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Ryu, Park, and Kim: The Relation of Serum Adiponectin and Resistin Concentrations with Metabolic Risk Factors
Original Article
Journal of Korean Society of Endocrinology

Journal of Korean Society of Endocrinology 2005; 20(5): 444-451.

Published online: 20 October 2005

DOI: https://doi.org/10.3803/jkes.2005.20.5.444

The Relation of Serum Adiponectin and Resistin Concentrations with Metabolic Risk Factors

Seong Tae Ryu, Seok O Park, Se Hwa Kim

Division of Endocrinology, Department of Internal Medicine, Gwangmyung Sung-Ae Hospital, korea.

Received 25 June 2005       Accepted 7 August 2005

Copyright © 2005 Korean Endocrine Society

Abstract

Background

Adiponectin is a fat cell-secreted cytokine, which has been reported to improve insulin sensitivity and have antiatherogenic properties. However, it is still unclear whether resistin plays a significant role in the development of insulin resistance in humans. The aim of this study was to investigate the relationship of the adiponectin and resistin concentrations with insulin resistance, metabolic markers and adiposity in healthy and type 2 diabetic subjects.

Methods

Eighty-three type 2 diabetic and 139 healthy subjects were studied. Blood samples were drawn after fasting to determine the fasting plasma glucose, insulin, resistin, adiponectin, total cholesterol, triglyceride and HDL-cholesterol levels. The subcutaneous and visceral fat areas were measured at the umbilical level using computed tomography.

Results

The serum adiponectin concentrations were significantly lower in the diabetic (6.7 ± 2.3 µg/mL) than in the obese (8.2 ± 2.4 µg/mL, P<0.01) and non-obese subjects (9.9 ± 4.5 µg/mL, P<0.01). The serum resistin concentrations were Similar between the non-obese, obese and type 2 diabetic subjects. From a multiple regression analysis, the fasting glucose, HDL-cholesterol and HOMA-IR were found to be independent determinants of the log of the adiponectin level in the diabetes group. In healthy subjects, the gender, BMI, HOMA-IR, visceral fat area and HDL-cholesterol were associated with the log of the adiponectin level. However, the log of the resistin level was not associated with the markers of insulin resistance and obesity.

Conclusion

This study showed that the serum adiponectin concentration was closely related to the insulin resistance marker in both healthy and type 2 diabetic subjects. However, the resistin concentration was not associated with the markers of insulin resistance and/or obesity.

Keywords: Adiponectin, Resistin, Type 2 Diabetes Mellitus, Obesity, Insulin Resistance

Figures and Tables

Fig. 1
Serum adiponectin concentrations in non-obese, obese, and type 2 diabetic subjects. Serum adiponectin concentrations were significantly lowered in diabetic subjects than non-diabetic subjects. Non-obese non-diabetic subjects showed higher adiponectin concentrations than those of obese subjects. *P< 0.05, **P< 0.01
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Fig. 2
Serum resistin concentrations in non-obese, obese, and type 2 diabetic subjects. Serum resistin concentrations were not different among non-obese, obese, and type 2 diabetic subjects.
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Fig. 3
Association between logarithmically transformed adiponectin concentrations and HOMA-IR (A) or BMI (B) in all subjects. There were negative correlations between HOMA-IR, BMI, and log adiponectin. Correlation coefficients and P values were calculated by Pearson correlation.
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Fig. 4
Association between logarithmically transformed resistin concentrations and HOMA-IR (A) or BMI (B) in all subjects. There were no correlations between HOMA-IR, BMI, and log resistin. Correlation coefficients and P values were calculated by Pearson correlation.
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Table 1
Baseline Characteristics of Study Subjects
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Results are expressed as the mean ± SD. *P<0.01 versus nonobese subjects; †P<0.01 versus obese subjects; ‡P<0.05 versus obese subjects. BMI: Body mass index, SC fat area: subcutaneous fat area, VSR: visceral fat vs. subcutaneous fat area ratio, HOMA-IR: homeostasis model assessment of insulin resistance.

Table 2
Multiple Regression Analysis of Anthropometric, Metabolic Factors as Predictors of Serum Adiponectin Levels
jkse-20-444-i002

BMI: Body mass index, SC fat area: subcutaneous fat area, HOMA-IR: homeostasis model assessment of insulin resistance.

