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Jung and Woo: T Cell Receptor Rearrangement in NKT Cells Differentiated from Cord Blood CD34+ Cells
Original Article

The Ewha Medical Journal 2003; 26(2): 137-142.

Published online: 30 June 2003

DOI: https://doi.org/10.12771/emj.2003.26.2.137

T Cell Receptor Rearrangement in NKT Cells Differentiated from Cord Blood CD34+ Cells

Yun-Jae Jung, So-Youn Woo

Department of Microbiology, College of Medicine, Ewha Womans University, Korea.

Copyright © 2003. Ewha Womans University School of Medicine

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives

To investigate the time of rearrangement of the TCR gene in the process of NKT cell differentiation from CD34+ human cord blood cells in vitro.

Methods

We isolated the CD34+ human cord blood cells and induced the differentiation of NKT cells by liquid culture including IL-15, FL and SCF for 30 days. In order to detect the time of TCR gene rearrangement in differentiated NKT cell, we performed PCR for TCR-rearrangement excision circles (TRECs) with isolated DNA.

Results

Signal joint TREC first appeared on day 4 or day 8 and continuously existed until day 30. Between day 9 and day 21, cells showed high output of coding joint TRECs after signal joint rearrangement.

Conclusion

In differentiated NKT cells, TCR gene rearrangement started within a week after culture started and mostly occurred in 2 to 3 weeks after culture started.

Keywords: NKT cell, CK34+ cells, TCR gene rearrangement

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