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Han, Kim, Kim, and Kim: Serological Kinetics of Adenylate Kinase 3 in Rat Acute Myocardial Infarction
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Korean Circulation Journal

Korean Circulation Journal 2004; 34(3): 288-295.

Published online: 31 March 2004

DOI: https://doi.org/10.4070/kcj.2004.34.3.288

Serological Kinetics of Adenylate Kinase 3 in Rat Acute Myocardial Infarction

Jae Seung Han, Hyo Joon Kim, Hyuck Kim, Kyung Soo Kim

Division of Molecular Immunology, Department of Biochemistry

Department of Thoracic Surgery, College of Medicine, Hanyang University, Seoul, Korea.

Division of Cardiology, Department of Internal Medicine, College of Medicine, Hanyang University, Seoul, Korea.

Copyright © 2004 The Korean Society of Circulation

Abstract

BACKGROUND AND OBJECTIVES: Currently used serological markers for the diagnosis of acute myocardial infarction differ in appearance time and specificity for myocardial infarction, allowing no ideal single serological marker for myocardial infarction. Adenylate kinase (AK) is a ubiquitous enzyme which contributes to the homeostasis of the cellular adenine nucleotides pool. AK is abundant in the myocardium, and we postulated that AK3 could be used as a biochemical marker for the diagnosis of acute myocardial infarction (AMI).
MATERALS AND METHODS: We constructed an AMI rat model with ligation of the anterior descending artery. We measured the concentration of serum AK3 in the AMI rat model by enhanced chemiluminescence (ECL) sandwich ELISA using monoclonal antibodies against recombinant AK3.
RESULTS: The serum AK3 level started to increase in 3 hours and reached a peak at 6 hours after ligation of the rat coronary artery. The significant elevation of AK3 was retained for 2 days (p<0.05).
CONCLUSION: AK3 is a useful serological marker for acute myocardial infarction in the rat.

Keywords: Myocardial infarction/enzymology, Adenylate kinase

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