Journal List > Nat Prod Sci > v.23(4) > 1060682

Choi and Shin: Anti-inflammatory and Anti-bacterial Effects of Aloe vera MAP against Multidrug-resistant Bacteria


Multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa are highly dangerous nosocomial pathogens, cause the symptoms of skin infections, pressure sores, sepsis, blood stream and wound infections. Unfortunately, these pathogens are immune to the most common antibiotics, such as, carbapenem, aminoglycoside and fluoroquinolone. Therefore, it is imperative that new and effective antibiotics be developed. In the present study, the antimicrobial effects of Aloe vera MAP (modified Aloe polysaccharide) on Staphylococcus aureus and Bacillus subtilis, Escherichia coli and Enterobacter aerogenes, and clinical Pseudomonas aeruginosa and clinical Acinetobacter baumannii were comprehensibly investigated. Prior to the growth inhibition effect measurement and antibiotic disc diffusion assay on gram-positive and gram-negative bacteria and selected multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii, antimicrobial resistance screening was performed for the multidrug-resistant bacteria obtained from clinical isolates. The results for showed the Aloe vera MAP had a concentration-dependent effect on all of examined bacteria, particularly on Pseudomonas aeruginosa. Anti-inflammatory and anti-oxidant experiments were also performed dose dependently effects to confirm the beneficial physiological effects of Aloe vera MAP.


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Fig. 1.
Cytotoxicity of the Raw 264.7 cells were treated with Aloe vera MAP extracts. Experiments were performed by means of an MTT enzyme assay. Data are measured as percentage of the control. Error bars represent the mean ± standard deviation (∗P < 0.05).
Fig. 2.
Growth inhibition effect of Aloe vera MAP extract (AVE) in a variety of bacteria including multidrug-resistant resistant bacteria. Data was measured as a percentage of the control, i.e., the bacteria cultured in the pure medium without Aloe vera MAP extracts (∗P < 0.001, ∗P < 0.004, ∗P < 0.019).
Fig. 3.
Effect of Aloe vera MAP extract (AVE) on inhibitory of Nitric oxide (NO) production in LPS-stimulated Raw 264.7 cells. Data are measured as percentage of the control treated only with LPS. Error bars represent the mean ± standard deviation (∗P < 0.05).
Fig. 4.
Induction of MCP-1 (monocyte chemoattractant protein 1) in Raw 264.7 cells treated Aloe vera MAP extracts (AVE). Data are presented as mean ± standard deviations of three independent experiments (∗P < 0.05).
Fig. 5.
Inhibitory effect of the Ascorbic acid and Aloe vera MAP on DPPH free radicals. Data was measured as a percentage of the control. Error bars represent the mean ± standard deviation (∗P < 0.05).
Table 1.
The minimum inhibitory concentrations test (MICs) of five antibiotics and AVE (mg/ml) against bacterial strains S. aureus, multidrug-resistant bacteria P. aeruginosa, and A. baumanii
Strains MIC (µg/ml)
S. aureus 0.2 0.8 0.8 0.4 0.1 32
P. aeruginosa >128 >128 >128 64 64 128
A. baumanii >128 16 >128 4 >128 >128

GEN, gentamicin; MEM, meropenem; TOB, tobramycin; TIG, tigecycline; CIP, ciprofloxacin; AVE, Aloe vera MAP extract (mg/ml)

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