Journal List > J Korean Breast Cancer Soc > v.7(4) > 1076737

J Korean Breast Cancer Soc. 2004 Dec;7(4):228-235. Korean.
Published online December 31, 2004.  https://doi.org/10.4048/jkbcs.2004.7.4.228
Copyright © 2004 Korean Breast Cancer Society
Correlation of Immunohistochemical Expression of MDR1, MRP1, Topoisomerase IIalpha with Prognostic Factors and Histoculture Drug Response Assay (HDRA) Result in Breast Carcinoma
Hee Joon Kang,1 Sung Hee Hong,2 Byung Ho Son, Ho Sung Yoon,3 Gyung Yub Gong,4 and Sei Hyun Ahn
Department of Surgery, Ulsan University College of Medicine, Korea.
1Department of Surgery, Hallym University College of Medicine, Korea.
2Hong Sung Hee Women Clinic, Korea.
3Department of Surgery, Hanyang University College of Medicine, Korea.
4Department of Pathology, Ulsan University College of Medicine, Korea.
Received October 12, 2004; Accepted November 18, 2004.

Abstract

Purpose

Drug resistance plays an important role in the failure of chemotherapy in breast cancer. The purpose of the study was to investigate the chemosensitive and chemoresistance indices of breast carcinomas and see if the in vitro chemosensitivity test correlated with the prognostic indices.

Methods

The immunohistochemical expressions of MDR1, MRP1 and topoisomerase IIα (topo IIα) were studied and then correlated these with the in vitro chemosensitivities using the histoculture drug response assay (HDRA) and clinicopathological factors in 51 breast carcinomas.

Results

In the breast carcinomas examined, the immunohistochemical expressions of MDR1, MRP1 and topo IIα were strongly observed in 26 (51.0%), 16 (32.0%), 15 (31.3%) carcinomas, respectively. The MRP1 was more frequently expressed in poorly differentiated carcinomas (P=0.006), and those of MDR1 and topo IIα were more frequently observed in tumor overexpressing cerbB2 (P=0.038, P=0.036). The expression of MDR1 was related to that of topo IIα (P=0.015). Comparing these markers with the in vitro chemosensitivities to cyclophosphamide, 5-FU, adriamycin, taxol and taxotere, no correlations were found between the expression of MDR1, MRP1, and topo IIα but from the chemosensitivity using the HDRA, the growth inhibition rate for cyclophosphamide was higher in MRP1 expressing carcinomas (P=0.009).

Conclusion

MDR1, MRP1 and topo IIα were all found to be associated with the poor prognostic indices, but assessment of their immunohistochemical expressions did not allow for prediction of the response to chemotherapy by the in vitro chemosensitivity test in breast carcinomas.

Keywords: Breast cancer; Prognostic factor; Chemoresistance; Chemosensitivity; HDRA; MDR1; MRP1; Topoisomerase IIα