Journal List > J Nutr Health > v.48(2) > 1081381

J Nutr Health. 2015 Apr;48(2):149-156. Korean.
Published online April 30, 2015.  https://doi.org/10.4163/jnh.2015.48.2.149
© 2015 The Korean Nutrition Society
Effects of combined intervention of isoflavone supplementation and exercise on bone metabolism in growing rats
Yun-Jung Jung and Mi-Ja Choi
Department of Food and Nutrition, Keimyung University, Daegu 704-701, Korea.

To whom correspondence should be addressed. tel: +82-53-580-5874, Email: choimj@kmu.ac.kr
Received March 12, 2015; Revised April 06, 2015; Accepted April 09, 2015.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

This study examined the effects of combined intervention of isoflavones and exercise on bone mineral density, bone mineral content, and biochemical bone markers.

Methods

Forty rats were divided into four groups; Control, Isoflavones (IF), Isoflavones + Running (IFR), and Isoflavones + Swimming (IFS). All of the rats in this study were fed an experimental diet and deionized water ad libitum for nine weeks. Bone mineral density (BMD) and bone mineral content (BMC) were estimated using PIXImus (GE Lunar Co, Wisconsin.) in spine and femur nine weeks after feeding or training.

Results

The combined intervention did not affect weight gain, mean food intake, or food efficiency ratio. The serum concentrations of ALP and osteocalcin were not significantly different by combined intervention. The urinary DPD crosslinks values were not significantly different by combined intervention. There were no significant differences in serum PTH, calcitonin, and estradiol among all groups. Spine BMD, spine BMC and femur BMC were not significantly different by combined intervention. However, femur BMD was significantly higher in the IFR group than in the control group. Compared with the control group, spine BMD, spine BMC, and femur BMD per weight were markedly increased in the isoflavones supplementation and combined intervention group. In addition, femur BMC per weight was significantly higher in the IFS group than in the control group. Compared with the isoflavones supplemented group, BMD and BMC were not significantly different by combined intervention.

Conclusion

It can be concluded that isoflavones supplementation or combined intervention of isoflavone and exercise had a beneficial effect on spine and femur peak bone mass in growing female rats.

Keywords: isoflavones; exercise; combined intervention; bone mineral density; bone mineral content

Tables


Table 1
Composition of experimental diets (g/kg of diet)
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Table 2
Effects of isoflavones and exercise on weight gains, mean food intake and food intake efficiency ratio (FER) in growing female rats
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Table 3
Effects of isoflavones and exercise on serum alkaline phosphatase (ALP) and osteocalcin in growing female rats
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Table 4
Effects of isoflavones and exercise on deoxypyridinoline (DPD), creatinine and crosslinks value in growing female rats
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Table 5
Effects of isoflavones and exercise on serum parathyroid hormone (PTH), calcitonin and estradiol in growing female rats
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Table 6
Effects of isoflavones and exercise on spine bone mineral density (BMD) and bone mineral content (BMC)
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Table 7
Effects of isoflavones and exercise on femur bone mineral density (BMD) and bone mineral content (BMC)
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