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Byun and Kim: Establishment of Three Dimensional In Vitro Laboratory Model in Squamous Cell Carcinoma of the Head and Neck: Spheroid Model and Raft Culture Model
Original Article

The Ewha Medical Journal 2001; 24(1): 15-21.

Published online: 31 March 2001

DOI: https://doi.org/10.12771/emj.2001.24.1.15

Establishment of Three Dimensional In Vitro Laboratory Model in Squamous Cell Carcinoma of the Head and Neck: Spheroid Model and Raft Culture Model

Sung-Wan Byun, Chun Dong Kim

Department of Otolaryngology, College of Medicine, Ewha Womans University, Korea.

Copyright © 2001. Ewha Womans University School of Medicine

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective

To establish new in vitro model systems that better reflect in vivo condition, multicellular tumor spheroids(MTS) and raft culture were developed using cell lines of squamous cell carcinoma(SCCHN) of the head and neck in these 3-dimensional systems.

Materials and Methods

Four SCCHN cell lines were used for MTS and raft culture.

Results

All cell lines formed MTS, but only Tu-138 showed a good stratification at the airliquid interface in the raft culture system.

Conclusions

MTS and raft culture system were established successfully from the SCCHN cell lines.

Keywords: Multicellular tumor spheroid, Raft culture, Squamous cell carcinoma of the head and neck

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