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Kee, Shin, Chang, Chung, Shin, Kim, Chang, Kim, Kwak, and Kim: Identification of Tumor Suppressor Loci on the Short Arm of Chromosome 16 in Primary Small Cell Lung Cancers
Original Article

Tuberculosis and Respiratory Diseases 2003; 55(6): 597-611.

Published online: 31 December 2003

DOI: https://doi.org/10.4046/trd.2003.55.6.597

Identification of Tumor Suppressor Loci on the Short Arm of Chromosome 16 in Primary Small Cell Lung Cancers

Hyun Jung Kee2, Ju Hye Shin6, Joon Chang, M.D.1, 5, Kyung Young Chung, M.D.3, 5, Dong Hwan Shin, M.D.1, 5, Young Sam Kim, M.D.1, 5, Yoon Soo Chang, M.D.1, 5, Sung Kyu Kim, M.D.7, Seung Min Kwak, M.D., Se Kyu Kim, M.D.1, 2, 5, 6

1Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.

2Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea.

3Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.

4Department of Cardiovascular and Thoracic Surgery, Yonsei University College of Medicine, Seoul, Korea.

5The Institute of Chest Diseases, Yonsei University College of Medicine, Seoul, Korea.

6Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul, Korea.

7Department of Internal Medicine, Pulmonary Division, Inha University Hospital, 7-206, 3-ga, Shinheung-dong, Jung-gu, Incheon 400-103, Korea.

Address for correspondence: Se Kyu Kim, M.D. Department of Internal Medicine, Yonsei University College of Medicine CPO BOX 8044, Seoul, Korea. Phone: 82-2-361-5429, Fax: 82-2-393-6884, sekyukim@yumc.yonsei.ac.kr

Copyright©2003. The Korean Academy of Tuberculosis and Respiratory Diseases. All rights reserved.

Abstract

Background

Loss of the short arm of chromosome 16 is a frequent event in various cancers, which suggests the presence of tumor suppressor gene(s) there. To map precise tumor suppressor loci on the chromosome arm for further positional cloning efforts, we tested 23 primary small cell lung cancers.

Methods

The DNAs extracted from paraffin embedded tissue blocks with primary tumor and corresponding control tissue were investigated. Twenty polymorphic microsatellite markers located in the short arm of chromosome 16 were used in the microsatellite analysis.

Results

We found that six (26.1%) of 23 tumors exhibited LOH in at least one of tested microsatellite markers. Two (8.7%) of 6 tumors exhibiting LOH lost a larger area in chromosome 16p. LOH was observed in five common deleted regions at 16p. Among those areas, LOH between D16S668 and D16S749 was most frequent (21.1%). LOH was also observed at four other regions, between D16S3024 and D16S748, D16S405, D16S420, and D16S753. Six of 23 tumors exhibited shifted bands in at least one of the tested microsatellite markers. Shifted bands occurred in 3.3% (15 of 460) of the loci tested.

Conclusion

Our data demonstrated that at least five tumor suppressor loci might exist in the short arm of chromosome 16 and that they may play an important role in small cell lung cancer tumorigenesis.

Keywords: Small cell lung cancer, Chromosome 16, LOH, Tumor suppressor

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