Journal List > J Nutr Health > v.48(2) > 1081379

J Nutr Health. 2015 Apr;48(2):133-139. Korean.
Published online April 30, 2015.
© 2015 The Korean Nutrition Society
Effects of a Pueraria lobata-root based combination supplement containing Rehmannia glutinosa and aerobic exercise on improvement of metabolic dysfunctions in ovariectomized rats
Sang A Oh,1 Hyang Mok Ok,1 Hye Jin Kim,2 Won Jun Lee,2 and Oran Kwon1
1Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea.
2Department of Exercise Science, College of Health Sciences, Ewha Womans University, Seoul 120-750, Korea.

To whom correspondence should be addressed. tel: +28-2-3277-6860, Email:
Received December 03, 2014; Revised February 03, 2015; Accepted March 27, 2015.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.



There is a fair amount of evidence indicating that increased risk of obesity and insulin resistance is associated with postmenopausal state, but can be modulated by diet and exercise. In this study, we explored whether a Pueraria lobata root-based supplement containing Rehmannia glutinosa (PR) and/or aerobic treadmill exercise can modify the metabolic changes associated with estrogen deficiency.


Seventy rats were randomly assigned to the following groups for 8 weeks (n=10 per group): SHAM, sham-operated; PR0, ovariectomized (OVX) control; PR200, OVX with PR200 mg/kg B.W; PR400, OVX with PR400 mg/kg B.W; EPR0, OVX with exercise; EPR200, OVX with exercise and PR200 mg/kg B.W; EPR400, OVX with exercise and PR400 mg/kg B.W.


OVX induced significant increases in body weight, food intake, fat mass, LDL-cholesterol, and fasting blood glucose, confirming induction of menopausal symptoms. PR supplementation or exercise significantly suppressed the above mentioned changes through different regulatory elements in adipose tissue: PR supplement upregulated adiponectin gene expression and aerobic exercise upregulated adiponectin and insulin receptor gene expression and a combination of PR supplement and aerobic exercise showed an additive effect on adiponectin gene expression.


Taken together, the results of this study suggest that PR supplement has a potential to provide health benefits in OVX rats through leptin and adiponectin secretion. In addition, the data suggest that combination of exercise and PR would have additive effects on metabolic dysfunction associated with estrogen deficiency.

Keywords: Pueraria lobata; Rehmannia glutinosa; aerobic exercise; metabolic dysfunction; ovariectomized rats


Fig. 1
Effects of PR and aerobic exercise on leptin and adiponectin gene expression the retroperitoneal fat of ovariectomized rats: (A) leptin and (B) adiponectin. SHAM, sham operated; PR0, ovariectomized control; PR200, ovariectomized with PR200 mg/kg B.W; PR400, ovariectomized with PR400 mg/kg B.W; EPR0, ovariectomized with exercise; EPR200, ovariectomized with exercise and PR200 mg/kg B.W; EPR400, ovariectomized with exercise and PR400 mg/kg B.W. *p < 0.05 SHAM vs. PR0. Values not sharing the same letter were significantly different in the OVX group at p < 0.05.
Click for larger image

Fig. 2
Effects of PR and aerobic exercise on IR gene expression in the organ of ovariectomized rats: (A) liver and (B) adipose tissue. SHAM, sham operated; PR0, ovariectomized control; PR200, ovariectomized with PR200 mg/kg B.W; PR400, ovariectomized with PR400 mg/kg B.W; EPR0, ovariectomized with exercise; EPR200, ovariectomized with exercise and PR200 mg/kg B.W; EPR400, ovariectomized with exercise and PR400 mg/kg B.W; IR, insulin receptor. *p < 0.05 SHAM vs. PR0. Values not sharing the same letter were significantly different in the OVX group at p < 0.05.
Click for larger image


Table 1
Effects of PR and aerobic exercise on final body weight, food intake, and fat mass in ovariectomized
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Table 2
Effects of PR and aerobic exercise on lipid and glucose metabolism in ovariectomized rats
Click for larger image


This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project title: Research on New Functional Food Materials with Domestic Agricultural Products, Project No. PJ00845002)" Rural Development Administration, Republic of Korea.

