic Analysis of Monosodium Iodoacetate-Induced Osteoarthritis in Rat Knees
Musculoskeletal Radiology
Original Article

Journal of the Korean Society of Radiology 2010; 62(5): 483-490.

Published online: 31 May 2010

DOI: https://doi.org/10.3348/jksr.2010.62.5.483

Micro-CT Arthrographic Analysis of Monosodium Iodoacetate-Induced Osteoarthritis in Rat Knees

Jong Won Kwon, M.D., Heung Sik Kang, M.D.2, 3, Sung Hwan Hong, M.D.3

1Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, Korea.

2Department of Radiology, Seoul National University Bundang Hospital, Korea.

3Department of Radiology and Institute of Radiation Medicine, Seoul National University College of Medicine, Korea.

Address reprint requests to: Heung Sik Kang, M.D., Department of Radiology, Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam-si, Gyeongi-do, 463-707, Korea. Tel. 82-31-787-7619, Fax. 82-31-787-4011, kanghs@radcom.snu.ac.kr

Received 21 December 2009       Accepted 26 January 2010

Copyright © 2010 The Korean Society of Radiology

Abstract

Purpose

To evaluate the arthrographic findings of MIA-induced osteoarthritis in rat knees using the micro-CT arthrography.

Materials and Methods

Intra-articular monosodium iodoacetate (MIA) injection-induced arthritis was induced in the right knees of twelve rats; their left knees served as the control group. Eight weeks after MIA injection, micro-CT arthrography was performed on each knee. We measured the thickness of retro-patellar cartilages, the distances of tibio-femoral joint space, subchondral bone plate thickness, tibial epiphyseal height, and transverse patellar diameter. Subchondral trabecular bone indices were measured in the tibial lateral condylar epiphysis. The data were analyzed statistically using a paired t-test.

Results

The retro-patellar articular cartilage showed thinning on the right side that had been induced to develop osteoarthritis. The right knees showed a significant reduction in the distance of the tibio-femoral joint space, prominent patellar osteophytes, and the resorption of subchondral bone. Among the subchondral trabecular bone indices, percent bone volume, and trabecular thickness was reduced on the right side.

Conclusion

The articular cartilage thickness of MIA-induced arthritis model could be measured using micro-CT arthrography. It was possible to evaluate the osteoarthritic findings including the change in subchondral bone plate thickness, osteophyte formation, and subchondral bone resorption, as well as quantitatively analyze the trabecular bone indices.

Keywords: X-Ray Microtomography, Arthrography, Osteoarthritides, Cartilage, Articular

Figures and Tables

Fig. 1

Measurement of retropatellar cartilage thickness, joint space of tibio-femoral joint and height of tibial epiphysis.

A. Micro-CT arthrographic image shows three locations for measurement of retropatellar cartilage thickness in axial plane that are labeled 'Medial', 'Center', and 'Lateral'.
B. Micro-CT arthrographic image shows three locations for measurement of retropatellar cartilage thickness in sagittal plane that are labeled 'Upper', 'Middle', and 'Lower'.
C. Coronal reconstruction of micro-CT arthrographic images shows location of measurement of joint space of medial tibiofemoral joint (arrowheads) and height of lateral tibial epiphysis (arrow).
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Fig. 2

A. A cylindrical VOI is defined at lateral tibial epiphysis at CT Analyser program window.

B. 3D trabecular indices are measured with CT Analyser program.
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Fig. 3

Micro-CT arthrographic finding of MIA-induced osteoarthritis in rat

A. Micro-CT arthrographic image shows irregular thinning of retropatellar cartilage (black arrow) and prominent osteophyte of patella (white arrow).
B. Sagittal reconstruction of micro-CT arthrographic image shows resorption of subchondral bone plates of patello-femoral joint (arrows).
C. Coronal reconstruction of micro-CT arthrographic image shows bony resorption of intercondylar notch of tibia and lateral margin of medial femoral condyle. There is no definitive marginal osteophyte in tibio-femoral joint (arrows).
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Table 1

Retropatellar Cartilage Thickness (µm) on Mid-axial Images

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Rt.: right side, osteoarthritis group; Lt.: left side, control group; SD : standard deviation

Table 2

Retropatellar Cartilage Thickness (µm) on Mid-sagittal Images

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Rt.: right side, osteoarthritis group; Lt.: left side, control group; SD : standard deviation

Table 3

Thickness (µm) of Subchondral Bone Plate, Epiphysis Height, Joint Space and Patellar Transverse Diameter

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MTP: medial tibial plateau; LTP: lateral tibial plateau; Rt.: right side, osteoarthritis group; Lt.: left side, control group

Table 4

Summary of Trabecular Indices

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*: p-value < 0.05; Rt.: right side, osteoarthritis group; Lt.: left side, control group

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