Table 3
Multiple Regression Analysis of Anthropometric, Metabolic Factors as Predictors of Serum Resistin Levels
jkse-20-444-i003

BMI: Body mass index, SC fat area: subcutaneous fat area, HOMA-IR: homeostasis model assessment of insulin resistance

References

1. Tsao TS, Lodish HF, Freubis J. ACRP30, a new hormone controlling fat and glucose metabolism. Eur J Pharmacol. 2002. 440:213–221.
2. Weyer C, Funahashi T, Tanaka S, Hotta K, Matsuzawa Y, Pratley RE, Tataranni PA. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin endocrinol Metab. 2001. 86:1930–1935.
3. Cnop M, Havel PJ, Utzschneider KM, Carr DB, Sinha MK, Boyko EJ, Retzlaff BM, Knopp RH, Brunzell JD, Kahn SE. Relationship of adiponectin to body fat distribution, insulin sensitivity and plasma lipoproteins: evidence for independent roles of age and sex. Diabetologia. 2003. 46:459–469.
4. Hotta K, Funahashi T, Arita Y, Takahashi M, Matsuda M, Okamoto Y, Iwahashi H, Kuriyama H, Ouchi N, Maeda K, Nishida M, Kihara S, Sakai N, Nakajima T, Hasegawa K, Muraguchi M, Ohmoto Y, Nakamura T, Yamashita S, Hanafusa T, Matsuzawa Y. Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Biol. 2000. 20:1595–1599.
5. Mallamaci F, Zoccali C, Cuzzola F, Tripepi G, Cutrupi S, Parlongo S, Tanaka S, Ouchi N, Kihara S, Funahashi T, Matsuzawa Y. Adiponectin in essential hypertension. J Nephrol. 2002. 15:507–511.
6. Kumada M, Kihara S, Sumitsuji S, Kawamoto T, Matsumoto S, Ouchi N, Arita Y, Okamoto Y, Shimomura I, Hiraoka H, Nakamura T, Funahashi T, Matsuzawa Y. Osaka CAD Study Group. Coronary artery disease: Association of hypoadiponectinemia with coronary artery disease in men. Arterioscler Thromb Vasc Biol. 2003. 23:85–89.
7. Steppan CM, Bailey ST, Bhat S, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA. The hormone resistin link obesity to diabetes. Nature. 2001. 409:307–312.
8. Mcternan CL, Mcternan PG, Harte AL, Levick PL, Barnett AH, Kumar S. Resistin, central obesity, and type 2 diabetes. Lancet. 2002. 259:46–47.
9. Kim KH, Lee K, Moon YS, Sul HS. A cystein-rich adipose tissue-specific secretory factors inhibits adipocyte differentiation. J Biol Chem. 2001. 276:11252–11256.
10. Way JM, Gorgun CZ, Tong Q, Uysal KT, Brown KK, Harrington WW, Oliver JR, Wilson TM, Kliewer SA, Hotamisligil GS. Adipose tissue resistin expression is severly suppressed in obesity and stimulated by peroxisome proliferators-activated receptor α agonists. J Biol Chem. 2001. 276:25651–25653.
11. Savage DB, Sewter CP, Klenk ES, Segal DG, Vidal-Puig A, Considine RV, Orahilly S. Resistin/Fizz3 expression in relation to obesity and peroxisome proliferator-activated receptor- an action in human. Diabetes. 2001. 50:2199–2202.
12. Ahima RS, Flier JS. Adipose tissue as an endocrine organ. Trends Endocrinol Metab. 2000. 11:327–332.
13. Maeda N, Takahashi M, Funahashi T, Kihara S, Nishizawa H, Kishida K, Nagaretani H, Matsuda M, Komuro R, Ouchi N, Kuriyama H, Hotta K, Nakamura T, Shimomura I, Matsuzawa Y. PPARgamma ligands increase expression and plasma concentrations of adiponectin, an adipose-derived protein. Diabetes. 2001. 50:2094–2099.
14. Phillips SA, Ciaraldi TP, Kong AP, Bandukwala R, Aroda V, Carter L, Baxi S, Mudaliar SR, Henry RR. Modulation of circulating and adipose tissue adiponectin levels by antidiabetic therapy. Diabetes. 2003. 52:667–674.
