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Oh, Yun, Rhee, Lee, Baek, Kang, Park, Ihm, Choi, Yoo, and Park: The Effects of Osteoprotegerin Polymorphism on Bone Mineral Metabolism in Korean Women with Perimenopause
Original Article
Journal of Korean Society of Endocrinology

Journal of Korean Society of Endocrinology 2005; 20(3): 204-215.

Published online: 20 June 2005

DOI: https://doi.org/10.3803/jkes.2005.20.3.204

The Effects of Osteoprotegerin Polymorphism on Bone Mineral Metabolism in Korean Women with Perimenopause

Ki Won Oh, Eun Joo Yun1, Eun Jung Rhee2, Won Young Lee2, Ki Hyun Baek3, Moo Il Kang3, Cheol Young Park, Sung Hee Ihm, Moon Gi Choi, Hyung Joon Yoo, Sung Woo Park

Department of Internal Medicine, College of Medicine, Hallym University, Chunchon, Korea.

Department of Radiology1, College of Medicine, Hallym University, Chunchon, Korea.

Department of Internal Medicine2, Sungkyunkwan University School of Medicine, Seoul, Korea.

Department of Internal Medicine3, The Catholic University of Korea, College of Medicine, Seoul, Korea.

Received 18 February 2005       Accepted 16 March 2005

Copyright © 2005 Korean Endocrine Society

Abstract

Background

Osteoprotegerin (OPG) is a recently identified cytokine, which acts as a decoy receptor for the receptor activator of the NF-κB ligand (RANKL), and has also been shown to be an important inhibitor of osteoclastogenesis in animal models. However, the relationship between OPG gene polymorphism and female bone stati in human populations is unclear. In this study, the relationship between OPG gene polymorphisms and bone mineral metabolism in healthy Korean women was investigated.

Methods

We observed 251 healthy women (mean age, 51.3 ± 6.9 yr). The serum OPG concentrations were determined using ELISA, and the biochemical markers of bone turnover and FSH measured using standard methods. The bone mineral densities at the lumbar spine and femoral neck were measured by dual energy x-ray absorptiometry. The A163G, G209A, T245G and T950C polymorphisms of the OPG gene were analyzed by allelic discrimination using the 5 nuclease polymerase chain reaction assay.

Results

The lumbar spine BMD of premenopausal women was marginally decreased in the variant allele group compared to the wild type group (A163G, 0.98 ± 0.14 g/cm2 [GG + GA] vs. 1.05 ± 0.15 g/cm2 [AA], P = 0.070; T245G, 0.97 ± 0.13 g/cm2 [GG + GT] vs. 1.04 ± 0.15 g/cm2 [TT], P = 0.056). In the linkage of polymorphisms A163G and T245G, the lumbar spine BMD of premenopausal women was marginally decreased in the variant allele group compared to the wild type group ([AATT] vs. [AGTG + AGGG + GGTG + GGGG]: 1.04 ± 0.15 vs. 0.97 ± 0.13; P = 0.072). However, there were no differences in the serum OPG levels and bone turnover markers among the different genotypes.

Conclusion

The A163G and T245G polymorphisms of the OPG gene were observed to be marginally associated with the lumbar spine BMD in healthy premenopausal Korean women, but further studies will be needed to clarify this relationship

Keywords: Osteoprotegerin, Polymorphism, Bone mineral density

Figures and Tables

Fig. 1
Allelic discrimination using the 5 nuclease polymerase chain reaction assay
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Table 1
General Characteristics of the Subjects
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Data are given as mean values±SDs.

Table 2
Genotype Frequency of OPG Polymorphism
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Hardy-Weinberg equilibrium analyzed by chi-square test.

Table 3
Biochemical Marker of Bone Turnover according to A163G OPG Polymorphism
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Table 4
Biochemical Marker of Bone Turnover according to G209A OPG Polymorphism
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Table 5
Biochemical Marker of Bone Turnover according to T245G OPG Polymorphism
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Table 6
Biochemical Marker of Bone Turnover according to T950C OPG Polymorphism
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Table 7
Lumbar Spine and Femoral Neck Bone Mineral Density according to A163G OPG Polymorphism
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Table 8
Lumbar Spine and Femoral Neck Bone Mineral Density according to G209A OPG Polymorphism
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Table 9
Lumbar Spine and Femoral Neck Bone Mineral Density according to T245G OPG Polymorphism
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Table 10
Lumbar Spine and Femoral Neck Bone Mineral Density according to T950C OPG Polymorphism
jkse-20-204-i010

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