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Yoon, Park, and Park: Effect of Glutathione on Oxidant-induced Cardiotoxicity
Original Article
Korean Circulation Journal

Korean Circulation Journal 1997; 27(4): 433-437.

Published online: 30 April 1997

DOI: https://doi.org/10.4070/kcj.1997.27.4.433

Effect of Glutathione on Oxidant-induced Cardiotoxicity

Hyang-Suk Yoon, M.D., Seung-Taeck Park, Ph.D.*, Ock-Kyu Park, M.D.**

Department of Pediatrics, Wonkwang University School of Medicine, Iksan, Korea.

*Department of Anatomy, Wonkwang University School of Medicine, Iksan, Korea.

**Division of Cardiology, Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Korea.

Copyright © 1997 The Korean Society of Circulation

Abstract

BACKGROUND

In order to elucidate toxic mechanism of the oxygen radicals on cultured rat myocardial cells, cytotoxic effect of oxygen radicals was evaluated by MTT assay. In addition protective effect of glutathione(GSH) on oxidant-induced cardiotoxicity was investigated on these cultures.

METHODS

Myocardial cells derived from neonatal rats were cultured for 12 hours in the medium containing various concentrations of glucose oxidase(GO). Cell viability was measured by MTT assay and morphological changes of the myocardial cells were observed by light microscope.

RESULTS

GO-mediated oxygen radicals remarkably decreased cell viability of cultured myocardial cells in a dose-and time-dependent manner. And also, GSH blicked GO-induced cardiotoxicity in these cultures.

CONCLUSION

These results suggest that the oxygen radicals are tixic and the selective antioxidants such as GSH are effective in blocking against the oxidant-induced cardiotoxicity in cultures of the myocardial cells of neonatsl rats.

Keywords: Cultured myocardial cells, Oxygen radicals, Cardiotoxicity, Antioxidant

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