Detection of Respiratory Viruses and Atypical Bacterial Pathogens in Infants with Acute Respiratory Infections Using Multiplex PCR

Soo Jin Yoo; Jeong-U Han; Bo-Moon Shin

doi:10.5145/ACM.2014.17.3.86

Journal List > Ann Clin Microbiol > v.17(3) > 1078517

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Yoo, Han, and Shin: Detection of Respiratory Viruses and Atypical Bacterial Pathogens in Infants with Acute Respiratory Infections Using Multiplex PCR

Original Article

Ann Clin Microbiol 2014;17(3):86-90.

Published online: 5 September 2014

DOI: https://doi.org/10.5145/ACM.2014.17.3.86

Detection of Respiratory Viruses and Atypical Bacterial Pathogens in Infants with Acute Respiratory Infections Using Multiplex PCR

Soo Jin Yoo, Jeong-U Han, Bo-Moon Shin

Department of Laboratory Medicine, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea

Correspondence: Bo-Moon Shin, Department of Laboratory Medicine, Sanggye Paik Hospital, Inje University College of Medicine, 761-1, Sanggye 7-dong, Nowon-gu, Seoul 139-707, Korea. (Tel) 82-2-950-1227, (Fax) 82-2-950-1244, (E-mail) bmshin@unitel.co.kr

Received 31 March 201423 May 2014 Accepted 11 June 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

초록

Multiplex PCR of nasopharyngeal aspirates detected viruses and atypical bacteria in 75.3% (219/291) of infants with acute respiratory infections from July 2010 to June 2013. Frequent viruses were human rhinovirus (29.9%), parainfluenza virus (21.7%), respiratory syncytial virus (17.9%), and human metapneumovirus (10.3%). Mycoplasma pneumoniae and Bordetella pertussis were detected in 3.4% and 0.3%, respectively. (Ann Clin Microbiol 2014;17:86-90)

Keywords: Infant, Multiplex PCR, Respiratory infection

REFERENCES

1.Johansson N., Kalin M., Tiveljung-Lindell A., Giske CG., Hedlund J. Etiology of community-acquired pneumonia: increased microb-iological yield with new diagnostic methods. Clin Infect Dis. 2010. 50:202–9.

2.Honkinen M., Lahti E., Österback R., Ruuskanen O., Waris M. Viruses and bacteria in sputum samples of children with community- acquired pneumonia. Clin Microbiol Infect. 2012. 18:300–7.

3.Yoo SJ., Kuak EY., Shin BM. Detection of 12 respiratory viruses with two-set multiplex reverse transcriptase-PCR assay using a dual priming oligonucleotide system. Korean J Lab Med. 2007. 27:420–7.

4.Ham H., Jang J., Choi S., Oh S., Jo S., Choi S, et al. Epidemiological characterization of respiratory viruses detected from acute respiratory patients in Seoul. Ann Clin Microbiol. 2013. 16:188–95.

5.Kim JW., Seo HK., Yoo EG., Park SJ., Yoon SH., Jung HY, et al. Mycoplasma pneumoniae pneumonia in Korean children, from 1979 to 2006-a meta-analysis. Korean J Pediatr. 2009. 52:315–23.

6.Song JH., Oh WS., Kang CI., Chung DR., Peck KR., Ko KS, et al. Asian Network for Surveillance of Resistant Pathogens Study Group. Epidemiology and clinical outcomes of community-acqu-ired pneumonia in adult patients in Asian countries: a prospective study by the Asian network for surveillance of resistant pathogens. Int J Antimicrob Agents. 2008. 31:107–14.

7.Song JH., Thamlikitkul V., Hsueh PR. Clinical and economic burden of community-acquired pneumonia amongst adults in the Asia- Pacific region. Int J Antimicrob Agents. 2011. 38:108–17.

8.Kim YN., Park CH., Kim SH., Yoo SJ., Kim HB. Factors associated with various clinical manifestations of Mycoplasma pneumonia in children. Allergy Asthma Respir Dis. 2013. 1:357–61.

9.Choe YJ., Park YJ., Jung C., Bae GR., Lee DH. National pertussis surveillance in South Korea 1955-2011: epidemiological and clinical trends. Int J Infect Dis. 2012. 16:e850–4.

10.Kwon HJ., Yum SK., Choi UY., Lee SY., Kim JH., Kang JH. Infant pertussis and household transmission in Korea. J Korean Med Sci. 2012. 27:1547–51.

Fig. 1.

Monthly distributions of frequent viruses and Mycoplasma pneumonia infections in infants with acute respiratory symptoms from July 2010 to June 2013.

Table 1.

PCR results and monthly distributions for respiratory pathogens in 291 infants with acute respiratory symptoms

Pathogens	No. of detection	*%	Year 2010		2011				2012				2013
			Month	7-9	10-12	1-3	4-6	7-9	10-12	1-3	4-6	7-9	10-12	1-3	4-6
			N	32	41	10	41	20	17	12	45	4	7	19	43
Positive rate, %				72.7	73.2	70.0	78.0	50.0	82.4	66.7	73.3	100	100	78.9	86.0
Human rhinovirus	87	29.9%		9	10	4	11	2	4	2	20	4	2	3	16
Parainfluena virus	63	21.7%
Parainfluena virus 3	46	15.8%		4			10	2	1	1	12				16
Parainfluena virus 1	15	5.2%		3			3	1	2		2			1	3
Parainvluena virus 2	2	0.7%		3			3	1	2		2			1	3
Respiratory syncytial virus	52	17.9%
Respiratory syncytial virus B	28	9.6%		4	16	4	2	1					1
Respiratory syncytial virus A	24	8.2%					1	3	10	1		1	3	4	1
Human metapneumovirus	30	10.3%		1		1	9	2		2	1			6	5
Adenovirus	28	9.6%		5	3		3		1		4		1	1	10
Human coronavirus	16	5.5%
Human coronavirus	13		4.5%	3	5				2	1				1	1
229E/NL63
Human coronavirus	3	1.0%								1	2
OC43
Human bocavirus†	14	4.8%		-	-	-	-	-	-	-	-	-	-	1	13
Mycoplasma pneumoniae	10	3.4%		1	3		2	1	1	1					1
Influenza virus A	6	2.1%							1	3				1	1
Human enterovirus†	3	1.0%		-	-	-	-	-	-	-	-	-	-		3

^∗ Coinfections included in each pathogens;

^† Human bocavirus and enterovirus were tested from Feb. 2013 and positive in 24.6% (14/57) and 5.3% (3/57), respectively.

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