Synergetic Hepatoprotective Effects of Korean Red Ginseng and Pueraria Radix on the Liver Damaged-Induced by Carbon Tetrachloride (CCl4) in Mice

Seung Hwan Hwang; Zhiqiang Wang; II-Jun Kang; Soon Sung Lim

doi:10.20307/nps.2017.23.2.132

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Hwang, Wang, Kang, and Lim: Synergetic Hepatoprotective Effects of Korean Red Ginseng and Pueraria Radix on the Liver Damaged-Induced by Carbon Tetrachloride (CCl4) in Mice

Original Article

Natural Product Sciences 2017;23(2):132-138.

Published online: 20 January 2017

DOI: https://doi.org/10.20307/nps.2017.23.2.132

Synergetic Hepatoprotective Effects of Korean Red Ginseng and Pueraria Radix on the Liver Damaged-Induced by Carbon Tetrachloride (CCl₄) in Mice

Seung Hwan Hwang¹, Zhiqiang Wang¹, II-Jun Kang¹, Soon Sung Lim^1,^2,^3,^∗

¹Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea

²Institute of Korean Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea

³Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea

∗Author for correspondence Soon Sung Lim, Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea Tel: +82-33-248-2133; E-mail: limss@hallym.ac.kr

Received 6 February 2017 Revised 21 April 2017 Accepted 24 April 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was designed to investigate the synergetic hepatoprotective effects from a mixture of Korean Red Ginseng and Pueraria Radix on carbon tetrachloride (CCl₄)-induced hepatotoxicity in mice. Liver toxicity was induced by intraperitoneal administration of CCl₄ (0.6mg/kg) in 12groups of ICR mice. The negative control group was given CCl₄ without test samples and the normal group was given no treatment. Among treatment groups, the RGAP treatment (Korean Red ginseng acetic acid extract: Pueraria radix water extract, w/ w, 38.4:57.6) decreased CCl₄-elevated ALT (101.60 IU/L), AST (833.89 IU/L), and LDH (365.02 IU/L) levels in the serum, and increased the SOD (11.03 unit/mg protein) and CAT (0.37 unit/mg protein) levels and the LPO levels (59.09 µM/g tissue) more than that in the mice group with CCl₄-induced control group hepatotoxicity. These results suggest that administration of a mixture of Korean Red ginseng and Pueraria radix decreases CCl₄-induced liver damage and enhances antioxidant activity in mice and imply that administration of the mixture in a certain ratio is more effective than single administration of either Korean Red ginseng or Pueraria radix alone.

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Keywords: Carbon tetrachloride, Red Ginseng, Pueraria Radix, Hepatoprotective, Synergetic

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Fig. 1.

Body weight change after 10-day oral administration of Red ginseng and Pueraria radix extract ¹) Refer to Table 1. ²) Data are expressed as the mean ± SE (n = 7)

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Fig. 2.

Liver weight per 100 g of body weight, to examine the effect of CCl₄ and administration of extract of Red ginseng and Pueraria radix on the weight of the organ ¹) Refer to Table 1. ²) Data are expressed as the mean ± SE (n = 7) Statistical significance of differences was calculated between C group and experiment groups. ∗P <0.05, ∗∗P <0.01, ∗∗∗P <0.001.

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Fig. 3.

Effect of Red ginseng and Pueraria radix on the activity of serum alanine transaminase (a; ALT), aspartate transaminase (b; AST), and lactate dehydrogenase (c; LDH) in CCl₄-treated ICR mice ¹) Refer to Table 1 ²) Data are expressed as the mean ± SE (n = 7) Statistical significance of differences was calculated between C group and experiment groups. ∗P <0.05, ∗∗P <0.01, ∗∗∗P <0.001.

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Fig. 4.

Effect of Red ginseng and Pueraria radix on the activity of superoxide dismutase (a; SOD), catalase (b; CAT), and lipid peroxidase (c; LPO) in CCl₄-treated ICR mice ¹) Refer to Table 1 ²) Data are expressed as the mean ± SE (n = 7) Statistical significance of differences was calculated between C group and experiment groups. ∗P <0.05, ∗∗P <0.01, ∗∗∗P <0.001.

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Fig. 5.

Histopathological observation of liver tissues (H&E, × 200)

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Table 1.

Composition of oral administration experimental diets (%)

Ingredients	Groups
Ingredients	RGAP	RGW	RGA	PW	RGWPSM	RGWP-1	RGWP-2	RGWPM-1	RGWPA-2
RGAE¹⁾	38.4	−	96.0	−	−	−	−	−	−
RGWE²⁾	−	96.0	−	−	38.4	19.2	38.4	38.4	38.4
PWE³⁾	57.6	−	−	96.0	19.2	76.8	57.6	45.1	45.1
Vitamin complex	4.0	4.0	4.0	4.0	4.0	4.0	4.0	4.0	4.0
A. asiatica	−	−	−	−	−	−	−	−	12.5
Milk thistle	−	−	−	−	−	−	−	12.5	−
Shell extract	−	−	−	−	19.2	−	−	−	−
M. lusoria	−	−	−	−	19.2	−	−	−	−
Total (%)	100	100	100	100	100	100	100	100	100

¹⁾ RGAE: Red ginseng acid extract

²⁾ RGWE: Red ginseng water extract

³⁾ PWE: Pueraria radix water extract

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