Journal List > J Nutr Health > v.49(6) > 1081464

J Nutr Health. 2016 Dec;49(6):401-410. Korean.
Published online December 31, 2016.  https://doi.org/10.4163/jnh.2016.49.6.401
© 2016 The Korean Nutrition Society
Four months of magnetized water supplementation improves glycemic control, antioxidant status, and cellualr DNA damage in db/db mice
Hye-Jin Lee and Myung-Hee Kang
Department of Food & Nutrition, Daedeok Valley Campus, Hannam University, 1646 Yuseongdaero, Yuseong-ku, Daejeon 34124, Korea.

To whom correspondence should be addressed. tel: +82-42-629-8791, Email: mhkang@hnu.kr
Received November 17, 2016; Revised December 11, 2016; Accepted December 15, 2016.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

Water is magnetically charged upon contact with a magnet. Although magnetic water products have been promoted since the 1930's, they have not received wide acceptance since their effectiveness is still in question; however, some have reported their therapeutic effects on the body, especially the digestive, nervous, and urinary systems.

Methods

In this study, the effect of magnetized water on glycemic control of 14 diabetic mice (CB57BK/KsJ-db/db) in comparison with 10 control mice (CB57BK/KsJ-db/+(db/+)) was investigated. Seven diabetic control (DMC) mice and seven diabetic mice + magnetized water (DM+MW) were kept for 16 weeks, followed by intraperitoneal glucose tolerance test (IPGTT). Weekly blood glucose was measured from tail veins. Blood obtained from heart puncture was used for HbA1c analysis.

Results

Blood glucose level showed a significant difference starting from the 10th week of study (496.1 ± 10.2 mg/dl in DMC vs. 437.9 ± 76.9 mg/dl in DM+MW). Blood glucose followed by IPGTT showed no significant difference between groups at 0, 30, 60, 90, and 120 min, although glucose level at 180 min was significantly reduced in DM+MW mice. Plasma insulin level in DM+MW groups was only 39.5% of that of DMC groups (5.97 ± 1.69 ng/ml in DMC vs. 2.36 ± 0.94 ng/ml in DM+MW). Levels of HbA1c were 12.4% and 9.7% in DMC and DM+MW groups, respectively.

Conclusion

These results show the promising therapeutic effect of magnetized water in regulating blood glucose homeostasis; however, long-term supplementation or mechanistic study is necessary.

Keywords: diabetic mice; magnetized water; glycemic control; HbA1c; insulin

Figures


Fig. 1
Effect of magnetized water on blood glucose in db/db mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, dia betes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Values within a group with different alphabet are significantly different at p < 0.05 by Duncan's multiple range test.
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Fig. 2
Effect of magnetized water on area under the blood glucose curve after oral glucose tolerance test in db/db mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7); AUC, area under the curve. Values with different alphabet are significantly different at p < 0.05 by Duncan's multiple range test.
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Fig. 3
Effect of magnetized water on plasma insulin in db/db mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Bar with different letters are significantly different at p < 0.05 after Duncan’s multiple range test.
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Fig. 4
Effect of magnetized water on blood HbA1c in db/db mice Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Bar with different letters are significantly different at p < 0.05 after Duncan’s multiple range test.
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Fig. 5
Protective effect of magnetized water on blood DNA damages in db/db mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Bar with different letters are significantly different at p < 0.05 after Duncan’s multiple range test.
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Fig. 6
Protective effect of magnetized water on liver DNA damages in db/db mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Bar with different letters are significantly different at p < 0.05 after Duncan’s multiple range test.
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Tables


Table 1
Composition of experimental diet1)
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Table 2
Body weight gain, food intake and water intake of mice
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Table 3
Effect of magnetized water on plasma TRAP and erythrocyte antioxidant enzymes in db/db mice
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