Eun Jung Lee; Tae Hyong Kim; Sung-Han Kim; Ji Hyun Kim; Bum Jin Oh; Yang Soo Kim; Kyung Soo Lim; Jun Hee Woo

doi:10.3947/ic.2009.41.2.90

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Lee, Kim, Kim, Kim, Oh, Kim, Lim, and Woo: Rapid Diagnosis of Human Immunodeficiency Virus Infection Using OraQuickⓡ Advance Rapid HIV-1/2 Antibody Test

Original Article

Infection and Chemotherapy

Infection and Chemotherapy 2009; 41(2): 90-94.

Published online: 30 April 2009

DOI: https://doi.org/10.3947/ic.2009.41.2.90

Rapid Diagnosis of Human Immunodeficiency Virus Infection Using OraQuickⓡ Advance Rapid HIV-1/2 Antibody Test

Eun Jung Lee, M.D.¹, Tae Hyong Kim, M.D., Ph.D.³

, Sung-Han Kim, M.D., Ph.D.¹, Ji Hyun Kim, R.N.¹, Bum Jin Oh, M.D., Ph.D.², Yang Soo Kim, M.D., Ph.D.¹, Kyung Soo Lim, M.D., Ph.D.², Jun Hee Woo, M.D., Ph.D.¹

¹Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

²Department of Emergency Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

³Department of infectious Diseases, SoonChunHyang University, Seoul, Korea.

Correspondence: June Hee Woo, M.D., Ph.D. Department of Infectious Diseases, Asan Medical Center, University of Ulsan, College of Medicine. 388-1 Pungnap-2dong, Songpa-gu, Seoul, 138-736, Korea. Tel: +82-2-3010-3302, Fax: +82-2-3010-6970, thanks1126@ paran.com

Received 27 November 2008 Accepted 24 February 2009

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

From medical care perspective regarding HIV, early detection of HIV is critical in effectively managing its sequelae. People who are aware of the fact that they are infected with HIV significantly reduce high risk behaviors, thus limiting transmission to others compared to individuals who are unaware of their HIV serostatus.

Materials and Methods

HIV rapid test validation study was conducted at Asan medical center using OraQuick Advance Rapid HIV-1/2 Antibody Test which uses oral fluid specimens for the diagnosis of HIV infection. A total of 57 subjects were tested; 53 individuals were confirmed to have HIV infection by the Western blot analysis and 4 individuals were negative for HIV.

Results

The oral fluid specimens from 52 of 53 infected individuals showed positive reaction to the OraQuick Advance Rapid HIV-1/2 Antibody Test. The test results were negative for the 4 subjects who were negative for HIV. OraQuick Advance Rapid HIV-1/2 Antibody Test's sensitivity was 98.1%, specificity was 100%, positive predictive value was 100%, and negative predictive value was 80%.

Conclusion

The recently approved OraQuick Advance Rapid HIV-1/2 Antibody Test has the ability to detect HIV antibodies comparable to that of confirmatory tests such as Western blot analysis.

Keywords: OraQuick Rapid HIV-1/2 Antibody Test, HIV rapid diagnosis

Figures and Tables

Figure 1

The result of OraQuickⓡ Advance Rapid HIV-1/2 Antibody Test shows negative reaction at the T zone A), but positive reaction (red line at the T zone) B).

Table 1

Demographic Characteristics of Enrolled Patients with HIV (n=53)

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ORCID iDs: Tae Hyong Kim
https://orcid.org/http://orcid.org/0000-0003-2920-9038
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