1. Couse JF, Korach KS. Estrogen receptor null mice: what have we learned and where will they lead us? Endocr Rev 1999;20(3):358–417.
2. Yeum KH, Lee HJ, Yeo SM, Yoo JH, Kim RH. Relation of regular exercise habit to bone mineral density in Korean climacteric women. J Korean Geriatr Soc 2006;10(1):23–30.
3. Manolio TA, Furberg CD, Shemanski L, Psaty BM, O'Leary DH, Tracy RP, Bush TL. The CHS Collaborative Research Group. Associations of postmenopausal estrogen use with cardiovascular disease and its risk factors in older women. Circulation 1993;88(5 Pt 1):2163–2171.
4. Cho SH, Cho SH, Moon H, Lee JA, Suh JK, Chung HS. The changes of vascular tone and morphology of rat thoracic aorta induced by oophorectomy. Korean J Obstet Gynecol 1994;37(7):1381–1388.
5. Park YS, Kang MS, Kim BK, Kim M. The effect of Eisenia bicyclis extracts on bone tissues in ovariectomized rat. J Korean Soc Food Sci Nutr 2013;42(1):33–39.
6. Chang MJ, Kwon KJ, Kim SH. Calcium and phosphorus balance study by soy isoflavone intake in ovariectomized rats. Korean J Nutr 2005;38(1):40–47.
7. Lim HA, Lim JS, Kim JS. Anti-diabetic effect of puerarin isolated from Puerariae radix. Agric Res Bull Kyungpook Natl Univ 2006;24:29–35.
8. Park CO, Kim KS, Ji YA, Ryu BH. Antioxidant activity of daidzin and puerarin toward oxidation of human low density lipoprotein. J Korean Soc Food Sci Nutr 1997;26(1):25–31.
9. Lee O. Effects of supplementation of Puerariae radix ethanol extract on the antioxidative defense system in rats. Korean J Nutr 2004;37(10):872–880.
10. Hu W, Zhang Q, Yang X, Wang Y, Sun L. Puerarin inhibits adhesion molecule expression in TNF-α -stimulated human endothelial cells via modulation of the nuclear factor κB pathway. Pharmacology 2010;85(1):27–35.
11. Lee SY, Yean MH, Kim JS, Lee JH, Kang SS. Phytochemical studies on Rehmanniae radix. Korean J Pharmacogn 2011;42(2):127–137.
12. You BR, Kim HR, Kim HJ, Lee JY, Lee SY, Song MR, Park JY, Kim MR. Catalpol content and antioxidant activities in various cultivars of Rehmannia glutinosa. J Korean Soc Food Sci Nutr 2011;40(4):481–485.
13. Kim YS, Son YJ, Lee YJ. Effects of Rehmanniae radix on the rat liver damaged with CCl4. Korean J Herbol 2000;15(1):45–51.
14. Kitagawa I, Fukuda Y, Taniyama T, Yoshikawa M. Absolute stereostructures of rehmaglutins A, B, and D three new iridoids isolated from Chinese Rehmanniae radix. Chem Pharm Bull (Tokyo) 1986;34(3):1399–1402.
15. Wang MY, West BJ, Jensen CJ, Nowicki D, Su C, Palu AK, Anderson G. Morinda citrifolia (Noni): a literature review and recent advances in Noni research. Acta Pharmacol Sin 2002;23(12):1127–1141.
16. Damirchi A, Mehdizade R, Ansar MM, Soltani B, Babaei P. Effects of aerobic exercise training on visceral fat and serum adiponectin concentration in ovariectomized rats. Climacteric 2010;13(2):171–178.
17. Pighon A, Barsalani R, Yasari S, Prud'homme D, Lavoie JM. Does exercise training prior to ovariectomy protect against liver and adipocyte fat accumulation in rats? Climacteric 2010;13(3):238–248.
18. Lien EL, Boyle FG, Wrenn JM, Perry RW, Thompson CA, Borzelleca JF. Comparison of AIN-76A and AIN-93G diets: a 13-week study in rats. Food Chem Toxicol 2001;39(4):385–392.
19. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18(6):499–502.
20. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957;226(1):497–509.
21. Yeo E. Menopause, ageing and obesity. J Korean Soc Study Obes 2002;11(3):289–298.
22. Lee SK. Effect of 17-beta estradiol on adipocyte lipin-1 expression in OLETF rat. Endocrinol Metab 2010;25(3):177–179.
23. Dubuc PU. Effects of estrogen on food intake, body weight, and temperature of male and female obese mice. Proc Soc Exp Biol Med 1985;180(3):468–473.
24. Kahn BB, Flier JS. Obesity and insulin resistance. J Clin Invest 2000;106(4):473–481.
25. Fadel L. Conservation role of vitamin K in disturbance glucose homeostasis in rat ovaries and actions exercised. Bull Appl Res Sci 2014;4(2):17–25.
26. Hulver MW, Zheng D, Tanner CJ, Houmard JA, Kraus WE, Slentz CA, Sinha MK, Pories WJ, MacDonald KG, Dohm GL. Adiponectin is not altered with exercise training despite enhanced insulin action. Am J Physiol Endocrinol Metab 2002;283(4):E861–E865.
27. Cnop M, Havel PJ, Utzschneider KM, Carr DB, Sinha MK, Boyko EJ, Retzlaff BM, Knopp RH, Brunzell JD, Kahn SE. Relationship of adiponectin to body fat distribution, insulin sensitivity and plasma lipoproteins: evidence for independent roles of age and sex. Diabetologia 2003;46(4):459–469.