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Abstract
This study was conducted in order to investigate the effects of Artemisia capillaris (AC) extract on disorders of hepatic functions and lipid metabolism induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an endocrine disrupter, using male rats (SD, five weeks old) for a period of three weeks. These 37 animals were divided into four groups. AC extract was added as 1.5% or 3% levels to basal diets, respectively. TCDD (40 ug/kg B.W) was administered by intraperitoneal injection into rats after a week from the beginning of the experiment. AC extract alleviated the increase of rat's relative liver weights induced by TCDD. Thymuses of all rats treated with TCDD were apparently shrunken by approximately 80%. Levels of white blood cells (WBC), red blood cells, hemoglobin, and hematocrits were significantly increased by treatment with TCDD, however, WBC tended to decrease by AC extract diets. In hepatic function, the elevation of glutamic oxalacetic transaminase activities by TCDD treatment was diminished by AC extract diets. Serum HDL-cholesterol levels were significantly elevated by AC extract diets. The apparent increase of triglyceride levels of rat livers induced by TCDD was significantly suppressed in the AC extract diet groups. Hepatic cytosolic catalase activities significantly decreased by treatment with TCDD showed a recovering trend by AC extract diets. In histochemical observation, the fat droplets and apoptosis of hepatocytes treated with TCDD were markedly alleviated by AC extract diets. These results indicated that AC could exert recovering effects on some disorders of hepatic functions, lipids metabolism, and antioxidant activities resulting from TCDD treatment.
Figures and Tables
Fig. 1
Effects of Artemisia capillaris on liver lipids in rats treated 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD). Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment. Mean ± SD of 9-10 rats per group. Values in a column with different superscripts are significantly different (p < 0.05).
Fig. 2
Effects of Artemisia capillaris on hepatic cytosolic superoxide dismutase (SOD) and catalase in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment. Mean ± SD of 9-10 rats per group. Values in a column with different superscripts are significantly different (p < 0.05).
Fig. 3
Effects of Artemisia capillaris on hepatic malondialdehyde (MDA) in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment. Mean ± SD of 9-10 rats per group. Values in a column with different superscripts are significantly different (p < 0.05).
Fig. 4
Effects of Artemisia capillaris on ethoxycoumarine-o-deethylase (ECOD) and Benzphetamine N-demethylase (BPND) in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment. Mean ± SD of 9-10 rats per group. Mean ± SD of 9-10 rats per group. Values in a column with different superscripts are significantly different (p < 0.05).
Fig. 5
Effects of Artemisia capillaris in micrograph of hepatic histology in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)(×200). Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment.
Table 2
Effects of Artemisia capillaris on body weight and food intake in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
1) Mean ± SD of 9-10 rats per group 2) Values in a column with different superscripts are significantly different (p < 0.05). FER: food efficiency ratio; weight gain (g) / food intake (g)
Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment
Table 3
Effects of Artemisia capillaris on organ weights in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
1) Mean ± SD of 9-10 rats per group 2) Values in a column with different superscripts are significantly different (p < 0.05)
Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment
Table 4
Effects of Artemisia capillaris on hematological values in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
1) Mean ± SD of 9-10 rats per group 2) Values in a column with different superscripts are significantly different (p < 0.05)
Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment. RBC: red blood cells, WBC: white blood cells, Hb: hemoglobin, Hct: hematocrits, PLT: platelets
Table 5
Effects of Artemisia capillaris on serum GOT, GPT and ALP in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
1) Mean ± SD of 9-10 rats per group 2) Values in a column with different superscripts are significantly different (p < 0.05)
Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment, GOT: glutamic oxalacetic transaminase, GPT: glutamic pyruvic transaminase, ALP: alkaline phosphatase
Table 6
Effects of Artemisia capillaris on serum lipids in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
1) Mean ± SD of 9-10 rats per group 2) Values in a column with different superscripts are significantly different (p < 0.05)
Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment
Table 7
Effects of Artemisia capillaris on fecal output and lipids in rats treated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
1) Mean ± SD of 9-10 rats per group 2) Values in a column with different superscripts are significantly different (p < 0.05)
Control: basal diet, TCDD: basal diet and TCDD treatment, AC-1.5T: 1.5% Artemisia capillaris was added to basal diet and TCDD treatment, AC-3.0T: 3.0% Artemisia capillaris was added to basal diet and TCDD treatment
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