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Cho, Quilantang, Lee, Kim, Kim, Cho, Jung, and Lee: Content Analysis of Rutin in the Leaves of Boehmeria nivea Harvested in Different Regions of South Korea by HPLC-UV
Original Article

Natural Product Sciences 2018;24(1):36-39.

Published online: 20 January 2018

DOI: https://doi.org/10.20307/nps.2018.24.1.36

Content Analysis of Rutin in the Leaves of Boehmeria nivea Harvested in Different Regions of South Korea by HPLC-UV

Sunghun Cho1, Norman G. Quilantang1, Ju Sung Lee1, Young-Mi Kim2, Ho Bang Kim3, Eun Ju Cho4, Yong-Su Jung2, Sanghyun Lee1, ∗

1Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea

2Yeong-Gwang Agricultural Technology Center, Yeonggwang 57031, Korea

3Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 14548, Korea

4Department of Food Science and Nutrition, Pusan National University, Busan 46241, Korea

∗Author for correspondence Sanghyun Lee, Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea Tel: +82-31-670-4688; E-mail: slee@cau.ac.kr

Received 5 September 2017       Revised 23 September 2017       Accepted 30 September 2017

Copyright © 2018 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Phytochemical analysis of Boehmeria nivea (Bn) leaves by medium pressure liquid chromatography led to the isolation of a flavonoid glycoside identified by spectroscopic analysis as rutin. The amount of rutin in the leaves of Bn harvested from nine regions in South Korea (Bn 1–9) which were collected on the months of June, July, August, and September was determined by HPLC-UV analysis. A gradient elution program that utilizes a Discovery® C18 (4.6 × 250 mm, 5 µm) column and mobile phase composed of 1% acetic acid-water: acetonitrile (90:10 to 60:40 for min) was followed. The injection volume and flow rate were 10 µl and 1 mL/ min, respectively. UV detection was set at 350 nm. Results show that Bn-8 harvested in September reported the highest content of rutin among the samples analyzed. This study provides a basis for the optimal harvest time of Bn which maximizes the yield of rutin.

Keywords: Boehmeria nivea, HPLC-UV, Rutin, Content analysis

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Fig. 1.
Structure of rutin.
nps-24-36f1.tif
Fig. 2.
Calibration curve of rutin.
nps-24-36f2.tif
Fig. 3.
HPLC chromatograms of rutin (A) and MeOH extract of B. nivea (B).
nps-24-36f3.tif
Table 1.
Collection areas for B. nivea
Sample Collection area
Bn-1 Seobang variety in Hansan, Seocheon
Bn-2 Taiwan variety in Duwon, Goheung
Bn-3 Ramie in Mangun, Muan
Bn-4 Local variety in Hakgyo, Hampyeong
Bn-5 Local variety in Seocheon-4
Bn-6 White Peel variety in Biin, Seocheon-2
Bn-7 Improved variety in Gwangju, Taiwan variety in Goheung
Bn-8 Natural cross of White Peel variety
Bn-9 Local variety in Baeksu, Yeonggwang
Table 2.
Rutin content of the MeOH extracts of B. nivea (Bn) collected from different regions and harvest time
Harvest Tim me Bn-1 Bn-2 Bn-3 Bn-4 Bn-5 Bn-6 Bn-7 Bn-8 Bn-9
June 2.79 ± 0.05 3.37 ± 0.00 3.36 ± 0.00 3.64 ± 0.01 2.78 ± 0.14 2.02 ± 0.00 3.05 ± 0.03 4.06 ± 0.00 4.29 ± 0.05
July 4.47 ± 0.07 4.24 ± 0.08 5.69 ± 0.01 8.89 ± 0.05 2.84 ± 0.04 1.93 ± 0.00 7.60 ± 0.18 6.51 ± 0.01 7.22 ± 0.08
August 1.88 ± 0.01 8.04 ± 0.00 5.96 ± 0.13 5.34 ± 0.13 3.56 ± 0.11 6.52 ± 0.01 2.33 ± 0.00 2.58 ± 0.00 3.15 ± 0.03
September 1.63 ± 0.04 8.28 ± 0.57 14.78 ± 0.12 14.77 ± 0.04 16.68 ± 0.10 19.49 ± 0.17 2.86 ± 0.14 27.03 ± 0.05 4.71 ± 0.00
Total 10.76 23.93 29.79 32.64 25.86 29.96 15.84 40.18 19.37

Data is represented as the mean ± SD (n = 3) in mg/g DW.

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