Rapid Detection of Group B Streptococcus Using ChromID STRB and PCR in the Pregnant Women

Dong-Hyun Lee; Hyoshim Shin; Sunjoo Kim

doi:10.5145/ACM.2017.20.4.103

Journal List > Ann Clin Microbiol > v.20(4) > 1078566

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Lee, Shin, and Kim: Rapid Detection of Group B Streptococcus Using ChromID STRB and PCR in the Pregnant Women

Original Article

Ann Clin Microbiol 2017;20(4):103-108.

Published online: 12 December 2017

DOI: https://doi.org/10.5145/ACM.2017.20.4.103

Rapid Detection of Group B Streptococcus Using ChromID STRB and PCR in the Pregnant Women

Dong-Hyun Lee¹, Hyoshim Shin¹, Sunjoo Kim^1,²

^¹Department of Laboratory Medicine, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Sciences, Jinju

^²Department of Laboratory Medicine, Changwon Gyeongsang National University Hospital, Changwon, Korea

Correspondence: Sunjoo Kim, Department of Laboratory Medicine, Changwon Gyeongsang National University Hospital, 11 Samjungja-ro, Seongsan-gu Changwon 51472, Korea. (Tel) 82-55-214-3072, (Fax) 82-55-214-3087, (E-mail) sjkim8239@hanmail.net

Received 27 September 2017 Revised 2 November 2017 Accepted 8 November 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Group B Streptococcus (GBS) can be transmitted to neonates during delivery through the birth canal. As awareness of neonatal GBS infections is increasing, more rapid and efficient screening tests are required. The aim of this study was to evaluate the performance of ChromID STRB (bioMérieux, France) and PCR compared with the standard culture method.

Methods

Recto-vaginal swabs were collected from 775 pregnant women from April 2016 to March 2017. Cotton swab cultures were grown in LIM broth overnight and then subcultured onto blood agar plates and ChromID STRB. PCR was carried out to detect atr genes specific for GBS.

Results

The carrier rate of GBS was 5.9% (46/775). The sensitivity, specificity, positive predictive value, and negative predictive value were 83.8%, 99.3%, 86.1%, and 99.2%, respectively, for ChromID STRB and 89.2%, 99.6%, 91.7%, and 99.5%, respectively for PCR. Both ChromID STRB and PCR detected 6 more cases compared to the standard culture.

Conclusion

Chromogenic agar, ChromID STRB, and PCR using the atr gene showed excellent performance to screen for GBS. To administer prophylactic antibiotics efficiently, either selective chromogenic agar or PCR could be used in addition to the standard culture.

Keywords: Group B Streptococcus, Infection, Pregnancy, Streptococcus agalactiae

References

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Table 1.

Primer sequences that were used in PCR for GBS detection

Amplified gene	Primer sequence (5′-3′)	Size (bp)
atr Forward	CAA CGA TTC TCT CAG CTT TGT TAA	780
Reverse	TAA GAA ATC TCT TGT GCG GAT TTC

Table 2.

Combination of the results of each screening test of GBS

Standard culture	PCR	ChromID STRB	N
Positive	Positive	Positive	27
Positive	Positive	Negative	6
Positive	Negative	Positive	4
Negative	Positive	Positive	3
Positive	Negative	Negative	0
Negative	Positive	Negative	3
Negative	Negative	Positive	3
Negative	Negative	Negative	729
	Total		775

Table 3.

Performance of ChromID STRB and PCR test compared to standard culture (95% confidence interval)

Parameters	ChromID STRB	PCR
Sensitivity (%)	83.9 (68.0–93.8)	89.2 (74.6–97.0)
Specificity (%)	99.3 (98.4–99.8)	99.6 (98.8–99.9)
Positive predictive value (%)	86.1 (71.9–93.8)	91.7 (78.0–97.2)
Negative predictive value (%)	99.2 (98.3–99.6)	99.5 (98.7–99.8)
P value*	1.00	0.75
Turn-around time (hours)^†	^†18–48	6–8

*Compared with standard culture method;

^† TAT does not include primary culture in the LIM broth.

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