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초록
Background
The prestorage condition of blood culture bottles prior to entering the automated blood culture system may affect the time to detection (TTD) of microorganisms and the final report days.
Methods
We compared the TTD and final report days according to the preincubation conditions after laboratory operating hours: room temperature (RT) vs. a BacT/Alert unit (BioMerieux Inc.) for 3 months respectively. All bottles were inserted into the main BacT/Alert system the next morning.
Results
TTD was significantly reduced by preincubating bottles in a BacT/Alert unit (median, 8.4 h) compared to prestorage at RT (median, 12.4 h) (P＜ 0.001). The final report of bacterial identification and drug susceptibility within 2 days was available for 24.4% of bottles preincubated in a BacT/Alert unit compared to 14.9% of those incubated at RT. The false positive results were significantly higher for preincubation in a BacT/Alert unit (0.81%) than for that (0.29%) at RT (P＜0.001).
Conclusion
If a clinical microbiology laboratory is not operational for 24 hours, an automated blood culture unit might be a good alternative to reduce TTD and allow the submission of a faster final report compared to prestorage at RT. However, false positive readings increased more than two-fold by preincubation in a BacT/Alert unit.
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Fig. 1.
Comparison of time to detection (TTD) of (A) all microorganisms, (B) Gram (+) organisms, (C) Gram (-) organisms, and (D) yeasts according to prestorage of blood culture bottles at room temperature and in a BacT/Alert unit outside laboratory operating hours. P＜0.001 in (A), (B), and (C) by Mann-Whitney U test.
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