Journal List > J Korean Diabetes Assoc > v.31(4) > 1062453

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Kim, Chung, Ha, Kim, Nam, Lee, Kho, Shin, and Chung: The Effect of Rosiglitazone on Gluose Metabolism and Insulin Sensitivity in Non Obese Type 2 Diabetic Rat Models
Original Article
The Journal of Korean Diabetes Association

The Journal of Korean Diabetes Association 2007; 31(4): 319-325.

Published online: 31 July 2007

DOI: https://doi.org/10.4093/jkda.2007.31.4.319

The Effect of Rosiglitazone on Gluose Metabolism and Insulin Sensitivity in Non Obese Type 2 Diabetic Rat Models

Mi Jin Kim, Eui Jong Chung, Byung Wook Ha, Ji Hoon Kim, Su Min Nam1, Mi Young Lee1, Jang Hyun Kho1, Young Goo Shin1, Choon Hee Chung1

Department of Internal Medicine, Hong Ik Hospital, Korea.

1Department of Internal Medicine, Wonju College of Medicine, Yonsei University, Korea.

Received 14 February 2007       Accepted 9 May 2007

Copyright © 2007 Korean Diabetes Association

Abstract

Background

In Korea, most of type 2 diabetic patients are non obese. We made non obese type 2 diabetic rat models, which were characterized by insulin resistance and insulin secretion defect. Our study aimed to investigate the effect of rosiglitazone on glucose metabolism and insulin sensitivity in non obese type 2 diabetic rat models. Furthermore, we may estimate the effect of rosiglitazone treatment in non obese type 2 diabetic patients in Korea.

Methods

20 male newborn (12 hours old) Sprague-Dawley rats were made diabetes by streptozotocin (75 mg/kg, intraperitoneal injection). At 16 weeks old, diabetes were confirmed by intraperitoneal glucose tolerance test (IPGTT, 30% D/W, 2 kg/kg). After that, diabetic groups were divided into two groups. One group was fed on normal chow and rosiglitazone (3 mg/kg/day) and the other group was fed on normal chow for eight weeks. At the age of 24 weeks, we measured body weight (BW), plasma glucose, insulin, C-peptide levels. And we performed IPGTT and insulin tolerance test (ITT) in two groups. Thereafter, we determined the insulin content of pancreas and epididymal fat weight.

Results

Body weight was significantly higher in rosiglitazone group than control group. On IPGTT, plasma glucose, insulin and C-peptide levels were not significantly different between two groups. But, on insulin tolerance test, Kitt (%/min) values of rosiglitazone group were significantly higher than control group (2.7 vs. 1.8). The insulin content of pancreas and epididymal fat weight was not different between two groups.

Conclusion

These results suggested that rosiglitazone improved insulin sensitivity in non obese type 2 diabetes rat models independent of glucose level.

Keywords: Diabetes Mellitus, Insulin Sensitivity, Rosiglitazone

Figures and Tables

Fig. 1
Weight changes after eight weeks rosiglitazone treatment. *P < 0.05 between group A and group B.
jkda-31-319-g001
Fig. 2
Immunohistochemical staining (insulin antibody) of pancreatic islets (×100).
jkda-31-319-g002
Table 1
Intraperitoneal glucose tolerance test after eight weeks rosiglitazone treatment
jkda-31-319-i001

Data express mean ± SD.

Table 2
Insulin tolerance test after eight weeks rosiglitazone treatment
jkda-31-319-i002

Data express mean ± SD. *P < 0.05 between group A and group B.

Table 3
Light microscopic parameters of pancreatic islet cells after eight weeks rosiglitazone treatment
jkda-31-319-i003

Data express mean ± SD.

Table 4
Epididymal fat contents after eight weeks rosiglitazone treatment
jkda-31-319-i004

Data express mean ± SD.

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