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Abstract
It has been reported that kanamycin, a useful agent in the treatment of gram-negative and other infectious disease, has a negative inotropic action in isolated cardiac muscle preparation and also it decreases the amplitude of high K+ -induced action potential of guinea pig atrium. These findings imply that kanamycin has a property of Ca2+ antagonist.
In this study, the effects of kanamycin on cardiac Ca2+ current were investigated in isolated rabbit ventricular cells by using a whole cell clamp nethod.
The results are summarized as follows ;
1) Kanamycin caused a depression fo cardiac Ca2+ current in a dose dependent manner and its effect was observed in a whole memberane potential renge.
2) The decreasing effect of kanamycin on Ca2+ current was inhibited by high Ca2+-Tyrode solution and such inhibition was also observed in high Sr2+-Tyrode solution, but in the case of Cd2+, well known inorganic Ca2+antagonist, the current through Ca2+ channel was greatly decreased when the perfuaste was changed from high Ca2+-Tyrode to high Sr2+-Tyrode solution.
3) In the presense of kanamycin, the decreasing pattern of Ca2+ current by repetitve depolarization was not specific as in the case of verapamil.
4) Neomycin decreased Ca2+ current similar to kanamycin.
From the above result, it may be concluded that kanamycin has competitive antagonistin effect on cardiac Ca2+ current.
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