15. Combs TP, Wagner JA, Berger J, Doebber T, Wang WJ, Zhang BB, Tanen M, Berg AH, O'Rahilly S, Savage DB, Chatterjee K, Weiss S, Larson PJ, Gottesdiener KM, Gertz BJ, Charron MJ, Scherer PE, Moller DE. Induction of adipocyte complement-related protein of 30kilodaltons by PPARgamma agonists: a potential mechanism of insulin sensitization. Endocrionology. 2002. 143:998–1007.
16. Berg AH, Combs TP, Du X, Brownlee M, Scherer PE. The adipocyte-secreted protein Acrp30 enhances hepatic insulin action. Nat Med. 2001. 7:947–953.
17. Fruebis J, Tsao TS, Javorschi S, Ebbets-Reed D, Erickson MR, Yen FT, Bihain BE, Lodish HF. Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice. Proc Natl Acad Sci U S A. 2001. 98:2005–2010.
18. Yamauchi T, Kamon J, Waki H, Terauchi Y, Kubota N, Hara K, Mori Y, Ide T, Murakami K, Tsuboyama-Kasaoka N, Ezaki O, Akanuma Y, Gavrillova O, Vinson C, Reitman ML, Kagechika H, Shudo K, Yoda M, Nakano Y, Tobe K, Nagai R, Kimura S, Tomita M, Froguel P, Kadowaki T. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med. 2001. 7:941–946.
19. Nishizawa T, Shimomura I, Kishida K, Maeda N, Kuriyama H, Nagaretani H, Matsuda M, Kondo H, Furuyama N, Kihara S, Nakamura T, Tochino Y, funahashi T, Matsuzawa Y. Androgens decrease plasma adiponectin, an insulin-sensitizing adipocyte derived protein. Diabetes. 2002. 51:2734–2741.
20. Lee JH, Chan JL, Yiannakouris N, Kontogianni M, Estrada E, Seip R, Orlova C, Mantzoros C. Circulating Resistin Levels Are Not Associated with Obesity or Insulin Resistance in Humans and Are Not Regulated by Fasting or Leptin Administration: Cross-Sectional and Interventional Studies in Normal, Insulin Resistant, and Diabetic Subjects. J Clin Endocrinol Metab. 2003. 88:4848–4856.
21. Youn BS, Yu KY, Park HJ, Lee NS, Min SS, Youn MY, Cho YM, Park YJ, Kim SY, Lee HK, Park KS. Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus. J Clin Endocrinol Metab. 2004. 89:150–156.
22. Jung HS, Youn BS, Cho YM, Yu KY, Park HJ, Shin CS, Kim SY, Lee HK, Park KS. The effects of rosiglitazone and metformin on the plasma concentrations of resistin in patients with type 2 diabetes mellitus. Metabolism. 2005. 54:314–320.
23. Fami JN, Cheema PS, Bahouth SW, Lloyd Hiler M. Resistin release by human adipose tissue explants in primary culture. Biochem Biophys Res Commun. 2003. 300:674–678.
24. Yang RZ, Huang Q, Xu A, McLenithan JC, Eisen JA, Shuldiner AR, Alkan S, Gong DW, eison JA. Comparative studies of resistin expression and phylogenomics in human and mouse. Biochem Biophys Res Commun. 2003. 310:927–935.
25. Patel L, Buckels AC, Kinghorn IJ, Murdock PR, Holbrook JD, Plumpton C, Macphee CH, Smith SA. Resistin is expressed in human macrophages and directly regulated by PPAR gamma activators. Biochem Biophys Res Commun. 2003. 300:472–276.
26. Kaser S, Kase A, Sandhofer A, Ebenbichler CF, Tilg H, Patsch JR. Resistin messenger-RNA expression is increased by proinflammatory cytokines in vitro. Biochem Biophys Res Commun. 2003. 309:286–290.
27. Reilly MP, Lehrke M, Wolfe ML, Rohatgi A, Lazar MA, Rader DJ. Resistin is an inflammatory markers of atherosclerosis in humans. Circulation. 2005. 111:932–939.
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