Journal List > J Nutr Health > v.48(2) > 1081382

J Nutr Health. 2015 Apr;48(2):157-166. Korean.
Published online April 30, 2015.  https://doi.org/10.4163/jnh.2015.48.2.157
© 2015 The Korean Nutrition Society
Effect of water extract and distillate from the mixture of black goat meat and medicinal herb on osteoblast proliferation and osteoclast formation
Hyo-Nam Song,1 Kang-Hyun Leem,2 and In-Sook Kwun3
1School of Integrated Oriental Medical Bioscience, Semyung University, Chungbuk 390-711, Korea.
2College of Korean Medicine, Semyung University, Chungbuk 390-711, Korea.
3Department of Food Science and Nutrition, Andong National University, Gyeongbuk 760-749, Korea.

To whom correspondence should be addressed. tel: +82-43-649-1430, Email: hnsong@semyung.ac.kr
Received January 29, 2015; Revised February 13, 2015; Accepted March 17, 2015.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

The effects of water extract and distillate from the mixture of black goat meat and medicinal herb on MG-63 osteoblast proliferation and mouse bone marrow derived osteoclast formation were investigated.

Methods

Proximate composition, volatile basic nitrogen (VBN), mineral content, free amino acid composition and free fatty acid composition in black goat meat were determined. Water extract and distillate were prepared with three groups; goat meat only (BG-E, BG-D), six herbs added group (BG-E6, BG-D6), and eight herbs added group (BG-E8, BG-D8). Osteoblast proliferation, mineralization and calcium uptake activity of MG-63 cells were measured and tartrate resistant acid phosphatase activity of osteoclasts was analyzed.

Results

Black goat meat had remarkably low fat and high level of calcium. Glutamic acid was the most abundant amino acid. Herbs added extract groups (BG-E6 and BG-E8) showed increased MG-63 cell proliferation in a concentration dependent manner, while all the distillates did not show the effect. All extracts and distillates showed significantly increased osteoblast mineralization depending on the concentration. In particular, herb added extract, BG-E6, increased 170.3% of control and the distillate of BG-D and BG-D6 increased up to 168.5% and 159.8%, respectively. Calcium uptake activities of all water extracts showed remarkable increase of BG-E6 and BG-E8 up to 615.5% and 628.1% of control, respectively. Ditillates had no effect except BG-D6. All water extracts significantly reduced the activity of tartrate-resistant acid phosphatase (TRAP) in osteoclasts derived from mouse bone marrow.

Conclusion

Combination of black goat meat and medicinal herb increased the MG-63 cell proliferation and effectively inhibited osteoclast differentiation in both water extracts and distillate of them, which implies that they could be used as potent functional food materials for bone health.

Keywords: black goat meat; medicinal herb; MG-63 cell; osteoblast; osteoclast

Figures


Fig. 1
Scheme of manufacturing black goat extract and its distilled extract
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Fig. 2
Effect of black goat meat and medicinal herb mixture on proliferation of human osteoblast MG-63 cells. D10x, 1/10 dilution; D80x, 1/80 dilution; D640x, 1/640 dilution. Data shown are the mean and SD values of three independent samples. The treatments sharing a common alphabet are not statistically significant at 5% level.
Click for larger image


Fig. 3
Effect of black goat meat and medicinal herb mixture on mineralization of human osteoblast MG-63 cells. D10x, 1/10 dilution; D80x, 1/80 dilution. Data shown are the mean and SD values of three independent samples. The treatments sharing a common alphabet are not statistically significant at 5% level.
Click for larger image


Fig. 4
Effect of black goat meat and medicinal herb mixture on calcium uptake of human osteoblast MG-63 cells. D10x, 1/10 dilution; D80x, 1/80 dilution; D640x, 1/640 dilution. Data shown are the mean and SD values of three independent samples. The treatments sharing a common alphabet are not statistically significant at 5% level.
Click for larger image


Fig. 5
Effect of black goat meat and medicinal herb mixture on TRAP activity. Data shown are the mean and SD values of three independent samples. The treatments sharing a common alphabet are not statistically significant at 5% level.
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Tables


Table 1
Proximate composition and volatile basic nitrogen (VBN) of black goat meat
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Table 2
Mineral contents of black goat meat (Unit: mg%)
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Table 3
Amino acid composition of fore limb meat of black goat (Unit: mg/100 g)
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Table 4
Fatty acid composition of fore limb meat of black goat (Unit : %)
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Notes

This work was supported by the research grant from Semyung University in 2013.

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