Journal List > Tuberc Respir Dis > v.55(4) > 1062273

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Lee, Lee, Jung, Kim, Lee, Lee, Na, Jeon, Choi, Kim, Lee, and Park: Aberrant Promoter Methylation of Death-Associated Protein Kinase in Serum DNA from Lung Cancer Patients
Original Article

Tuberculosis and Respiratory Diseases 2003; 55(4): 378-387.

Published online: 31 October 2003

DOI: https://doi.org/10.4046/trd.2003.55.4.378

Aberrant Promoter Methylation of Death-Associated Protein Kinase in Serum DNA from Lung Cancer Patients

Jun Hee Lee, M.D., Jung Wook Lee, M.D., Kyung Sik Jung, M.D., Ki Uk Kim, M.D., Tae Kun Lee, M.D., Kyung Woo Lee, M.D., Min Ah Na, M.D., Doo Soo Jeon, M.D., Young Min Choi1, Yun Seong Kim, M.D., Min Ki Lee, M.D., Soon Kew Park, M.D.

Department of Internal Medicine, Pusan National University College of medicine, Busan, Korea.

1Medical Research Institute of Pusan National University Hospital, Busan, Korea.

Address for correspondence: Min Ki Lee, M.D. Department of Internal Medicine, Pusan National University College of Medicine 1-10, Ami-Dong, Suh-Gu, Busan 602-739, Korea. Phone: 051-240-7216, Fax: 051-254-3127, leemk@pusan.ac.kr

Copyright©2003. The Korean Academy of Tuberculosis and Respiratory Diseases. All rights reserved.

Abstract

Background

Promoter methylation of tumor suppressor genes is one of the key epigenetic changes in many human cancers. The aim of this study was to evaluate the promoter methylation status of the Death-associated protein(DAP) kinase gene, which played an important role in lung cancer, in the serum DNA of primary lung cancer patients.

Methods

This study investigated the aberrant methylation of DAP kinase in the serum of 65 primary lung cancer patients by methylation-specific PCR (MSP).

Results

Methylation in the serum was detected in 29 of 65(44.6%) for DAP kinase. There was no statistical association between methylation of DAP kinase and age, smoking history, histologic type, or stage. Methylation of DAP kinase was found more frequently in men (p=0.044).

Conclusions

This study suggests that the aberrant methylation of the DAP kinase promoter is readily detectable in the serum DNA of lung cancer patients using MSP analysis.

Keywords: Lung cancer, DAP kinase, Methylation, Serum DNA, MSP

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