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YIN, YOON, YOON, AHN, and LEE: Quantitative Analysis and Validation of Hirsutenone and Muricarpone B from Fermented Alnus sibirica
Original Article

Natural Product Sciences 2017;23(2):146-150.

Published online: 20 January 2017

DOI: https://doi.org/10.20307/nps.2017.23.2.146

Quantitative Analysis and Validation of Hirsutenone and Muricarpone B from Fermented Alnus sibirica

Jun YIN, Ki Hoon YOON, Seong Hye YOON, Hye Shin AHN, Min Won LEE∗

Laboratory of Pharmacognosy and Natural Product Derived Medicine, College of Pharmacy, Chung-Ang University, Seoul 156–756, South Korea

∗Author for correspondence Prof. Dr. Min Won Lee, College of Pharmacy, Chung-Ang University, Seoul 156-756, South Korea Tel: +82-2-820-5602; E-mail: mwlee@cau.ac.kr

Received 17 March 2017       Revised 8 May 2017       Accepted 9 May 2017

Copyright © 2017 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Alnus sibirica (AS) geographically distributes in Korea, Japan, Northeast China and Russia. The bark of this plant had been used for antipyretic, expectorant, anti-phlogistic, antitussive, anti-asthmatic and as a health tea for alcoholism. Recently, we studied various biological activities of AS and the isolated diarylheptanoid. In present study, we conducted fermentation of AS (FAS) and isolated two diarylheptanoid (hirsutenone and muricarpone B). Moreover, we established the validation and contents determinations of the two compounds by HPLC on FAS.

Keywords: Alnus sibirica, Fermentation, HPLC, Validation, Content

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Fig. 1.
The structures of main components of FAS, oregonin, hirsutenone and muricarpone B.
nps-23-146f1.tif
Fig. 2.
HPLC pattern of FAS, AS and the specificity of hirsutenone and muricarpone B.
nps-23-146f2.tif
Fig. 3.
Linearity of hirsutenone.
nps-23-146f3.tif
Fig. 4.
Linearity of muricarpone B.
nps-23-146f4.tif
Table 1.
Analysis condition of HPLC
Column Thermo® Syncronis C18 HPLC column (5 µm, 250 × 4.6 mm)
Detector Waters 112 UV/VIS (280 nm)
  0 min 20 min 35 min 38 min 45 min 47 min 55 min
H2O 95 75 60 0 0 95 95
ACN 5 25 40 100 100 5 5
Table 2.
Content of hirsutenone and muricarpone B in extract of FAS and AS
Compound FAS (%) AS (%)
Hirsutenone 28.70 2.74
Muricarpone B 6.72 −
Table 3.
Linear ranges and LOQ of hirsutenone and muricarpone B
Compound Linear range (µg/mL) Response slope (a) Response Factor (b) Correlation Coefficient (R2) LOQ (µg/mL)
Hirsutenone 250∼2000 7587.2 −860963 0.9976 76.12
Muricarpone B 125∼1000 3998.8 26209 0.9999 4.49
Table 4.
Precision and accuracy of hirsutenone and muricarpone B
Compounds Conc. (µg/mL) Accuracy (%) Precision (c.v., %)
Inter-day Intra-day Inter-day Intra-day
Hirsutenone 2000 98.86 98.83 0.08 0.07
1000 105.73 105.71 0.33 0.35
500 97.49 97.32 1.07 0.90
250 91.20 91.56 1.99 1.42
Muricarpone B 1000 99.90 99.82 0.11 0.15
500 100.49 100.85 0.57 0.77
250 99.70 99.82 1.19 0.77
125 99.39 98.35 1.76 1.5
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