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Kim, Kim, Yoon, Kim, Choi, Oh, Shim, Lim, Lee, Kim, Kim, Kim, and Koh: Role of Endogenous Histamine on the Pathogenesis in the Endotoxin-Induced Acute Lung Injury
Original Article

Tuberculosis and Respiratory Diseases 2003; 54(1): 91-103.

Published online: 31 January 2003

DOI: https://doi.org/10.4046/trd.2003.54.1.91

Role of Endogenous Histamine on the Pathogenesis in the Endotoxin-Induced Acute Lung Injury

Tae-Hyung Kim, M.D.1, Eun-Kyung Kim, M.D., Ho Joo Yoon, M.D.*, Mi Jung Kim, M.D.†, Jeoung Eun Choi, M.D., Yeon Mok Oh, M.D., Tae Sun Shim, M.D., Chae Man Lim, M.D., Sang Do Lee, M.D., Woo Sung Kim, M.D., Dong-Soon Kim, M.D., Won Dong Kim, M.D., Younsuck Koh, M.D.

1Department of Internal Medicine, Kwang-Myung Sung-ae General Hospital, Korea.

Divsision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Ulsan College of Medicine, Korea.

*Divsision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Han Yang University College of Medicine, Korea.

Divsision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Kwandong University College of Medicine, Korea.

†Asan Institute for Life Science, Seoul, Korea.

Address for correspondence: Younsuck Koh, M.D. Division of Pulmnoary and Critical Care Medicine 388-1 Pungnap Dong Songpa Ku, Seoul, 138-736, Korea. Phone: 02-3010-3134, Fax: 02-3010-6968, yskoh@amc.seoul.kr

Copyright©2003. The Korean Academy of Tuberculosis and Respiratory Diseases. All rights reserved.

Abstract

Background

Histamine is widely distributed in the lung. It increases capillary permeability and the P-selectin expression on vascular endothelial cell surfaces. We studied the role of endogenous histamine on the pathogenesis of endotoxin-induced acute lung injury (ALI) in rats.

Methods

We instilled either normal saline (control group) or lipopolysaccharide (3 mg/Kg, LPS group) to tracheas of Sprague-Dawley rats. H1-receptor blocker (mepyramine, 10 mg/Kg, H1RB group), H2-receptor blocker (ranitidine, 10 mg/Kg, H2RB group), and H3-receptor blocker (thioperamide, 2 mg/Kg, H3RB group) were administered through vein or peritoneum along with intratracheal LPS administration. Statistical significance was accepted at p<0.05.

Results

LPS increases the histamine level in BAL fluid significantly at 2 h after the treatment compared with control group. LPS significantly increases protein concentration, PMN cell count in bronchoalveolar lavage (BAL) fluid, and myeloperoxidase (MPO) activity in the lung tissue at 6 h compared to control group. PMN cell count in BAL fluid and MPO activity in lung tissue were significantly lower in H2RB-group compared to LPS-group. However, protein concentration in BAL fluid showed no significant differences between the LPS alone and LPS with histamine receptor blockade.

Conclusions

Endogenous histamine might be involved in the recruitment of PMNs in LPS-induced ALI via H2 receptor. However, its role in ALI would not be significant in this model.

Keywords: Histamine, Acute lung injury (ALI), Endotoxin, Histamine receptor blockades

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