Journal List > Ann Clin Microbiol > v.19(3) > 1078558

Kim, Yong, In, Park, and Lee: Application of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry to Screen the Extended-Spectrum β-Lactamase-Producing ST131 Escherichia coli Strains

Abstract

Background

Sequence type 131 (ST131) O25b serogroup Escherichia coli, producing CTX-M type ex-tended-spectrum β-lactamase (ESBL), is a major clone involved in worldwide pandemic spread in both community- and healthcare-associated infections. Recently, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become a routine tool for the identification of bacteria in many laboratories. This study aimed to assess the performance of MALDI-TOF MS for the screening of ESBL-producing E. coli ST131 in a rapid, inexpensive, and simple way.

Methods

A total 26 clinical E. coli, isolated from blood between 2013 and 2014, were used. The characteristics are ST131-O25b ESBL producers (n=6), ST131-O16 ESBL producers (n=4), non-ST131 ESBL producers (n=11), and non-ST131 non-ESBL producers (n=5). Specific biomarker peaks to distinguish the ST131 clonal group from others were investigated by MicroIDSys (ASTA, South Korea) and ASTA Tinker-bell 2.0 software.

Results

A peak at 9,713 m/z peak is useful to screen for ST131 E. coli, regardless of serogroup O25 or O16, showing a sensitivity of 100%, specificity of 56.2%, positive predictive value of 58.8%, and negative predictive value of 100% when using a relative intensity cutoff of 15%.

Conclusion

We can screen for ST131 E. coli using MicroIDSys (ASTA), MALDI-TOF MS in a rapid, inex-pensive, and simple way. However, other confirmatory tests are needed to confirm ST131 E. coli due to the low specificity of this method.

References

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Fig. 1.
Visual inspection of 9,713 m/z for ST131 E. coli with relative intensity 15% (cutoff). Test performance: sensitivity 100%, specificity 56.2%, positive-predictive value 58.8%, and negative-predictive vale 100%.
acm-19-65f1.tif
Table 1.
Characteristics of the 26 Escherichia coli isolates, included in this study
Group N ESBL (n) MLST
PST (n) ST (n)
Non-ST131-ESBL 11 CTX-M-1 (1), CTX-M-14 (3), CTX-M-15 (6), CTX-M-27 (1) PST2 (1), PST3 (1), PST8 (2), PST13 (1), PST39 (1), PST44 (1), PST53 (1), PST253 (1), PST478 (1), PST594 (1)  
Non-ST131-NonESBL 5 None    
ST131-O16-ESBL 4 CTX-M-14 (2), CTX-M-15 (1), CTX-M-27 (1) PST506 (4) ST131 (4)
ST131-O25-ESBL 6 CTX-M-14 (2), CTX-M-15 (4) PST43 (6) ST131 (6)
Total 26      

Abbreviations: n, number; ESBL, extended-spectrum β-lactamase; MLST, multilocus sequence typing; PST, Pasteur method; ST, Achtman method.

Table 2.
The peaks for the detection of ST131 Escherichia coli and their performances
m/z Cutoff (Relative intensity) Positive rate (%) Marker for:
Non-ST131 ST131-O16 ST131-O25
3,580 9 31.3 100.0 16.7 ST131-O16
3,935 6 43.8 100.0 33.3 ST131-O16
5,382 60 31.3 100.0 16.7 ST131-O16
6,256 36 31.3 100.0 16.7 ST131-O16
6,316 25 37.5 100.0 16.7 ST131-O16
6,506 6 37.5 100.0 33.3 ST131-O16
7,159 6 43.8 100.0 16.7 ST131-O16
8,350 5 37.5 50.0 100.0 ST131-O25
8,993 7 43.8 100.0 33.3 ST131-O16
9,536 17 37.5 100.0 50.0 ST131-O16
9,713 15 43.8 100.0 100.0 ST131-O16 & O25

Cutoff 0.15=relative intensity 15%.

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