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Abstract
Background
Infection by the intracellular bacteria Mycoplasma pneumoniae, Chamydophila pneumoniae, and Legionella pneumophila are common causes of community-acquired pneumonia (CAP). This study describes the evaluation of a new multiplex realtime PCR test, EuDx TM-PN MLC Detection Kit (EUDIPIA), which allows the simultaneous detection of M. pneumoniae, C. pneumoniae, and L. pneumophila in respiratory samples.
Methods
A total of 353 samples were tested using three PCR kits: multiplex PCR (Seeplex PneumoBacter ACE Detection Kit) and two multiplex real-time PCR (EuDx TM-PN MLC Detection Kit and Anyplex TM II RB5 Detection Kit). The results were considered true positives (expanded standard) for M. pneumoniae, C. pneumoniae, and L. pneumophila if they were positive according to any of the three tests.
Results
The sensitivity and specificity of EuDx TM-PN MLC Detection Kit were 93.3–100% and 100%, respectively. The agreement rate and Cohen's kappa coefficient (value) between EuDx TM-PN MLC Detection Kit and Anyplex TM II RB5 Detection Kit for M. pneumoniae, C. pneumoniae, and L. pneumophila were 70-100% and 0.82–1, respectively.
References
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Table 1.
Sensitivity and specificity of the tests for Mycoplasma pneumoniae to extended standard
Table 2.
Sensitivity and specificity of the tests for Chamydophila pneumoniae to extended standard
Table 3.
Sensitivity and specificity of the tests for Legionella pneumophila to extended standard
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