Journal List > J Korean Dent Soc Anesthesiol > v.14(2) > 1084362

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Yoon, Shin, Park, Kim, Woo, Yoon, and Kim: Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast
Original Article

Journal of the Korean Dental Society of Anesthesiology 2014; 14(2): 107-114.

Published online: 27 May 2016

DOI: https://doi.org/10.17245/jkdsa.2014.14.2.107

Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast

Ji Uk Yoon, Sang Wook Shin, Bong Soo Park*, Yong Ho Kim*, Mi Na Woo†, Ji Young Yoon‡, Cheul Hong Kim‡

Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University, Gyeongnam, Korea.

*Department of Oral Anatomy, School of Dentistry, Pusan National University, Gyeongnam, Korea.

†Department of Dental Anesthesia and Pain Medicine, Pusan National University Dental Hospital, Gyeongnam, Korea.

‡Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Gyeongnam, Korea.

Corresponding Author: Sang Wook Shin, Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Beomeo-ri, Mulgeumeup, Yangsan-si, Gyeongsangnam-do 626-787, Korea. Tel: +82.55.360.5370, Fax: +82.55.360.5369, shinsw@pusan.ac.kr

Received 24 June 2014       Revised 3 July 2014       Accepted 4 July 2014

Copyright © 2014 Journal of the Korean Dental Society of Anesthesiology

(open-access, http://creativecommons.org/licenses/by-nc/3.0/):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Angiogenesis has been recognized an essential precondition for osteogenesis. Because reduction and disruption of the blood supply to tissue cause tissue hypoxia, pathological bone loss affected by hypoxia often can occur in various clinical conditions. The effects of propofol on the process of osteogenesis have received little direct attention. Therefore, we investigated the effect of propofol on the growth and function of osteoblasts under hypoxic condition.

Methods

After propofol (3, 30, 300 µM) preconditioning for 2 hours, hFOB 1.19 human osteoblast cells were cultured under 1 % oxygen tension for 48 hours. Using real time PCR and western blot analysis, we analyzed the expression of, BMP-2, TGF-β1, type I collagen, osteocalcin, HIF-1s and Akt. Cell viability was also determined by MTT assay.

Results

Propofol preconditioning on hypoxic-cultured osteoblast promoted the expressions of BMP-2, TGF-β1, type I collagen and osteocalcin and induced hypoxia-mediated HIF-1 activation and the expression of Akt protein. Propofol with 300 µM significant decreased cell viability compared to control.

Conclusions

Clinically relevant concentrations of propofol are not cytotoxic to hypoxic osteoblasts in vitro. Propofol preconditioning on hypoxic-cultured osteoblast stimulates proliferation and differentiation of osteoblast through induced expression of BMP-2, TGF-β1, type I collagen and osteocalcin. Propofol might promote angiogenesis and bone regeneration under hypoxic condition.

Keywords: propofol, hypoxi, osteoblast

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