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Abstract
Purpose:
To qualitatively analyse barium coating status in the intestinal mucosa, we used scanning electron microscopy to observe barium particles coated in the small intestinal mucosa of rabbit, and we attempted to assess the relationship between electron microscopic findings and radiographic densities.
Materials and Methods:
Six different combinations of barium and methylcellulose suspensions were infused into the resected small intestines of 15 rabbits. Barium powders were mixed with water to make 40% and 70% w/v barium solutions, and also mixed with 0.5% methylcellulose solution to make 40% and 70% w/v barium-methylcellulose mixtures. 0.5% methylcellulose solutions were used as a double contrast agent. After the infusion of barium suspensions, a mammography unit was used to obtain radiographs of the small intestine, and their optical densities were measured by a densitometer. Thereafter, photographs of barium-coated small intestinal mucosa were obtained using a scanning electron microscope(X 8,000), and the number of barium particles in the unit area were measured. To compare the relationship between the electron microscopic findings and optical densities, statistical analysis using Spearman correlation was performed.
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Fig. 1.
Scanning electron microscopy (SEM) of barium suspension(X 8,000). Barium particles show variable size and shape, but most of them are less than 1 //m. Aggregations are rarely seen.
Fig. 2.
A. SEM of group 1(X 8,000). Most of barium particles are large particles, and small barium particles are rarely seen. B. Radiography of group 1. A radiograph using mammographic technique shows aggregation of barium particles with reticular appearance. C. SEM of group 2(X 8,000). Compared to Fig. 2A, the number of small barium particles are increased. D. Radiography of group 2. Similar reticular patterns are also seen as in group 1. E. SEM of group 3(X 8,000). Small barium particles are densely coated. Particles vary in size and shape, and many particles are aggregated. F. Radiography of group 3. Due to a large amount of barium particles, mucosal details are not seen at all on this very radiopaque radiograph.
Tabel 1.
Experimental Groups and Their Electron Microscopic Findings and Optical Densities
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