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Hwang, Wang, Kang, and Lim: Synergetic Hepatoprotective Effects of Korean Red Ginseng and Pueraria Radix on the Liver Damaged-Induced by Carbon Tetrachloride (CCl4) in Mice
Original Article

Natural Product Sciences 2017;23(2):132-138.

Published online: 20 January 2017

DOI: https://doi.org/10.20307/nps.2017.23.2.132

Synergetic Hepatoprotective Effects of Korean Red Ginseng and Pueraria Radix on the Liver Damaged-Induced by Carbon Tetrachloride (CCl4) in Mice

Seung Hwan Hwang1, Zhiqiang Wang1, II-Jun Kang1, Soon Sung Lim1, 2, 3, ∗

1Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea

2Institute of Korean Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea

3Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea

∗Author for correspondence Soon Sung Lim, Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea Tel: +82-33-248-2133; E-mail: limss@hallym.ac.kr

Received 6 February 2017       Revised 21 April 2017       Accepted 24 April 2017

Copyright © 2017 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was designed to investigate the synergetic hepatoprotective effects from a mixture of Korean Red Ginseng and Pueraria Radix on carbon tetrachloride (CCl4)-induced hepatotoxicity in mice. Liver toxicity was induced by intraperitoneal administration of CCl4 (0.6mg/kg) in 12groups of ICR mice. The negative control group was given CCl4 without test samples and the normal group was given no treatment. Among treatment groups, the RGAP treatment (Korean Red ginseng acetic acid extract: Pueraria radix water extract, w/ w, 38.4:57.6) decreased CCl4-elevated ALT (101.60 IU/L), AST (833.89 IU/L), and LDH (365.02 IU/L) levels in the serum, and increased the SOD (11.03 unit/mg protein) and CAT (0.37 unit/mg protein) levels and the LPO levels (59.09 µM/g tissue) more than that in the mice group with CCl4-induced control group hepatotoxicity. These results suggest that administration of a mixture of Korean Red ginseng and Pueraria radix decreases CCl4-induced liver damage and enhances antioxidant activity in mice and imply that administration of the mixture in a certain ratio is more effective than single administration of either Korean Red ginseng or Pueraria radix alone.

Keywords: Carbon tetrachloride, Red Ginseng, Pueraria Radix, Hepatoprotective, Synergetic

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Fig. 1.
Body weight change after 10-day oral administration of Red ginseng and Pueraria radix extract 1) Refer to Table 1. 2) Data are expressed as the mean ± SE (n = 7)
nps-23-132f1.tif
Fig. 2.
Liver weight per 100 g of body weight, to examine the effect of CCl4 and administration of extract of Red ginseng and Pueraria radix on the weight of the organ 1) Refer to Table 1. 2) Data are expressed as the mean ± SE (n = 7) Statistical significance of differences was calculated between C group and experiment groups. ∗P <0.05, ∗∗P <0.01, ∗∗∗P <0.001.
nps-23-132f2.tif
Fig. 3.
Effect of Red ginseng and Pueraria radix on the activity of serum alanine transaminase (a; ALT), aspartate transaminase (b; AST), and lactate dehydrogenase (c; LDH) in CCl4-treated ICR mice 1) Refer to Table 1 2) Data are expressed as the mean ± SE (n = 7) Statistical significance of differences was calculated between C group and experiment groups. ∗P <0.05, ∗∗P <0.01, ∗∗∗P <0.001.
nps-23-132f3.tif
Fig. 4.
Effect of Red ginseng and Pueraria radix on the activity of superoxide dismutase (a; SOD), catalase (b; CAT), and lipid peroxidase (c; LPO) in CCl4-treated ICR mice 1) Refer to Table 1 2) Data are expressed as the mean ± SE (n = 7) Statistical significance of differences was calculated between C group and experiment groups. ∗P <0.05, ∗∗P <0.01, ∗∗∗P <0.001.
nps-23-132f4.tif
Fig. 5.
Histopathological observation of liver tissues (H&E, × 200)
nps-23-132f5.tif
Table 1.
Composition of oral administration experimental diets (%)
Ingredients Groups
RGAP RGW RGA PW RGWPSM RGWP-1 RGWP-2 RGWPM-1 RGWPA-2
RGAE1) 38.4 − 96.0 − − − − − −
RGWE2) − 96.0 − − 38.4 19.2 38.4 38.4 38.4
PWE3) 57.6 − − 96.0 19.2 76.8 57.6 45.1 45.1
Vitamin complex 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0
A. asiatica − − − − − − − − 12.5
Milk thistle − − − − − − − 12.5 −
Shell extract − − − − 19.2 − − − −
M. lusoria − − − − 19.2 − − − −
Total (%) 100 100 100 100 100 100 100 100 100

1) RGAE: Red ginseng acid extract

2) RGWE: Red ginseng water extract

3) PWE: Pueraria radix water extract

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