Journal List > Ann Clin Microbiol > v.20(4) > 1078581

Ann Clin Microbiol. 2017 Dec;20(4):97-102. Korean.
Published online December 20, 2017.  https://doi.org/10.5145/ACM.2017.20.4.97
© 2017 The Korean Society of Clinical Microbiology
Evaluation of EuDx™-PN MLC Detection Kit for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila in Respiratory Specimens
Mi-Kyung Lee,1 Heungsup Sung,2 Ah Ra Cho,3 and Hyun Young Chi4
1Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
2Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
3Seoul Clinical Laboratories (SCL), Yongin, Korea.
4Samkwang Medical Laboratories, Seoul, Korea.

Correspondence: Mi-Kyung Lee, Department of Laboratory Medicine, Chung-Ang University Hospital, 102 Heukseok-ro, Dongjak-gu, Seoul 06973, Korea. (Tel) 82-2-6299-2719, (Fax) 82-2-6298-8630, Email: cpworld@cau.ac.kr
Received July 19, 2017; Revised August 26, 2017; Accepted September 02, 2017.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Background

Infection by the intracellular bacteria Mycoplasma pneumoniae, Chamydophila pneumoniae, and Legionella pneumophila are common causes of community-acquired pneumonia (CAP). This study describes the evaluation of a new multiplex real-time PCR test, EuDx™-PN MLC Detection Kit (EUDIPIA), which allows the simultaneous detection of M. pneumoniae, C. pneumoniae, and L. pneumophila in respiratory samples.

Methods

A total of 353 samples were tested using three PCR kits: multiplex PCR (Seeplex PneumoBacter ACE Detection Kit) and two multiplex real-time PCR (EuDx™-PN MLC Detection Kit and Anyplex™ II RB5 Detection Kit). The results were considered true positives (expanded standard) for M. pneumoniae, C. pneumoniae, and L. pneumophila if they were positive according to any of the three tests.

Results

The sensitivity and specificity of EuDx™-PN MLC Detection Kit were 93.3–100% and 100%, respectively. The agreement rate and Cohen's kappa coefficient (value) between EuDx™-PN MLC Detection Kit and Anyplex™ II RB5 Detection Kit for M. pneumoniae, C. pneumoniae, and L. pneumophila were 70–100% and 0.82–1, respectively.

Conclusion

These results demonstrate that the EuDx™-PN MLC Detection Kit is a sensitive, specific, and useful screening tool for the detection of atypical pathogens in respiratory samples and can be helpful in selecting appropriate antimicrobial therapy for patients with respiratory infection.

Keywords: Chamydophila pneumoniae; Legionella pneumophila; Real-time polymerase chain reaction; Mycoplasma pneumoniae; Respiratory tract infection

Tables


Table 1
Sensitivity and specificity of the tests for Mycoplasma pneumoniae to extended standard
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Table 2
Sensitivity and specificity of the tests for Chamydophila pneumoniae to extended standard
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Table 3
Sensitivity and specificity of the tests for Legionella pneumophila to extended standard
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Table 4
Comparison of EuDx™-PN MLC Detection Kit and Anyplex™II RB5 Detection Kit for the detection of Mycoplasma pneumoniae, Chamydophila pneumoniae, and Legionella pneumophila
Click for larger image

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