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Park, Huh, Ha, Kim, Ki, and Lee: A Case of Bacteremia Caused by Dialister pneumosintes with Streptococcus anginosus
Original Article

Ann Clin Microbiol 2015;18(2):60-63.

Published online: 5 June 2015

DOI: https://doi.org/10.5145/ACM.2015.18.2.60

A Case of Bacteremia Caused by Dialister pneumosintes with Streptococcus anginosus

Jong Eun Park1, Hee Jae Huh1, Young Eun Ha2, Wook Sung Kim3, Chang-Seok Ki1, Nam Yong Lee1

1Department of Laboratory Medicine and Genetics, Seoul, Korea

2Division of Infectious Diseases, Department of Medicine, Seoul, Korea

3Department of Thoracic and Cardiovascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

Correspondence: Nam Yong Lee, Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 135-710, Korea. (Tel) 82-2-3410-2706, (Fax) 82-2-3410-2719, (E-mail) micro.lee@samsung.com

Received 3 February 201510 March 2015       Accepted 11 March 2015

Copyright © 2015 The Korean Society of Clinical Microbiology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

초록

Dialister pneumosintes is a nonfermentative, gram-negative anaerobic rod which is considered as a commen-sal organism of the oral cavity. A 77-year-old man with a history of aortic stenosis was visited to ER for dyspnea and fever. D. pneumosintes and Streptococcus anginosus were isolated from blood culture, and also D. pneumosintes was identified by 16S rRNA-based gene sequencing. This case report is the first case of isolation of D. pneumosintes from blood in Korea, and highlights the usefulness of DNA sequencing to identify pathogens in organism which is difficult to identify by biochemical identification method.

Keywords: Dialister pneumosintes, Infective endocarditis, Sequencing, 16S rRNA

REFERENCES

1.Olitsky PK., Gates FL. Experimental studies of the nasophar-yngeal secretions from influenza patients: IV. anaerobic cultivation. J Exp Med. 1921. 33:713–29.
2.Willems A., Collins MD. Phylogenetic placement of Dialister pneumosintes (formerly Bacteroides pneumosintes) within the Sporomusa subbranch of the Clostridium subphylum of the gram-positive bacteria. Int J Syst Bacteriol. 1995. 45:403–5.
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Fig. 1.
Molecular phylogenetic tree constructed by neighbor-joining method using the 16s rRNA sequences of our case and related species. Reference sequences were from the type strains of the species and accession numbers are given in parentheses. All names and accession numbers are given as cited in the GenBank database.
acm-18-60f1.tif
Table 1.
Dialister pneumosintes infections excluding dental disease
Year Age/Sex Specimen Isolate Identification method Clinical information Treatment Outcome
2002[6] 17/M Blood Dialister pneumosintes, Streptococcus anginosus 16S rRNA sequence analysis Subdural empyema CTX, MTZ, ACV Improved
2002[6] 66/M Brain abscess Dialister pneumosintes 16S rRNA sequence analysis Brain abscess CTX, MTZ, FOS Improved
2006[3] 27/F Blood Dialister pneumosintes 16S rRNA sequence analysis Post-partum with vaginosis IPM, RIF Improved
2010[10] 74/M Bronchoscopy specimen Dialister pneumosintes Not described Pneumonia AMC Improved
This case 77/M Blood Dialister pneumosintes, Streptococcus anginosus 16S rRNA sequence analysis Infective endocarditis CTR, MTZ Died

Abbreviations: CTX, cefotaxime; MTZ, metronidazole; ACV, acyclovir; FOS, fosfomycin; IPM, imipenem; RIF, rifampin; AMC, amoxicillin-clavulanate; CTR, ceftriaxone.

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